Updated on 2026/03/07

写真a

 
ASANUMA NARITO
 
Organization
Undergraduate School School of Agriculture Professor
Title
Professor
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Degree

  • 農学博士 ( 2001.3   明治大学大学院 )

Research Areas

  • Life sciences / Animal production science  / 畜産学・獣医学

  • Life sciences / Applied microbiology

Education

  • 明治大学大学院   農学研究科

    - 2001.3

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    Country/Region： Japan

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  • Meiji University   Faculty of Agriculture

    - 1996.3

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    Country/Region： Japan

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Research History

  • 日本学術振興会   日本学術振興会特別研究員   特別研究員-PD

    2001.4 - 2003.3

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  • Special researcher of the Japan Society for the Promotion of Science

    2001.4 - 2003.3

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  • Special researcher of the Japan Society for the Promotion of Science

    1998.4 - 2001.3

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  • 日本学術振興会   日本学術振興会特別研究員   特別研究員-DC1

    1998.4 - 2001.3

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Professional Memberships

Committee Memberships

  • 日本畜産学会   理事  

    2021.4   

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    Committee type：Academic society

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  • 関東畜産学会   会長  

    2021.4   

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    Committee type：Academic society

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  • 日本ルーメン研究会   会計  

    2005   

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    Committee type：Academic society

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Papers

  • イヌにおける共役リノール酸生成菌の生息数と加齢との関係 Reviewed

    鈴木慎人・信川将平・浅沼成人

    71   53 - 61   2021.9

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    Authorship：Last author,　Corresponding author   Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：関東畜産学会報  

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  • イヌ由来の共役リノール酸生成菌の単離とその特性解析 Reviewed

    信川将平・浅沼成人

    関東畜産学会報   71   41 - 52   2021.9

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    Authorship：Last author,　Corresponding author   Language：Japanese   Publishing type：Research paper (scientific journal)  

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  • 共役リノール酸を生成する<I>Butyrivibrio fibrisolvens</I>の腸内環境での生育に関係した特性 Reviewed

    鈴木慎人・信川将平・磯崎涼平・湯山星加・浅沼成人

    関東畜産学会報   70   9 - 18   2020.2

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    鈴木慎人・信川将平・磯崎涼平・湯山星加・浅沼成人

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  • 新規グルコシルセラミド水解菌Glucerabacter canisensisがイヌの大腸内で増殖するためのアミノ酸源およびエネルギー源としてのイヌ糞の評価 Reviewed

    川田実生・湯山星加・河原夏希・阿久津翔子・浅沼成人

    関東畜産学会報   69   82 - 90   2019.8

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  • Augmentation of dietary glucosylceramide hydrolysis by the novel bacterium <I>Glucerabacter canisensis</I> NATH-2371<SUP>T</SUP> Reviewed

    Misho Kawata・Masato Suzuki・Shoko Akutsu・Natsuki Kawahara・Ami Tsukamoto・Shohei Nobukawa・Ryohei Isozaki・Seika Yuyama・Narito Asanuma

    Bioscience, Biotechnology, and Biochemistry   82   2191 - 2197   2018.12

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  • Glucerabacter canisensis gen. nov., sp. nov., isolated from dog feces and its effect on the hydrolysis of plant glucosylceramide in the intestine of dogs Reviewed

    Misho Kawata, Ami Tsukamoto, Ryohei Isozaki, Shohei Nobukawa, Natsuki Kawahara, Shoko Akutsu, Masato Suzuki, Narito Asanuma

    Archives of Microbiology   200 ( 3 )   505 - 515   2018.4

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Verlag  

    DOI： 10.1007/s00203-017-1463-1

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  • Effects of dietary glucosylceramide on the improvement of skin barrier function and the alleviation of experimental bowel inflammation Reviewed

    65 ( 4 )   83 - 93   2016.3

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  • Effects of nitrate addition to a diet on fermentation and microbial populations in the rumen of goats, with special reference to Selenomonas ruminantium having the ability to reduce nitrate and nitrite Reviewed

    Narito Asanuma, Shota Yokoyama, Tsuneo Hino

    ANIMAL SCIENCE JOURNAL   86   378 - 384   2015.4

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/asj.12307

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  • Effects of oral administration of Butyrivibrio fibrisolvens MDT-1 on the development and healing of atopic dermatitis in NC/Nga mice Reviewed

    Tsuneo Hino, Kenta Suzuki, Sou Ohkawara, Takehiro Miwa, Narito Asanuma

    European Journal of Dermatology   22   211 - 217   2012

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    DOI： 10.1684/ejd.2011.1618

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  • Responses in digestion, rumen fermentation and microbial populations to inhibition of methane formation by a halogenated methane analogue. Reviewed

    Mitsumori M, Shinkai T, Takenaka A, Enishi O, Higuchi K, Kobayashi Y, Nonaka I, Asanuma N, Denman SE, McSweeney CS.

    British Journal of Nutrition   108 ( 3 )   482 - 491   2012

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    DOI： 10.1017/S0007114511005794

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  • Involvement of two-component signal transduction system, ComDE, in the regulation of growth and genetic transformation, in the ruminal bacterium Streptococcus bovis

    Narito Asanuma, Takahiro Yoshii, Kazuhiro Kanada, Kimio Yoshizawa, Yusuke Arai, Tatsuya Ichikawa, Akiko Kawamura, Tsuneo Hino

    ANAEROBE   16 ( 4 )   405 - 411   2010.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.anaerobe.2010.05.005

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  • Isolation of a novel bacterium, Blautia glucerasei sp nov., hydrolyzing plant glucosylceramide to ceramide

    Hideki Furuya, Yukari Ide, Makiko Hamamoto, Narito Asanuma, Tsuneo Hino

    ARCHIVES OF MICROBIOLOGY   192 ( 5 )   365 - 372   2010.5

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    DOI： 10.1007/s00203-010-0566-8

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  • Molecular characterization and significance of phosphoenolpyruvate carboxykinase in a ruminal bacterium, Streptococcus bovis

    Narito Asanuma, Kazuhiro Kanada, Yusuke Arai, Kimio Yoshizawa, Tatsuya Ichikawa, Tsuneo Nino

    JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY   56 ( 2 )   121 - 127   2010.4

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  • Presence of the two-component regulatory system ComDE, and its transcription in the ruminal bacterium Streptococcus bovis.

    Yoshii, T., N. Asanuma, K. Yoshizawa, Y. Arai, K. Kanada and T. Hino.

    Bulletin of School of Agriculture Meiji University   59   71-83   2009.12

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  • Molecular and Biochemical Characterization of Phosphoenolpyruvate Carboxykinase in the Ruminal Bacterium Ruminococcus albus

    Narito Asanuma, Kimio Yoshizawa, Kazuhiro Kanada, Tsuneo Hino

    CURRENT MICROBIOLOGY   58 ( 5 )   416 - 420   2009.5

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    DOI： 10.1007/s00284-008-9325-3

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  • Properties and Role of Glyceraldehyde-3-Phosphate Dehydrogenase in the Control of Fermentation Pattern and Growth in a Ruminal Bacterium, Streptococcus bovis

    Narito Asanuma, Kimio Yoshizawa, Tsuneo Hino

    CURRENT MICROBIOLOGY   58 ( 4 )   283 - 287   2009.4

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    DOI： 10.1007/s00284-008-9326-2

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  • Effect of oral administration of butyrivibrio fibrisolvens MDT-1 on experimental enterocolitis in mice (Clinical and Vaccine Immunology (2006) 13:11 (1231-1236))

    Sou Ohkawara, Hideki Furuya, Kousuke Nagashima, Narito Asanuma, Tsuneo Hino

    Clinical and Vaccine Immunology   16 ( 2 )   291   2009.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1128/CVI.00400-08

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  • Molecular properties and transcriptional control of the phosphofructokinase and pyruvate kinase genes in a ruminal bacterium, Streptococcus bovis

    Narito Asanuma, Kazuhiro Kanada, Tsuneo Hino

    ANAEROBE   14 ( 4 )   237 - 241   2008.10

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    DOI： 10.1016/j.anaerobe.2008.05.004

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  • Evaluation of dog feces as the amino acid source for the growth of Butyrivibrio fibrisolvens in the large intestine of dogs.

