Updated on 2026/03/07

写真a

 
KITO KEIJI
 
Organization
Undergraduate School School of Agriculture Professor
Title
Professor
External link

Degree

  • 博士(理学) ( 東京大学 )

Research Interests

  • プロテオミクス

  • Budding yeast

  • 質量分析

  • 出芽酵母

  • Proteomics

  • Mass spectrometry

Research Areas

  • Life sciences / Cell biology

  • Life sciences / Systems genomics

Education

  • The University of Tokyo   Graduate School, Division of Science   Biological Chemistry

    1993.4 - 1995.3

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    Country/Region: Japan

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  • Tokyo Institute of Technology   Faculty of Science   Department of Biomechanism

    1989.4 - 1993.3

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    Country/Region: Japan

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Research History

  • Meiji University   School of Agriculture   Professor

    2021.10

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  • Associate Professor

    2016.10

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  • Lecturer

    2009.4 - 2016.9

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  • Assistant Professor

    2007.4 - 2009.3

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  • Research Assistant

    2004.1 - 2007.3

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  • Research Assistant

    2002.6 - 2003.12

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  • 第一製薬株式会社 創薬第二研究所

    1995.4 - 2002.5

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Professional Memberships

Papers

  • Functional shell matrix proteins tentatively identified by asymmetric snail shell morphology Reviewed International journal

    Akito Ishikawa, Keisuke Shimizu, Yukinobu Isowa, Takeshi Takeuchi, Ran Zhao, Keiji Kito, Manabu Fujie, Noriyuki Satoh, Kazuyoshi Endo

    Scientific Reports   10 ( 1 )   9768 - 9768   2020.12

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    Molluscan shell matrix proteins (SMPs) are essential in biomineralization. Here, we identify potentially important SMPs by exploiting the asymmetric shell growth in snail, Lymnaea stagnalis. Asymmetric shells require bilaterally asymmetric expression of SMP genes. We examined expression levels of 35,951 transcripts expressed in the left and right sides of mantle tissue of the pond snail, Lymnaea stagnalis. This transcriptome dataset was used to identify 207 SMPs by LC-MS/MS. 32 of the 207 SMP genes show asymmetric expression patterns, which were further verified for 4 of the 32 SMPs using quantitative PCR analysis. Among asymmetrically expressed SMPs in dextral snails, those that are more highly expressed on the left side than the right side are 3 times more abundant than those that are more highly expressed on the right than the left, suggesting potentially inhibitory roles of SMPs in shell formation. The 32 SMPs thus identified have distinctive features, such as conserved domains and low complexity regions, which may be essential in biomineralization.

    DOI: 10.1038/s41598-020-66021-w

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    Other Link: http://www.nature.com/articles/s41598-020-66021-w

  • Identification of TGFβ-induced proteins in non-endocrine mouse pituitary cell line TtT/GF by SILAC-assisted quantitative mass spectrometry. Reviewed International journal

    Takehiro Tsukada, Yukinobu Isowa, Keiji Kito, Saishu Yoshida, Seina Toneri, Kotaro Horiguchi, Ken Fujiwara, Takashi Yashiro, Takako Kato, Yukio Kato

    Cell and tissue research   376 ( 2 )   281 - 293   2019.5

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    TtT/GF is a mouse cell line derived from a thyrotropic pituitary tumor and has been used as a model of folliculostellate cells. Our previous microarray data indicate that TtT/GF possesses some properties of endothelial cells, pericytes and stem/progenitor cells, along with folliculostellate cells, suggesting its plasticity. We also found that transforming growth factor beta (TGFβ) alters cell motility, increases pericyte marker transcripts and attenuates endothelial cell and stem/progenitor cell markers in TtT/GF cells. The present study explores the wide-range effect of TGFβ on TtT/GF cells at the protein level and characterizes TGFβ-induced proteins and their partnerships using stable isotope labeling of amino acids in cell culture (SILAC)-assisted quantitative mass spectrometry. Comparison between quantified proteins from TGFβ-treated cells and those from SB431542 (a selective TGFβ receptor I inhibitor)-treated cells revealed 51 upregulated and 112 downregulated proteins (|log2| > 0.6). Gene ontology and STRING analyses revealed that these are related to the actin cytoskeleton, cell adhesion, extracellular matrix and DNA replication. Consistently, TGFβ-treated cells showed a distinct actin filament pattern and reduced proliferation compared to vehicle-treated cells; SB431542 blocked the effect of TGFβ. Upregulation of many pericyte markers (CSPG4, NES, ACTA, TAGLN, COL1A1, THBS1, TIMP3 and FLNA) supports our previous hypothesis that TGFβ reinforces pericyte properties. We also found downregulation of CTSB, EZR and LGALS3, which are induced in several pituitary adenomas. These data provide valuable information about pericyte differentiation as well as the pathological processes in pituitary adenomas.