    Asanuma, N., K. Yoshizawa and T. Hino.

    Bulletin of School of Agriculture Meiji University   57   123-133   2008.1

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  • Dietary sphingomyelin alleviates experimental inflammatory bowel disease in mice

    Hideki Furuya, Sou Ohkawara, Kousuke Nagashima, Narito Asanuma, Tsuneo Hino

    INTERNATIONAL JOURNAL FOR VITAMIN AND NUTRITION RESEARCH   78 ( 1 )   41 - 49   2008.1

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    DOI： 10.1024/0300-9831.78.1.41

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  • スフィンゴ脂質の経口投与による疾病の予防・抑制と腸内細菌によるその効果の増強に関する最近の研究動向と展望

    古谷英樹・大河原壮・長島康祐・浅沼成人・日野常男

    明治大学農学部研究報告   57   93-97   2008.1

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  • Properties and role of pyruvate kinase in a ruminal bacterium, Streptococcus bovis.

    Asanuma, N., K. Kanada and T. Hino.

    Bulletin of School of Agriculture, Meiji University   57   59-66   2007.12

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  • Polysaccharides supporting the growth of Butyrivibrio fibrisolvens preferentially in cultures of mixed intestinal bacteria from dogs.

    Asanuma, N., T. Yoshii and T. Hino.

    Journal of Pet Animal Nutrition   10   88-96   2007.10

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  • Purification and gene sequencing of conjugated linoleic acid reductase from a gastrointestinal bacterium, Butyrivibrio fibrisolvens

    S. Fukuda, Y. Suzuki, T. Komori, K. Kawamura, N. Asanuma, T. Hino

    JOURNAL OF APPLIED MICROBIOLOGY   103 ( 2 )   365 - 371   2007.8

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    DOI： 10.1111/j.1365-2672.2006.03247.x

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  • Effect of oral administration of Butyrivibrio fibrisolvens MDT-1, a gastrointestinal bacterium, on 3-methylcholanthrene-induced tumor in mice

    Sou Ohkawara, Hideki Furuya, Kousuke Nagashima, Narito Asanuma, Tsuneo Hino

    NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL   59 ( 1 )   92 - 98   2007

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  • Effect of oral administration of Butyrivibrio fibrisolvens MDT-1 on experimental enterocolitis in mice

    Sou Ohkawara, Hideki Furuya, Kousuke Nagashima, Narito Asanuma, Tsuneo Hino

    CLINICAL AND VACCINE IMMUNOLOGY   13 ( 11 )   1231 - 1236   2006.11

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    DOI： 10.1128/CVI.00267-06

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  • Isolation of a novel strain of Butyrivibrio fibrisolvens that isomerizes linoleic acid to conjugated linoleic acid without hydrogenation, and its utilization as a probiotic for animals

    S Fukuda, Y Suzuki, M Murai, N Asanuma, T Hino

    JOURNAL OF APPLIED MICROBIOLOGY   100 ( 4 )   787 - 794   2006.4

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    DOI： 10.1111/j.1365-2672.2006.02864.x

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  • Presence of NADP+-specific glyceraldehyde-3-phosphate dehydrogenase and CcpA-dependent transcription of its gene in the ruminal bacterium Streptococcus bovis.

    Asanuma, N. and T. Hino.

    FEMS Microbiology Letters   257   17-23   2006.4

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  • Augmentation of vaccenate production and suppression of vaccenate biohydrogenation in cultures of mixed ruminal microbes

    S Fukuda, Y Suzuki, M Murai, N Asanuma, T Hino

    JOURNAL OF DAIRY SCIENCE   89 ( 3 )   1043 - 1051   2006.3

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  • Oral administration of Butyrivibrio fibrisolvens, a butyrate-producing bacterium, decreases the formation of aberrant crypt foci in the colon and rectum of mice

    S Ohkawara, H Furuya, K Nagashima, N Asanuma, T Hino

    JOURNAL OF NUTRITION   135 ( 12 )   2878 - 2883   2005.12

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  • Ability to utilize lactate and related enzymes of a ruminal bacterium, Selenomonas ruminantium.

    Asanuma, N. and T. Hino.

    Animal Science Journal   76 ( 4 )   345-352 - 352   2005.8

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  • Characterization and transcription of the genes involved in butyrate production in Butyrivibrio fibrisolvens TypeI and II strains.

    Asanuma, N., M. Ishiwata, T. Yoshii, M. Kikuchi, Y. Nishina and T. Hino.

    Current Microbiology   51   91-94   2005.7

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  • Nitrosoamine-forming activity of intestinal bacteria in dogs, and effect of nitrate- and nitrite-reducing bacteria on the suppression of nitrosation

    Yoshii T., Asanuma N., Furuhashi M., Hino T.

    Journal of Pet Animal Nutrition   8 ( 1 )   9-17 - 17   2005.4

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    Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society of Pet Animal Nutrition  

    In order to reduce nitrosoamine formation in the intestine of dogs, nitrosating activity of mixed intestinal microbes was examined with fecal samples collected from 30 dogs. The number of bacteria that have nitrosating activity tended to increase with the age of dogs irrespective of breed or sex. Incubation of dog feces with nitrite suggested that the growth of nitrosating bacteria and/or nitrosatingenzyme activity are enhanced by nitrite. However, since nitrosation was observed even when heat-killed fecal bacteria were incubated (non-enzymatic reaction), it is desirable to prevent the accumulation of secondary amines and nitrite in the intestines irrespective of whether or not intestinal bacteria are involved. Nitrate reductase in Escherichia coli has been reported to have nitrosating activity, but Selenomonas ruminantium and Wolinella succinogenes, nitrate- and nitritereducing ruminal bacteria, were shown to have little nitrosating activity. When mixed fecal bacteria from an old dog were grown with nitrite, addition of S. ruminantium stimulated nitrite reduction and decreased the nitrosating activity of the mixed fecal bacteria. These results suggest that a decrease in nitrite due to nitrite reduction by S. ruminantium brought about a decrease in nitrosatingenzyme activity. When mixed fecal bacteria were grown with nitrate, addition of W succinogenes that has a higher ratio of nitrite-reducing activity to nitrate-reducing activity than S. ruminantium, shortened the time during which nitrite was present. As a result, the nitrosating activity of the mixed bacteria decreased. Therefore, introduction of S. ruminantium and W succinogenes to the intestines may decrease nitrite accumulation or concentration, which may decrease nitrosoamine formation.

    DOI： 10.11266/jpan1998.8.1-2_9

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  • Application of Sphingomyelin to the Prevention of Colorectal Cancer in Dogs, and Utilization of Intestinal Bacteria to Maximize the Effect

    Furuya H., Ohkawara S., Asanuma N., Hino T.

    Journal of Pet Animal Nutrition   8 ( 1 )   30-39 - 39   2005.4

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    Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society of Pet Animal Nutrition  

    For the promotion of health, especially the prevention of colorectal cancer in dogs, preliminary experiments were performed to gain basic knowledge on the conditions to maximize the effect of sphingomyelin (SM) and the involvement of intestinal bacteria in SM degradation. Feeding a die t containing SM to mice decreased the number of aberrant crypt foci (ACF) in the colon and rectum induced by 1,2 ?aimethylhydrazine. Analysis of sphingolipids in the feces of mice and dogs sugge sted that considerably large amounts of endogenous SM and its degradation products enter the intestinal tract. However, the amounts appeared to be insufficient to prevent colorectal cancer, which implies that dietary supplementation of SM is important. To exert effects, SM needs to be degraded to ceramide, and more desirably, to sphingosin, but SM and ceramide did not appear to be readily degraded in the intestinal tract. In mice, removal of intestinal bacteria with antibiotics did not alter the amount of fecal SM, suggesting that mice have few or no bacteria that have sphingomyelinase (SMase). However, the amount of fecal sphingosin was significantly reduced by antibiotics, suggesting that mice have bacteria having ceramidase. Evidence was obtained for the first time to show the presence of intestinal bacteria having SMase and ceramidase in dogs, although the percentage of dogs that possess such bacteria was low. The amount of fecal SM was smaller, and that of sphingosin was larger, in dogs that had bacteria having SMase and ceramidase, which suggests that these bacteria contribute to the degradation of SM and ceramide in the intestinal tract. These results show that introduction of bacteria having these enzymes to dogs can enhance the effectiveness of SM given in the diet.

    DOI： 10.11266/jpan1998.8.1-2_30

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  • Preservation of viable Butyrivibrio fibrisolvens by freeze-drying to use as a probiotic for dogs and cats

    Ohkawara S., Furuya H., Asanuma N., Hino T.

    Journal of Pet Animal Nutrition   8 ( 1 )   1-8 - 8   2005.4

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    Aiming at using Butyrivibrio fibrisolvens as a probiotic for dogs and cats, optimal conditions of freeze-drying to keep cell viability were determined. Highest percentages of viable cells were obtained by freeze-drying below -40°C. Sucrose and fructooligosaccharide had a high protective effect against cell damages during freeze-drying. The percentage of viable cells was maximum when cells were freeze-dried at the cell density of 10<SUP>10</SUP> viable counts/ml. Refreeze-drying following the first freeze-drying enhanced the proportion of viable cells possibly because of complete dehydration. Approximately 80 % of cells were kept viable by freeze-drying under optimal conditions. Resistance of strains MDT-1, ATCC19171 and TH1 to damages during freeze-drying was higher than other strains of B. fibrisolvens, which indicates that these strains are suitable for probiotics in this context. When the freeze-dried cells of MDT-1 and TH1 were preserved at room temperature under air, more than 40 % of the cells were viable even after 3 months. When stored in a refrigerator (4°C) for 6 months, more than 80 % cells were viable. Little loss of viability was noted when stored in a freezer (-20°C) for 6 months, suggesting that the two strains can be kept viable for long periods of time. When freeze-dried cells of MDT-1 were grown in the presence of a small amount of oxygen, viable cells decreased. However, addition of Lactobacilus fermentum JCM1137 alleviated the loss of viability, suggesting that L. fermentum consumed oxygen. When B. fibrisolvens is utilized as a probiotic, it may be beneficial to use oxygen-consuming lactic acid bacteria simultaneously.

    DOI： 10.11266/jpan1998.8.1-2_1

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  • A new strain of Butyrivibrio fibrisolvens that has high ability to isomerize linoleic acid to conjugated linoleic acid

    S Fukuda, H Furuya, Y Suzuki, N Asanuma, T Hino

    JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY   51 ( 2 )   105 - 113   2005.4

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  • Effect of ethanol on nitrate and nitrite reduction and methanogenesis in the ruminai microbiota

    Takahiro Yoshii, Narito Asanuma, Tsuneo Hino

    Animal Science Journal   76 ( 1 )   37 - 42   2005.2

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    DOI： 10.1111/j.1740-0929.2005.00235.x

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  • Molecular characterization and transcription of the luxS gene that encodes LuxS autoinducer 2 synthase in Streptococcus bovis

    N Asanuma, T Yoshii, T Hino

    CURRENT MICROBIOLOGY   49 ( 5 )   366 - 371   2004.11

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    DOI： 10.1007/s00284-004-4356-x

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  • 乳酸利用の増強によるルーメンアシドーシスおよびメタン生成の抑制

    浅沼成人・日野常男

    日本畜産学会報   75   543-550   2004.11

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  • イヌの腸内微生物混合系における共役リノール酸生成に及ぼすButyrivibrio fibrisolvensとBifidobacterium breveの同時添加の効果

    福田真嗣・鈴木裕義・村井美希・浅沼成人・日野常男

    日本ペット栄養学会誌   7   115-122   2004.10

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  • イヌ・ネコの腸内細菌と健康

    日野常男・浅沼成人

    日本ペット栄養学会誌   7   139-152   2004.10

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  • Molecular characterization of CcpA and involvement of this protein in transcriptional regulation of lactate dehydrogenase and pyruvate formate-lyase in the ruminal bacterium Streptococcus bovis

    N Asanuma, T Yoshii, T Hino

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   70 ( 9 )   5244 - 5251   2004.9

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    DOI： 10.1128/AEM.70.9.5244-5251.2004

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  • ルーメンバクテリア, Butyrivibrio fibrisolvensの低pH耐性

    大河原壮・古谷英樹・浅沼成人・日野常男

    日本畜産学会報   75   387-393   2004.9

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  • Molecular characteristics of phosphoenolpyruvate: Mannose phosphotransferase system in Streptococcus bovis

    N Asanuma, T Yoshii, T Hino

    CURRENT MICROBIOLOGY   49 ( 1 )   4 - 9   2004.7

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    DOI： 10.1007/s00284-003-4232-0

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  • Effects of the overexpression of fructose-1,6-bisphosphate aldolase on fermentation pattern and transcription of the genes encoding lactate dehydrogenase and pyruvate formate-lyase in a ruminal bacterium, Streptococcus bovis

    N Asanuma, T Yoshii, M Kikuchi, T Hino

    JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY   50 ( 2 )   71 - 78   2004.4

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  • Molecular characterization and transcriptional regulation of nitrate reductase in a ruminal bacterium, Selenomonas ruminantium

    N Asanuma, M Iwamoto, T Yoshii, T Hino

    JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY   50 ( 2 )   55 - 63   2004.4

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  • サプリメントとしての利用を目的としたスフィンゴミエリンの調製法の改良

    古谷英樹・大河原壮・浅沼成人・日野常男

    明治大学農学部研究報告   138   1-10   2004.3

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  • ルーメンにおける硝酸・亜硝酸還元の増強によるメタン生成の抑制のためのWolinella succinogenesの活用

    浅沼成人・吉井貴宏・日野常男

    日本畜産学会報   75   45-52   2004.2

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  • Molecular characteristics and transcription of the gene encoding a multifunctional alcohol dehydrogenase in relation to the deactivation of pyruvate formate-lyase in the ruminal bacterium Streptococcus bovis

    N Asanuma, T Yoshii, T Hino

    ARCHIVES OF MICROBIOLOGY   181 ( 2 )   122 - 128   2004.2

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    DOI： 10.1007/s00203-003-0638-0

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  • Isolation and evaluation of nitrate-and nitrite-reducing Streptococcus bovis from cat feces

    Yoshii T., Asanuma N., Hino T.