    DOI: 10.1007/s00441-018-02989-2

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  • Insights into the evolution of shells and love darts of land snails revealed from their matrix proteins. Reviewed

    Shimizu K, Kimura K, Isowa Y, Oshima K, Ishikawa M, Kagi H, Kito K, Hattori M, Chiba S, Endo K

    Genome biology and evolution   11 ( 2 )   380 - 397   2018.11

  • Estimating the protein burden limit of yeast cells by measuring the expression limits of glycolytic proteins. Reviewed International journal

    Yuichi Eguchi, Koji Makanae, Tomohisa Hasunuma, Yuko Ishibashi, Keiji Kito, Hisao Moriya

    eLife   7   e34595   2018.8

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    The ultimate overexpression of a protein could cause growth defects, which are known as the protein burden. However, the expression limit at which the protein-burden effect is triggered is still unclear. To estimate this limit, we systematically measured the overexpression limits of glycolytic proteins in Saccharomyces cerevisiae. The limits of some glycolytic proteins were up to 15% of the total cellular protein. These limits were independent of the proteins' catalytic activities, a finding that was supported by an in silico analysis. Some proteins had low expression limits that were explained by their localization and metabolic perturbations. The codon usage should be highly optimized to trigger the protein-burden effect, even under strong transcriptional induction. The S-S-bond-connected aggregation mediated by the cysteine residues of a protein might affect its expression limit. Theoretically, only non-harmful proteins could be expressed up to the protein-burden limit. Therefore, we established a framework to distinguish proteins that are harmful and non-harmful upon overexpression.

    DOI: 10.7554/eLife.34595

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  • TGFβ signaling reinforces pericyte properties of the non-endocrine mouse pituitary cell line TtT/GF. Reviewed International journal

    Takehiro Tsukada, Saishu Yoshida, Keiji Kito, Ken Fujiwara, Hideji Yako, Kotaro Horiguchi, Yukinobu Isowa, Takashi Yashiro, Takako Kato, Yukio Kato

    Cell and tissue research   371 ( 2 )   339 - 350   2018.2

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    The non-endocrine TtT/GF mouse pituitary cell line was derived from radiothyroidectomy-induced pituitary adenoma. In addition to morphological characteristics, because the cells are S100β-positive, they have been accepted as a model of folliculostellate cells. However, our recent microarray analysis indicated that, in contrast to folliculostellate cells, TtT/GF cells might not be terminally differentiated, as they share some properties with stem/progenitor cells, vascular endothelial cells and pericytes. The present study investigates whether transforming growth factor beta (TGFβ) can elicit further differentiation of these cells. The results showed that canonical (Tgfbr1 and Tgfbr2) and non-canonical TGFβ receptors (Tgfbr3) as well as all TGFβ ligands (Tgfb1-3) were present in TtT/GF cells, based on reverse transcription PCR. SMAD2, an intercellular signaling molecule of the TGFβ pathway, was localized in the nucleus upon TGFβ signaling. Furthermore, TGFβ induced cell colony formation, which was completely blocked by a TGFβ receptor I inhibitor (SB431542). Real-time PCR analysis indicated that TGFβ downregulated stem cell markers (Sox2 and Cd34) and upregulated pericyte markers (Nestin and Ng2). Double immunohistochemistry using mouse pituitary tissue confirmed the presence of NESTIN/NG2 double-positive cells in perivascular areas where pericytes are localized. Our results suggest that TtT/GF cells are responsive to TGFβ signaling, which is associated with cell colony formation and pericyte differentiation. As pericytes have been shown to regulate angiogenesis, tumorigenesis and stem/progenitor cells in other tissues, TtT/GF cells could be a useful model to study the role of pituitary pericytes in physiological and pathological processes.