    Journal of Pet Animal Nutrition   7 ( 1 )   15-23 - 23   2004.1

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    In order to augment lactate production and nitrite reduction simultaneously in the intestines of dogs and cats, a bacterium that has both lactate-producing and nitrite-reducing capacity was iso lated from cat feces. The isolate was identified as Streptococcus bovis. The isolated <I>S. bovis</I> wa found to belong to the biotype II that has no ill effect on the host. The specific activity (the amount of enzyme per cell mass) of nitrite reductase (NiR) increased when the isolate was grown in the presence of nitrite, suggesting that NiR synthesis is stimulated by nitrite. When mixed fecal bacteria from an old dog or cat were cultured in a growth medium containing resistant starch, addition of the isolate increased lactate production and lowered culture pH. When similar culture experiments were performed with a medium containing nitrite, addition of the isolate stimulated nitrite reduction. However, when nitrate was added to cultures, addition of the isolate increased nitrite accumulation. This result can be explained by the fact that the rate of nitrate reduction by the isolate is higher than that of nitrite reduction. Therefore, the isolate needs to be so improved that NiR synthesis is enhanced. Use of the improved strain as a probiotic would increase lactate production and decrease nitrite accumulation in the intestines.

    DOI： 10.11266/jpan1998.7.1_15

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  • Number of nitrate- and nitrite-reducing Selenomonas ruminantium in the rumen, and possible factors affecting its growth

    Takahiro Yoshii, Narito Asanuma, Tsuneo Hino

    Animal Science Journal   74 ( 6 )   483 - 491   2003.12

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    DOI： 10.1046/j.1344-3941.2003.00142.x

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  • 亜硝酸還元能の高い新規な菌の単離とその導入によるルーメン内における亜硝酸還元の増強

    浅沼成人・吉井貴宏・日野常男

    明治大学農学部研究報告   137   1-17   2003.12

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  • Characterization and transcription of the genes encoding enzymes involved in butyrate production in Butyrivibrio fibrisolvens.

    Asanuma, N., M. Kawato, S. Ohkawara and T. Hino.

    Current Microbiology   47   203-207   2003.10

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  • Effect of nitrite on the populations of cat intestinal flora, and isolation of nitrite-reducing bacteria from cat feces

    Yoshii T., Asanuma N., Hino T.

    Journal of Pet Animal Nutrition   6 ( 3 )   121-130 - 130   2003.10

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    Effect of nitrite on the populations of cat intestinal flora was examined. When cat faces were incubated in a growth medium containing glucose, maltose, and, cellobiose, addition of 3 mM nitrite increased lactate production, and decreased the production of propionate, formate, and butyrate. This suggests that nitrite affects the composition of intestinal flora. RFLP (restriction fragment length polymorphism) analysis also showed that addition of nitrite altered the composition of intestinal flora. In the presence of nitrite, the proportions of <I>Enterococcus</I> sp. and Clostridium sp. increased. Then, nitrite-reducing bacteria were isolated from cat feces by successive cultivation in a medium containing nitrite. Based on Gram-staining, shape of cell, characteristics of colony, growth conditions, sequence of 16S rDNA, utilization of carbohydrates, and fermentation products, the isolated bacteria were putatively identified as <I>Clostridium perfringens, Escherichia coli</I>, and Streptococcus bovis. However, putative S. bovis had extremely low nitrite-reducing ability and little nitrite reductase activity. Putative <I>C. perfringens</I> and <I>E. coil</I> had much higher nitrite-reducing ability and nitrite reductase activity. These two bacteria were found at high frequency, which agreed with the result of RFLP analysis. These results suggest that the two bacteria are predominant nitritereducing bacteria in the cat intestine. The amount of nitrite reductase in cells, or ability to reduce nitrite, was different among the strains even within the same species.

    DOI： 10.11266/jpan1998.6.3_121

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  • イヌの腸管内の酪酸生成菌

    浅沼成人・日野常男

    日本ペット栄養学会誌   6   53-58   2003.4

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  • Regulator protein that potentially triggers the transcription of the H+-ATPase operon in a ruminal bacterium, Streptococcus bovis.

    Ohkawara, S., N. Asanuma and T. Hino.

    Animal Science Journal   74   153-156   2003.3

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  • Molecular characterization of HPr and related enzymes, and regulation of HPr phosphorylation in the ruminal bacterium Streptococcus bovis.

    Asanuma, N. and T. Hino.

    Archives of Microbiology   179   205-213 - 213   2003.3

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  • ルーメンバクテリアの低pH耐性と細胞膜脂質との関係

    大河原壮・浅沼成人・日野常男

    明治大学農学部研究報告   134   1-17   2003.3

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  • Nitrate and nitrite reduction by intestinal microbes of dogs and cats

    Yoshii T., Asanuma N., Abe T., Iwamoto M., Hino T.

    Journal of Pet Animal Nutrition   6 ( 1 )   1-6 - 6   2003.1

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    Because presence of nitrite in the intestines of animals can be a risk factor of carcinogenesis, nitrate and nitrite reduction by the intestinal microbes of dogs and cats was examined by using fecal samples. Incubation of dog and cat feces with nitrate indicated that all the animals examined had nitrate- and nitrite-reducing bacteria in their intestines. The ratio of nitrite reduction rate to nitr ate reduction rate was found to decrease with the ages of dogs and cats, suggesting that nitrite can be accumulated in senior animals. Nitrite reductase activity in mixed fecal microbes increased with an increase in nitrite concentration in the medium, suggesting that the synthesis of nitrite reductase is enhanced by nitrite. The optimal pH of nitrate and nitrite reduction by mixed fecal microbes was 7.0, and in a pH range of 6.0-7.2 nitrate reduction was faster than nitrite reduction, suggesting that nitrite can be accumulated within this pH range. Because nitrate reduction rate was low at a pH value below 6.0, it is desirable to keep the pH of large intestines around 6.0. When formate was added as an electron donor to cultures of mixed fecal microbes, the ratio of nitrite reduction rate to nitrate reduction rate was enhanced, suggesting that nitrite accumulation in the intestines can be prevented by increasing formate production by intestinal bacteria.

    DOI： 10.11266/jpan1998.6.1_1

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  • Suppression of ruminal methanogenesis by decreasing the substrates available to methanogenic bacteria.

    Hino, T. and N. Asanuma.

    Nutrition Abstracts and Reviews Series B   73   1-8 - 8   2003.1

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  • Production of conjugated linoleic acid by intestinal bacteria in dogs and cats

    S Fukuda, N Ninomiya, N Asanuma, T Hino

    JOURNAL OF VETERINARY MEDICAL SCIENCE   64 ( 11 )   987 - 992   2002.11

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  • Regulation of fermentation in a ruminal bacterium, Streptococcus bovis, with special reference to rumen acidosis.

    Asanuma, N. and T. Hino.

    Animal Science Journal   73   313-325 - 325   2002.10

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  • Suppression of ruminal methanogenesis by decreasing the substrates available to methanogenic bacteria.

    Hino, T. and N. Asanuma.

    Nutrition Abstracts and Reviews Series B   73   1-8   2002.9

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  • Ability of Selenomonas ruminantium, Veillonella parvula, and Wolinella succinogenes to reduce nitrate and nitrite with special reference to the suppression of ruminal methanogenesis

    M Iwamoto, N Asanuma, T Hino

    ANAEROBE   8 ( 4 )   209 - 215   2002.8

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    DOI： 10.1006/anae.2002.0428

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  • Molecular characterization and expression of pyruvate formate-lyase-activating enzyme in a ruminal bacterium, Streptococcus bovis.

    Asanuma, N. and T. Hino.

    Applied and Environmental Microbiology   68   3352-3357 - 3357   2002.7

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  • Numbers of nitrate-reducing bacteria in the rumen as estimated by competitive PCR.

    Asanuma, N., M. Iwamoto, M. Kawato and T. Hino.

    Animal Science Journal   73   199-205   2002.6

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  • Isolation of Conjugated Linoleic Acid-Producing Bacteria from Dog and Cat Feces

    Fukuda S., Ninomiya N., Asanuma N., Hino T.

    Journal of Pet Animal Nutrition   5 ( 2 )   70-78 - 78   2002.4

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    Three bacteria producing conjugated linoleic acid (CLA) were isolated from dog and cat feces. The first isolate was presumed to be a species of <I>Streptococcus</I> or <I>Lactococcus</I>, and the second isolate was probably <I>Eubacterium aerofaciens</I>. These two bacteria have not been known as CLA-producing bacteria. The third isolate was identified as a type II strain of <I>Butyrivibrio fibrisolvens</I>. Of the three isolates, <I>B. fibrisolvens</I> was found to have the highest capacity to produce CLA. To increase CLA production in the large intestine, it may be effective to increase the cell number of this strain of <I>B. fibrisolvens</I>. Addition of the isolated strain of <I>B. fibrisolvens</I> to a culture of mixed fecal microorganisms markedly increased CLA production. Experiments with six different strains of B. fibrisolvens indicated that the rates of linoleic acid (LA) isomerization and CLA reduction are correlated to the amounts of LA isomerase and CLA reductase in cells. These results suggest that CLA production, oraccumulation, can be greatly increased by enhancing the synthesis of LA isomerase and suppressing the synthesis of CLA reductase.

    DOI： 10.11266/jpan1998.5.2_70

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  • Fructose bisphosphate aldolase activity and glycolytic intermediate concentrations in relation to lactate production in Streptococcus bovis.

    Asanuma, N. and T. Hino.

    Anaerobe   8   1-8 - 8   2002.2

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  • イヌの腸内菌叢におけるButyrivibrio fibrisolvensの菌数の増加

    浅沼 成人, 河戸 勝, 吉井 貴宏, 日野 常男

    ペット栄養学会誌   5 ( Supplement )   29 - 30   2002

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    酪酸および共役リノール酸生成能の高い細菌,<I>Butyrivibrio fibrisolvens</I>をイヌの糞中混合微生物系に添加し,どの多糖類をエネルギー源とした場合にその菌数が増加するかを調べた。限界デキストリンまたはペクチンを基質として培養した場合には,24時間後の総菌数に対する<I>B.fibrisolvens</I>の菌数の割合は増加しなかったが,キシランを基質とした場合には,<I>B.fibrisolvens</I>の添加により総菌数に対する<I>B.fibrisolvens</I>の菌数の割合が増加し,酪酸生成も増加した。従って,餌中のキシラン含量を増加させれば,大腸内の<I>B.fibrisolvens</I>の数を増加させることができると思われた。

    DOI： 10.11266/jpan1998.5.supplement_29

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  • Presence of Butyrivibrio fibrisolvens in the digestive tract of dogs and cats, and its contribution to butyrate production

    N Asanuma, M Kawato, T Hino

    JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY   47 ( 6 )   313 - 319   2001.12

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  • Effects of energy substrates on nitrate reduction and nitrate reductase activity in a ruminal bacterium, Selenomonas ruminantium

    M Iwamoto, N Asanuma, T Hino

    ANAEROBE   7 ( 6 )   315 - 321   2001.12

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    DOI： 10.1006/anae.2001.0397

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  • ルーメン微生物における硝酸・亜硝酸還元に及ぼすプロトゾアの影響

    岩本美和・浅沼成人・日野常男

    関東畜産学会報   51   9-15 - 15   2001.3

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  • Molecular characterization, enzyme properties and transcriptional regulation of phosphoenolpyruvate carboxykinase and pyruvate kinase in a ruminal bacterium, Selenomonas ruminantium

    N Asanuma, T Hino

    MICROBIOLOGY-UK   147   681 - 690   2001.3

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  • Effect of pH and electron donors on nitrate and nitrite reduction in ruminal microbiota.

    Iwamoto, M., N. Asanuma and T. Hino.

    Animal Science Journal   72   117-125   2001.3

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  • Effects of pH and energy supply on activity and amount of pyruvate formate-lyase in Streptococcus bovis

    N Asanuma, T Hino

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   66 ( 9 )   3773 - 3777   2000.9

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  • Activity and properties of fumarate reductase in ruminal bacteria

    N Asanuma, T Hino

    JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY   46 ( 3 )   119 - 125   2000.6

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  • Effect of Addition of Cellulase Preparations on Fiber Digestion in Beef Cattle

    HINO Tsuneo, MIWA Takehiro, ASANUMA Narito, SHIRAISHI Kuniko, KITAMURA Hiroshi, MIZOGUCHI Hideki

    Nihon Chikusan Gakkaiho   71 ( 7 )   J46-J50 - 50   2000.2

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    To examine whether addition of cellulase preparations enhances fiber digestion in cattle, in vitro culture experiments with ruminal microbiota and in vivo digestion trials were conducted. Two cellulase preparations with an optimal pH of 4-5 and 6-7 were subjected to this study. Little feed-NDF was digested by ruminal microbiota incubated at pH 5.5-6.0, and addition of cellulase preparations markedly enhanced NDF digestion. Also at pH6.5-7.0, cellulase preparations significantly increased NDF digestibility, but the effect was smaller because cellulase preparations with low optimal pH were used. In each case, the effect was enhanced by the combination of two cellulase preparations, and the greatest effect was noted when three preparations were combined. Addition of three preparations to cattle fed high-concentrate feed markedly enhanced NDF digestibility. Digestion of hay-NDF and cellulose powder in nylon bags suspended in the rumen was also greatly enhanced by cellulase preparations. Since ruminal pH was always 5-6 in these cattle, cellulase preparations with the optimal pH of 4-5 probably exerted a great effect. When three preparations were added to cattle fed high-roughage feed, NDF digestion was significantly improved, but again the effect was smaller due to the use of cellulase preparations with low optimal pH. These results indicate that addition of cellulase preparations to cattle feed improves fiber digestion, especially in cattle fed high-concentrate feed.