    DOI: 10.1007/s00441-017-2758-x

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  • Proteomics analysis for asymmetric inheritance of preexisting proteins between mother and daughter cells in budding yeast Reviewed

    Mitsuhiro Okada, Shunta Kusunoki, Yuko Ishibashi, Keiji Kito

    GENES TO CELLS   22 ( 6 )   591 - 601   2017.6

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    DOI: 10.1111/gtc.12497

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  • Autophosphorylation of Specific Threonine and Tyrosine Residues in Arabidopsis CERK1 is Essential for the Activation of Chitin-Induced Immune Signaling Reviewed

    Maruya Suzuki, Masatoshi Shibuya, Hikaru Shimada, Noriko Motoyama, Masato Nakashima, Shohei Takahashi, Kenkichi Suto, Issei Yoshida, Saki Matsui, Natsumi Tsujimoto, Mihoko Ohnishi, Yuko Ishibashi, Zui Fujimoto, Yoshitake Desaki, Hanae Kaku, Keiji Kito, Naoto Shibuya

    PLANT AND CELL PHYSIOLOGY   57 ( 11 )   2312 - 2322   2016.11

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    DOI: 10.1093/pcp/pcw150

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  • A strategy for absolute proteome quantification with mass spectrometry by hierarchical use of peptide-concatenated standards Reviewed

    Keiji Kito, Mitsuhiro Okada, Yuko Ishibashi, Satoshi Okada, Takashi Ito

    PROTEOMICS   16 ( 10 )   1457 - 1473   2016.5

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    DOI: 10.1002/pmic.201500414

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  • Yeast Interspecies Comparative Proteomics Reveals Divergence in Expression Profiles and Provides Insights into Proteome Resource Allocation and Evolutionary Roles of Gene Duplication Reviewed

    Keiji Kito, Haruka Ito, Takehiro Nohara, Mihoko Ohnishi, Yuko Ishibashi, Daisuke Takeda

    MOLECULAR & CELLULAR PROTEOMICS   15 ( 1 )   218 - 235   2016.1

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    DOI: 10.1074/mcp.M115.051854

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  • Proteome analysis of shell matrix proteins in the brachiopod Laqueus rubellus Reviewed

    Yukinobu Isowa, Isao Sarashina, Kenshiro Oshima, Keiji Kito, Masahira Hattori, Kazuyoshi Endo

    PROTEOME SCIENCE   13   21   2015.8

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    DOI: 10.1186/s12953-015-0077-2

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  • MafB protein stability is regulated by the JNK and ubiquitin-proteasome pathways

    Hiroshi Tanahashi, Keiji Kito, Takashi Ito, Katsuji Yoshioka

    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS   494 ( 1 )   94 - 100   2010.2

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    DOI: 10.1016/j.abb.2009.11.018

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  • Remodeling of the SCF complex-mediated ubiquitination system by compositional alteration of incorporated F-box proteins

    Mitsunori Kato, Keiji Kito, Kazuhisa Ota, Takashi Ito

    PROTEOMICS   10 ( 1 )   115 - 123   2010.1

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    DOI: 10.1002/pmic.200900497

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  • Absolute quantification of the budding yeast transcriptome by means of competitive PCR between genomic and complementary DNAs

    Fumihito Miura, Noriko Kawaguchi, Mikio Yoshida, Chihiro Uematsu, Keiji Kito, Yoshiyuki Sakaki, Takashi Ito

    BMC GENOMICS   9   574   2008.11

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    DOI: 10.1186/1471-2164-9-574

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  • A proteomic screen reveals the mitochondrial outer membrane protein Mdm34p as an essential target of the F-box protein Mdm30p

    Kazuhisa Ota, Keiji Kito, Satoshi Okada, Takashi Ito

    GENES TO CELLS   13 ( 10 )   1075 - 1085   2008.10

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    DOI: 10.1111/j.1365-2443.2008.01228.x

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  • A parallel affinity purification method for selective isolation of polyubiquitinated proteins

    Kazuhisa Ota, Keiji Kito, Shun-ichiro Iemura, Tohru Natsume, Takashi Ito

    PROTEOMICS   8 ( 15 )   3004 - 3007   2008.8

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    DOI: 10.1002/pmic.200800271

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  • Discrimination between stable and dynamic components of protein complexes by means of quantitative proteomics

    Keiji Kito, Noriko Kawaguchi, Satoshi Okada, Takashi Ito

    PROTEOMICS   8 ( 12 )   2366 - 2370   2008.6

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    DOI: 10.1002/pmic.200800182

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  • Mass spectrometry-based approaches toward absolute quantitative proteomics

    Keiji Kito, Takashi Ito

    CURRENT GENOMICS   9 ( 4 )   263 - 274   2008.6

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    DOI: 10.2174/138920208784533647