    DOI： 10.2508/chikusan.71.7_46

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  • イヌおよびネコの腸管内における酪酸生成菌,Butyrivibrio fibrisolvens の存在

    浅沼 成人, 河戸 勝, 日野 常男

    ペット栄養学会誌   3 ( Supplement )   12 - 13   2000

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    イヌ・ネコの腸管内の酪酸生成に着目し,種々の観点から重要な酪酸生成菌と考えられるButyrivibrio fibrisolvens の存在の有無および菌数について調べた。即ち,ペットフードを供試した成イヌおよび成ネコの糞中の菌数を競合PCRにより測定した。乳酸生成量に基づいて分類されている<I>B.fibrisolvens</I>の2つのグループのうち,乳酸生成の少ないグループの株は検出できなかった。しかし,乳酸生成の多いグループの菌株の標的DNAは糞中総DNAの5x10<SUP>-4</SUP>および3x10<SUP>-4</SUP>のレベルで検出された。加熱した糞サンプルに<I>B.fibrisolvens</I>をもともと存在していた菌数と等しくなるように添加して培養した場合の酪酸生成量は非加熱の糞サンプルを培養した場合の酪酸生成量の30%であった。従って,イヌ・ネコの腸管内における酪酸生成は,<I>B.fibrisolvens</I>よりもそれ以外の菌によるところが大きいと考えられる。

    DOI： 10.11266/jpan1998.3.supplement_12

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  • Effect of the addition of fumarate on methane production by ruminal microorganisms in vitro

    N Asanuma, M Iwamoto, T Hino

    JOURNAL OF DAIRY SCIENCE   82 ( 4 )   780 - 787   1999.4

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  • The production of formate, a substrate for methanogenesis, from compounds related with the glyoxylate cycle by mixed ruminal microbes.

    Asanuma N, M. Iwamoto and T. Hino.

    Animal Science Journal   70 ( 2 )   67-73 - 73   1999.3

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  • Structure and transcriptional regulation of the gene encoding pyruvate formate-lyase of a ruminal bacterium, Streptococcus bovis

    N Asanuma, M Iwamoto, T Hino

    MICROBIOLOGY-UK   145   151 - 157   1999.1

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  • Formate metabolism by ruminal microorganisms in relation to methanogenesis.

    Asanuma N, M. Iwamoto and T. Hino.

    Animal Science and Technology   69 ( 6 )   576-584 - 584   1998.6

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  • Regulation of lactate dehydrogenase synthesis in a ruminal bacterium, Streptococcus bovis

    N Asanuma, M Iwamoto, T Hino

    JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY   43 ( 6 )   325 - 331   1997.12

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  • Tolerance to low pH and lactate production in rumen bacteria.

    Asanuma, N. and T. Hino.

    Animal Science and Technology   68   367-376 - 376   1997.4

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  • Effect of methane production on plant fiber digestion by rumen microorganisms.

    Hino, T., T. Miwa, H. Saitoh, H. Esaki, S. Takagi, N. Asanuma, and J. Umemori.

    Bulletin of School of Agriculture Meiji University   106   27-33   1995.12

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Books

  • ルーメン微生物の発酵調節とエネルギー代謝の調節

    浅沼成人・日野常男（ Role： Joint author）

    「新ルーメンの世界」板橋久雄 編集 農文協  2004.2 

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    Responsible for pages：453-532   Language：Japanese   Book type：Scholarly book

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  • "Understanding metabolic regulation in the ruminal bacteria, Streptococcus bovis, Selenomonas ruminantium, and Megasphaera elsdenii. no."

    "Asanuma, N. and T. Hino"（ Role： Joint author）

    Gastrointestinal Microbiology in Animals  2002.9 

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    Responsible for pages：61-87   Language：English   Book type：Scholarly book

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Presentations

  • 新規グルコシルセラミド水解菌のプロバイオテイクス利用に向けた特性の解析

    湯山星加, 浅沼成人

    関東畜産学会第74回大会  2019.11 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：茨城県 筑波市  

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  • 共役リノール酸を生成する細菌Butyrivinrio fibrisolvensのプロバイオティクス特性

    鈴木慎人, 浅沼成人

    関東畜産学会第74回大会  2019.11 

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    Venue：茨城県 筑波市  

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  • 一般家庭で飼育されているイヌにおけるグルコシルセラミド水解菌の分布

    高橋宏宣, 湯山星加, 浅沼成人

    関東畜産学会第74回大会  2019.11 

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    Venue：茨城県 筑波市  

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  • 植物由来のセラミドの摂取による実験的大腸炎の緩和

    浅沼 成人

    第12回セラミド研究会学術集会  2019.10 

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    Venue：北海道 札幌市  

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  • The utilization of probiotics and probiotics to augment the digestion of glucosylceramide contained in plant foods International conference

    Yuyama S., Asanuma N.

    19th International Nutrition & Diagnostics Conference -INDC2019  2019.10 

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    Venue：Czech Republic Prague  

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  • The presence of the glucosylceramide-hydrolyzing bacteria in pet dogs International conference

    Takahashi H., Yuyama S., Asanuma N.

    19th International Nutrition & Diagnostics Conference -INDC2019  2019.10 

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    Venue：Czech Republic Prague  

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  • 腸内細菌によるグルコシルセラミドの分解 Invited

    浅沼 成人

    第71回日本生物工学会  2019.9 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：岡山県岡山市  

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  • 腸内細菌を用いた食事性グルコシルセラミドの有効利用 Invited

    浅沼 成人

    第24回国際食品素材/添加物展・会議  2019.5 

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    Venue：東京  

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  • Metabolic pathway of conjugated fatty acids in newly isolated bacterium from dog feces International conference

    S. Nobukawa and N. Asanuma

    The Food Factor conference 2018  2018.11 

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    Venue：Spain Malaga  

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  • Probiotic utilization of Glucerabacter canisensis for augmenting the digestion of glucosylceramide from plant foodstuffs International conference

    N. Kawahara and N. Asanuma

    The Food Factor conference 2018  2018.11 

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    Venue：Spain Malaga  

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  • 腸内細菌によるグルコシルセラミドの水解とそのプロバイオティクス利用 Invited

    浅沼 成人

    第11回セラミド研究会学術集会  2018.10 

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    Venue：東京  

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  • The population of the glucosylceramide-hydrolyzing bacterium, Glucerabacter canisensis, in dogs of different age groups International conference

    M. Kawata and N. Asanuma

    18th International Nutrition & Diagnostics Conference -INDC2018  2018.9 

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    Venue：Czech Republic Prague  

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  • Properties of a novel strain of Butyrivibrio fibrisolvens that has high ability to produce conjugated linoleic acid International conference

    M. Suzuki, K. Ii, and N. Asanuma

    18th International Nutrition & Diagnostics Conference -INDC2018  2018.9 

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    Venue：Czech Republic Prague  

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  • Fructooligosaccharide supporting the growth of glucosylceramide-hydrolyzing bacterium in cultures of mixed intestinal bacteria from mice International conference

    S. Yuyama and N. Asanuma

    18th International Nutrition & Diagnostics Conference -INDC2018  2018.9 

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    Venue：Czech Republic Prague  

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  • Effects of conjugated fatty acids produced by intestinal bacteria on skin whitening International conference

    S. Akutsu and N. Asanuma

    18th International Nutrition & Diagnostics Conference -INDC2018  2018.9 

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    Venue：Czech Republic Prague  

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  • Transition of the intestinal glucosylceramide-hydrolyzing bacteria in dogs with age International conference

    Asanuma N

    VIII International Conference on Enviromental, Industrial and Applied Microbiology  2018.5 

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    Language：English   Presentation type：Poster presentation  