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  • A synthetic protein approach toward accurate mass spectrometric quantification of component stoichiometry of multiprotein complexes

    Keiji Kito, Kazuhisa Ota, Tomoko Fujita, Takashi Ito

    JOURNAL OF PROTEOME RESEARCH   6 ( 2 )   792 - 800   2007.2

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    DOI: 10.1021/pr060447s

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  • Methods for protein-protein interaction analysis Reviewed

    Kito K, Ito T

    Introduction to Systems Biology   160 - 182   2007

  • The yeast eIF4E-associated protein Eap1p attenuates GCN4 translation upon TOR-inactivation

    R Matsuo, H Kubota, T Obata, K Kito, K Ota, T Kitazono, S Ibayashi, T Sasaki, M Iida, T Ito

    FEBS LETTERS   579 ( 11 )   2433 - 2438   2005.4

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    DOI: 10.1016/j.febslet.2004.03.043

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  • Mass spectrometry-based proteomics for quantitative description of cellular events

    K Kito, T Ito

    CURRENT GENOMICS   5 ( 8 )   629 - 635   2004.12

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  • Nonrequirement of continuous stimulation with MCP-1 for cell migration and determination of directional migration by initial stimulation with chemokine

    K Kito, K Nishida

    EXPERIMENTAL CELL RESEARCH   281 ( 1 )   157 - 166   2002.11

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    DOI: 10.1006/excr.2002.5663

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  • MCP-1 receptor binding affinity is up-regulated by pre-stimulation with MCP-1 in an actin polymerization-dependent manner

    K Kito, K Morishita, K Nishida

    JOURNAL OF LEUKOCYTE BIOLOGY   69 ( 4 )   666 - 674   2001.4

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  • Fluorescent differential display analysis of gene expression in differentiating neuroblastoma cells

    K Kito, T Ito, Y Sakaki

    GENE   184 ( 1 )   73 - 81   1997.1

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  • DIFFERENTIAL DISPLAY ANALYSIS OF GENE-EXPRESSION IN DEVELOPING EMBRYOS OF XENOPUS-LAEVIS

    N ADATI, T ITO, C KOGA, K KITO, Y SAKAKI, K SHIOKAWA

    BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION   1262 ( 1 )   43 - 51   1995.5

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  • FLUORESCENT DIFFERENTIAL DISPLAY - ARBITRARILY PRIMED RT-PCR FINGERPRINTING ON AN AUTOMATED DNA SEQUENCER

    T ITO, K KITO, N ADATI, Y MITSUI, H HAGIWARA, Y SAKAKI

    FEBS LETTERS   351 ( 2 )   231 - 236   1994.9

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  • MONOCLONAL-ANTIBODY (VOM2) SPECIFIC FOR THE LUMINAL SURFACE OF THE RAT VOMERONASAL SENSORY EPITHELIUM

    T OSADA, K KITO, K OOKATA, PPC GRAZIADEI, A IKAI, M ICHIKAWA

    NEUROSCIENCE LETTERS   170 ( 1 )   47 - 50   1994.3

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MISC

Presentations

  • 熱耐性に関わる新規タンパク質を特定するための酵母種間での比較プロテオミクス

    古澤和俊, 石橋裕子, 鳥居幸也, 紀藤圭治

    第39回日本分子生物学会年会  2016.12  日本分子生物学会

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  • 出芽酵母におけるプロテオーム資源分配の最適化と細胞増殖能との関係

    寺川瑛, 畔上楓, 石橋裕子, 紀藤圭治

    第39回日本分子生物学会年会  2016.12  日本分子生物学会

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    Venue:横浜市  

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  • 出芽酵母における老化タンパク質の分裂寿命への影響

    杉山知史, 岡田 充弘, 楠竣太, 陳思キ, 紀藤圭治

    第39回日本分子生物学会年会  2016.12  日本分子生物学会

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    Venue:横浜市  

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  • 出芽酵母の細胞分裂におけるタンパク質不均等分配のプロテオミクス解析

    岡田充弘, 楠竣太, 杉山知史, 石橋裕子, 紀藤圭治

    第39回日本分子生物学会年会  2016.12  日本分子生物学会

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  • 質量分析と安定同位体を用いたタンパク質の量的および質的解析方法

    紀藤圭治, 岡田充弘

    日本遺伝学会第88回大会  2016.9 

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  • 出芽酵母における代謝および翻訳へのプロテオーム資源分配と細胞増殖との関係

    寺川瑛, 石橋裕子, 紀藤圭治

    酵母遺伝学フォーラム第49回研究報告会  2016.9 

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  • Old-age proteins asymmetrically inherited in mother cells of budding yeast. International conference

    Keiji Kito, Mitsuhiro Okada, Shunta Kusunoki, Satoshi Sugiyama, Yuko Ishibashi

    15th Human Proteome Organization World Congress  2016.9  HUPO

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    Venue:Taipei, Taiwan  

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  • 質量分析を用いた細胞分裂時におけるタンパク質不均等分配の網羅的解析

    岡田充弘, 楠竣太, 杉山知史, 石橋裕子, 紀藤圭治

    酵母遺伝学フォーラム第49回研究報告会  2016.9 

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  • 酵母種間での比較プロテオーム解析による熱耐性に関わるタンパク質の探索

    古澤和俊, 石橋裕子, 鳥居幸也, 紀藤圭治

    酵母遺伝学フォーラム第49回研究報告会  2016.9 

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  • 酵母種間の比較プロテオミクス

    日本プロテオーム学会2016年大会  2016.7 

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  • 出芽酵母におけるタンパク質不均等分配のプロテオーム解析

    岡田充弘, 楠俊太, 杉山知史, 石橋裕子, 紀藤圭治

    日本プロテオーム学会2016年大会  2016.7 

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  • 出芽酵母におけるプロテオーム資源分配最適化の細胞増殖能への影響

    寺川瑛, 石橋裕子, 紀藤圭治

    日本プロテオーム学会2016年大会  2016.7 

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  • 酵母種間でのプロテオーム比較解析による熱耐性に関わるタンパク質の探索

    古澤和俊, 石橋裕子, 鳥居幸也, 紀藤圭治

    日本プロテオーム学会2016年大会  2016.7 

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  • 出芽酵母の細胞分裂におけるタンパク質不均等分配のプロテオミクス解析

    岡田充弘, 楠俊太, 杉山知史, 石橋裕子, 紀藤圭治

    第38回日本分子生物学会年会  2015.12 

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  • S. cerevisiaeとC. glabrataにおける熱ストレス耐性に関わるプロテオーム発現プロファイルの比較解析

    古澤和俊, 石橋裕子, 寺川瑛, 鳥居幸也, 紀藤圭治

    第38回日本分子生物学会年会  2015.12 

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  • 出芽酵母における遺伝子重複によるタンパク質発現量への影響

    矢島宙岳, 完戸麻里香, 石橋裕子, 伊藤遼, 野原健弘, 紀藤圭治

    第38回日本分子生物学会年会  2015.12 

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  • A strategy for large-scale analysis of asymmetric inheritance of old-age proteins at cell division International conference

    Keiji Kito, Mitsuhiro Okada, Shunta Kusunoki, Yuko Ishibashi

    Human Proteome Organization 14th World Congress  2015.10 

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  • 様々な生育条件下でのS. cerevisiaeとC. glabrataの比較プロテオーム解析

    古澤和俊, 石橋裕子, 武田大祐, 紀藤圭治

    酵母遺伝学フォーラム第48回研究報告会  2015.9 

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  • 出芽酵母を用いたタンパク質不均等分配の網羅的解析

    岡田充弘, 楠俊太, 杉山知史, 石橋裕子, 紀藤圭治

    酵母遺伝学フォーラム第48回研究報告会  2015.9 

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  • PCS-MS法による酵母種間における重複遺伝子発現量の比較解析

    矢島宙岳, 尾松祐太, 完戸麻里香, 石橋裕子, 伊藤遼, 野原健弘, 紀藤圭治

    日本プロテオーム学会2015年大会  2015.7 

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  • 異なる炭素源や熱ストレス存在下でのS. cerevisiaeとC. glabrataのプロテオームの比較解析

    古澤和俊, 伊藤遼, 野原健弘, 矢島宙岳, 石橋裕子, 大西美帆子, 武田大祐, 紀藤圭治

    日本プロテオーム学会2015年大会  2015.7 

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  • 酵母種間におけるタンパク質発現プロファイルの比較解析

    古澤和俊, 武田大祐, 伊藤遼, 大西美帆子, 野原健弘, 佐賀柾孝, 矢島宙岳, 紀藤圭治

    第37回日本分子生物学会年会  2014.11 

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  • 出芽酵母を用いたタンパク質不均等分配の質量分析による網羅的解析