    Venue：Spain Torremolinos  

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  • Enzyme properties of glucosylceramidase in the novel bacterium A that is a member of the Clostridium coccoides group International conference

    Tsukamoto A, Kawata M, Asanuma N

    VII International Conference on Enviromental, Industrial and Applied Microbiology  2017.10 

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    Venue：Spain Madrid  

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  • Effects of lipid metabolites by dog fecal bacteria on skin whitening International conference

    Akutsu S, Asanuma N

    VII International Conference on Enviromental, Industrial and Applied Microbiology  2017.10 

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    Venue：Spain Madrid  

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  • Comparison of the ability to isomerize linoleic acid to conjugated linoleic acid among newly isolated Butyrivibrio sp. International conference

    Nobukawa S, Suzuki M, Asanuma N

    VII International Conference on Enviromental, Industrial and Applied Microbiology  2017.10 

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    Venue：Spain Madrid  

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  • Characterizations of newly isolated Clostridium coccoides group bacterium as its probiotic effects International conference

    Kawata M, Tsukamoto A, Asanuma N

    VII International Conference on Enviromental, Industrial and Applied Microbiology  2017.10 

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    Venue：Spain Madrid  

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  • Probiotic utilization of the newly isolated Butyrivibrio sp. improves the skin barrier function International conference

    Kawahara N, Asanuma N

    VII International Conference on Enviromental, Industrial and Applied Microbiology  2017.10 

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    Venue：Spain Madrid  

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  • Microbial populations related to conjugated linoleic acid production in dogs International conference

    Suzuki M, Nobukawa S, Asanuma N

    VII International Conference on Enviromental, Industrial and Applied Microbiology  2017.10 

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    Venue：Spain Madrid  

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  • Augmentation of the hydrolysis of dietary glucosylceramide in the large intestine by a newly bacterium isolated from canine feces International conference

    Asanuma N

    FEMS 2017 7th Congress of European Microbiologists  2017.7 

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    Language：English   Presentation type：Poster presentation  

    Venue：Spain Valencia  

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  • Characterizations of plant glucosylceramide hydrolyzing bacteria isolated from canine feces International conference

    Kawata M, Tsukamoto A, Asanuma N

    16th International Nutrition & Diagnostics Conference -INDC2016  2016.10 

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    Venue：Czech Republic Prague  

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  • Availability of dog feces as the amino acid source for Butyrivibrio fibrisolvens International conference

    Kawahara N, Akutsu S, Asanuma N

    16th International Nutrition & Diagnostics Conference -INDC2016  2016.10 

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    Venue：Czech Republic Prague  

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  • Augmentation of the utilization of dietary glucosylcermide by the novel Bacterium A International conference

    Tsukamoto A, Kawata M, Asanuma N

    16th International Nutrition & Diagnostics Conference -INDC2016  2016.10 

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    Venue：Czech Republic Prague  

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  • Inhibition of Melanogenesis by Plant CeramideE in B16 Melanoma Cells International conference

    Akutsu S, Kawahara N, Asanuma N

    16th International Nutrition & Diagnostics Conference -INDC2016  2016.10 

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    Venue：Czech Republic Prague  

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  • Application of plant ceramide as antibacterial agent to prevent Escherichia coli O157:H7 infection International conference

    Asanuma N

    IV International Conference on Antimicrobial Research  2016.6 

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    Language：English   Presentation type：Poster presentation  

    Venue：Spain Torremolinos  

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  • Immunomodulatory effects of lipopolysaccharide from the Butyrivibrio group bacterium isolated from the goat rumen International conference

    Asanuma N

    15th International Nutrition & Diagnostics Conference -INDC2015  2015.10 

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    Venue：Czech Republic Prague  

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  • Effects of oral administration of the Butyrivibrio group bacterium on the inflammatory dry skin condition in chronic irritant contact dermatitis International conference

    Asanuma N

    FEMS 2015 6th Congress of European Microbiologists  2015.6 

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    Language：English   Presentation type：Poster presentation  

    Venue：Netherlands Maastricht  

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  • Probiotic utilization of the novel glucosylceramide-hydrolyzing bacterium to the alleviation of inflammatory bowel disease International conference

    Asanuma N

    14th International Nutrition & Diagnostics Conference -INDC2014  2014.9 

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    Venue：Czech Republic Prague  

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  • Probiotic Effects of the Newly Ceramide-Producing Bacterium with Dietary Supplementation of Glucosylcermide International conference

    Asanuma N, Tsukamoto A, Yamaya M

    International Scientific Conference Probiotics and Prebiotics 2014  2014.6 

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    Language：English   Presentation type：Poster presentation  

    Venue：Hungary Budapest  

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  • Application of Plant Ceramide to Modulate Intestinal Microflora International conference

    Yokoyama S, Asanuma N

    International Scientific Conference Probiotics and Prebiotics 2014  2014.6 

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    Language：English   Presentation type：Poster presentation  

    Venue：Hungary Budapest  

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  • Enhancement of the utilization of bioactive glucosylcermide by the newly strain isolated from dog feces International conference

    Asanuma N

    V International Conference on Enviromental, Industrial and Applied Microbiology  2013.10 

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    Venue：Spain Madrid  

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  • Molecular characterizations of a novel bacterium hydrolyzing plant glucosylceramide to ceramide International conference

    Asanuma N

    FEMS 2013 5th Congress of European Microbiologists  2013.7 

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    Language：English   Presentation type：Poster presentation  

    Venue：Deutschland Leipzig  

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  • Effects of corn ceramide on growth of mixed microflora and on experimental enterocolitis in mice International conference

    Asanuma N

    2013 Congress on gastrointestinal function meeting program  2013.4 

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    Language：English   Presentation type：Poster presentation  

    Venue：USA Chicago  

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  • Application of plant ceramide as antibacterial agent to modulate intestinal microflora and to prevent Clostridial infection International conference

    Asanuma N

    OIE Global Conference on the “Responsible and Prudent Use of Antimicrobial Agents for Animals”  2013.3 

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    Language：Japanese   Presentation type：Poster presentation  

    Venue：France Paris  

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  • Effectiveness of corn ceramide in manipulating gut microbiota in mouse with inflammatory bowel disease International conference

    II International Conference on Antimicrobial Research  2012.11 

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    Venue：Portuguese Republic Lisbon  

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  • Effects of ceramide produced from plant glucosylceramide to the alleviation of inflammatory bowel disease and gut microflora International conference

    Asanuma N

    Alternatives to Antibiotics  2012.9 

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    Language：English   Presentation type：Poster presentation  

    Venue：France Paris  

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  • Properties of a novel strain hydrolyzing plant glucosylceramide to ceramide International conference

    8th Joint Symposium INRA-Rowett on Gut Microbiology  2012.6 

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    Venue：France Clermont Ferrand  

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Awards

  • 日本畜産学会奨励賞

    2003  

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    Country/Region：Japan

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Research Projects

  • Augmentation of dietary sphingolipids utilization with the novel intestinal bacterium and its application to the alleviation or prevention of colon diseases

    Grant number：26450104  2014.4 - 2017.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Asanuma Narito

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    Authorship：Principal investigator 

    Grant amount：\5070000 （ Direct Cost: \3900000 、 Indirect Cost：\1170000 ）

    A novel anaerobic bacterium capable of hydrolyzing plant glucosylceramide (GluCer) was isolated from canine feces. This isolate hydrolyzed GluCer to ceramide without producing sphingoids at a higher rate than other bacteria. This enables the production of large amounts of ceramide. Oral administration of the isolate to mice fed diets supplemented with GluCer enhanced GluCer digestion without showing acute toxicity. Oral administration of the isolate with dietary GluCer to dextran sodium sulfate (DSS)-treated mice (inflammatory bowel disease model) alleviated the symptoms of colitis including body weight loss, diarrhea, and bloody stool. Myeloperoxidase (MPO) activity levels in colonic tissue were increased greatly in response to DSS, but probiotic utilization of this isolate lowered MPO activity significantly. Thus, this novel isolate might be used as a probiotic to augment the bioactive effects of dietary GluCer without the adverse effects.