    岡田 充弘, 佐藤 慶, 楠 竣太, 武田 大佑, 紀藤圭治

    第37回日本分子生物学会年会  2014.11 

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  • Conserved and diverse aspect of proteome profile across multiple yeast species International conference

    Keiji Kito, Haruka Ito, Takehiro Nohara, Mihoko Ohnishi, Daisuke

    13th Human Proteome Organization World Congress  2014.10 

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  • 酵母種間で代謝酵素群と重複遺伝子の発現プロファイルはどのくらい似ているか

    古澤和俊, 野原健弘, 伊藤遼, 大西美帆子, 武田大祐, 紀藤圭治

    酵母遺伝学フォーラム第47回研究報告会  2014.9 

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  • 質量分析を用いた酵母種間の比較プロテオミクス

    古澤和俊, 武田大祐, 伊藤遼, 大西美帆子, 野原健弘, 紀藤圭治

    日本プロテオーム学会2014年大会  2014.7 

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  • 定量解析からみた酵母種間におけるプロテオームの保存性と多様性

    日本プロテオーム学会2014年大会  2014.7 

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    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

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  • 出芽酵母を用いたタンパク質不均等分配の質量分析による網羅的解析

    岡田充弘, 佐藤慶, 武田大佑, 紀藤圭治

    日本プロテオーム学会2014年大会  2014.7 

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  • 質量分析による酵母種間でのプロテオーム解析

    野原健弘, 大西美帆子, 伊藤遼, 武田大佑, 古澤和俊, 井ノ口頼哉, 紀藤圭治

    第36回日本分子生物学会年会  2013.12 

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  • Comparative proteomics for yeast interspecies differences in metabolic pathway and regulation of protein expression International conference

    Mihoko Ohnishi, Haruka Ito, Takehiro Nohara, Keiji Kito

    Human Proteome Organization 12th Annual World Congress  2013.9 

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  • Comparative proteomics of yeast interspecies differences in metabolic pathway

    Mihoko Ohnishi, Haruka Ito, Yusuke Hasegawa, Kazushi Suzuki, Takehiro Nohara, Keiji Kito

    The 35th Annual Meeting of the Molecular Biology Society of Japan  2012.12  The Molecular Biology Society of Japan

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  • Comparative proteomics of yeast interspecies differences in protein expression

    Mihoko Ohnishi, Haruka Ito, Yusuke Hasegawa, Shoichiro Masaoka, Keiji Kito

    2012 Annual Meeting of Japan Human Proteome Organization  2012.7  Japan Human Proteome Organization

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  • Hierarchical Use of Peptide-concatenated Standard (PCS) for Absolute Protein Quantification with Mass Spectrometry International conference

    Keiji Kito, Takashi Ito

    Human Proteome World Congress Sydney 2010  2010.9 

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  • ペプチド連結型標準物質と質量分析(PCS-MS法)を用いたタンパク質の絶対量計測技術の開発

    紀藤圭治, 伊藤隆司

    日本プロテオーム機構・第6回大会  2009.7 

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Research Projects

  • 大規模オミックスの活用による生殖内分泌組織の新たな機能制御法の確立

    2014.4

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    Grant type:Competitive

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  • 定量的比較プロテオミクスによる生体内代謝経路のデザインと代謝キャパシティーの解析

    2013.4 - 2016.3

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    Grant type:Competitive

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  • 酵母におけるプロセス負荷の原理の解明

    2013.4 - 2015.3

    挑戦的萌芽研究 

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    Grant type:Competitive

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  • 下垂体の発生・分化・再生と生殖機能調節機構の解析

    2010.4 - 2013.3

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    Grant type:Competitive

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  • 絶対量計測に基づく比較プロテオミクスによる生体内代謝経路の制御機構の解明

    2010.4 - 2013.3

    基盤研究 (C) 

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    Grant type:Competitive

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  • PCS-MS法を用いた絶対定量解析による細胞周期分子システムの解明

    2009.4 - 2011.3

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    Grant type:Competitive

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  • 生体分子パスウェイ・ネットワークの定量計測技術と撹乱法の開発

    2009.4 - 2010.3

    特定領域研究 

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    Grant type:Competitive

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  • 全mRNAおよびタンパク質の絶対量計測のための基盤技術開発

    2007.4 - 2008.3

    特定領域研究 

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    Grant type:Competitive

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  • mRNAカウンティングの基盤技術開発

    2006.4 - 2007.3

    特定領域研究 

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    Grant type:Competitive

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