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  • Molecular characterization of ruminal microbes for the augmentation of nitrite reduction and for the supplession of methane emission

    Grant number：23580377  2011.4 - 2014.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    ASANUMA Narito

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    Authorship：Principal investigator 

    Grant amount：\5200000 （ Direct Cost: \4000000 、 Indirect Cost：\1200000 ）

    The effects of dietary nitrate addition on ruminal fermentation characteristics and microbial populations in goats were investigated. Addition of nitrate resulted in a decrease in the total concentration of ruminal volatile fatty acids, an increase in the acetate:propionate ratio, and increases in the ammonia and lactate concentrations. Populations of methanogens, protozoa, and fungi were greatly decreased as a result of nitrate inclusion in the diet. There was modest or little impact of nitrate on the populations of prevailing species or genus of bacteria in the rumen, whereas Selenomonas ruminantium greatly increased. Quantification of the genes encoding nitrate reductase and nitrite reductase of S. ruminantium showed that these genes were increased by feeding nitrate, suggesting that the growth of nitrate- and nitrite-reducing S. ruminantium is stimulated by nitrate addition. Thus, S. ruminantium is likely to play a major role in nitrate and nitrite reduction in the rumen.

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  • Prevention of rumen acidosis by the modification of quorum sensing in ruminal bacteria

    Grant number：20780196  2008 - 2009

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (B)

    ASANUMA Narito

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    Grant amount：\4290000 （ Direct Cost: \3300000 、 Indirect Cost：\990000 ）

    It is desirable to consider a new strategy to prevent rumen acidosis, which is cased when ruminants are fed high-concentrate diets, because the dose of antibiotics and ionophores may cause the risk of residual antibiotics in animal products. Therefore, to prevent the overgrowth of the bacterium causing rumen acidosis (Streptococcus bovis), I identified a chemical signaling molecule to control the growth, which may be related to the change in the expression of several proteins, in S. bovis. This result may contribute to the development of a new strategy for the control of S. bovis overgrowth and the prevention of rumen acidosis.

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  • ルーメン細菌における代謝調節機構の解明とその制御

    Grant number：18780205  2006 - 2007

    日本学術振興会  科学研究費助成事業  若手研究(B)

    浅沼 成人

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    Grant amount：\3600000 （ Direct Cost: \3600000 ）

    Streptococcus bovisはルーメン内の主要な乳酸生成菌であり、デンプン分解能が高い。常在ルーメン菌の中では最も低pHに耐性のある菌であり、ルーメンアシドーシスを加速度的に進行させる菌と考えられている。乳酸生成を制御することを終局的目的として、S.bovisの発酵調節機構を解明するための実験を行なった。
    解糖系の鍵酵素の一つであるピルビン酸キナーゼ(PYK)に着目し、その酵素学的特性について調べた。PYKは、フルクトース1,6-ビスリン酸よりもグルコース6-リン酸(G6P)により大きく活性化された。ホスホエノールピルビン酸に対するPYKの親和性は、G6P濃度の影響をほとんど受けなかった。PYKは種々のヌクレオシド二リン酸をリン酸受容体として利用することができたので、菌体内ではATPだけでなく、GTP、ITP、UTPなどの生成にもPYKが利用されると考えられた。PYK活性は無機リン酸イオンによって大きく阻害されたが、その阻害はG6Pの添加により回復した。グルコースを基質とする培地でS.bovisをバッチ法で培養したところ、対数増殖後期以降、増殖速度が低下していくにつれて、菌体内のリン酸イオン濃度が増加し、G6P濃度が減少した。一方、PYK比活性、すなわち菌体量あたりのPYK量は、大きくは変化しなかった。従って、菌体内のPYK活性は、酵素量よりも菌体内のリン酸イオンとG6Pの濃度に大きく依存すると思われる。また、PYK活性は発酵パターンや増殖に影響すると推測された。
    また、解糖系の酵素の一つであるグリセルアルデヒド-3-リン酸脱水素酵素(GAPDH)の塩基配列を決定し、Genbankに登録した。リコンビナントGAPDHが活性を持つことを確認した。gapA過剰発現株および親株をグルコース培地で培養したところ、これらの菌株間で増殖速度には、ほとんど差がみられなかった。また、gapA過剰発現株の乳酸とギ酸の生成割合も親株の場合と違いがなかった。従って、GAPDHは律速酵素ではなく、その過剰発現が発酵パターンに及ぼす影響は小さいと考えられた。

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  • ルーメン細菌の代謝制御によるルーメンアシドーシスの防御および発酵の改善

    Grant number：16780190  2004 - 2005

    日本学術振興会  科学研究費助成事業  若手研究(B)

    浅沼 成人

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    Grant amount：\3500000 （ Direct Cost: \3500000 ）

    ルーメンバクテリア、Streptococcus bovisにおけるオートインデューサー合成系酵素遺伝子luxSの解析とその転写調節
    S.bovisは濃厚飼料給与時にルーメン内で急増し、乳酸生成を増加させてルーメンアシドーシスを引き起こすことがあるので、本菌の過増殖を抑えることが望ましい。細胞密度感知分子であるオートインデューサー2(AI-2)は、種々の菌から検出されており、菌細胞間の情報伝達を行なっていると考えられている。AI-2は、種々の遺伝子の発現に関与し、増殖を制御することも知られている。そこで、S.bovisにおけるAI-2の役割を明らかにするために、AI-2合成系の中心的な酵素であるLuxS AI synthaseをコードするluxS遺伝子の転写と増殖の関係について調べた。S.bovisのluxS遺伝子は486bpであり、単シストロン様式に転写された。luxS遺伝子の転写開始部位は1箇所であった。LuxS AI synthaseの推定アミノ酸配列は、他のStreptococcus属のものと高い相同性を示した。菌体内のluxS-mRNA量は対数増殖初期に大きく増加し、対数増殖中期以降には減少した。菌細胞へのグルコースの供給、または、増殖速度が低下する前にluxS-mRNA量は大きく低下した。luxSの転写は菌細胞密度とは直接は相関しておらず、菌を最大増殖速度に維持した場合にはluxS-mRNA量は高いままであった。従って、S.bovisにおけるAI-2活性は培養条件に応じて菌の生理状態や代謝を調節するためのシグナルとして作用する可能性がある。今後は、本菌におけるLuxS AI synthaseの役割や、AI-2合成の調節について解析する必要があろう。

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  • ルーメン微生物の発酵調節の機構

    Grant number：01J04108  2001 - 2003

    日本学術振興会  科学研究費助成事業  特別研究員奨励費

    浅沼 成人

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    Grant amount：\2400000 （ Direct Cost: \2400000 ）

    競合PCR法によって見積もったルーメン内における硝酸還元菌の存在数
    主要なルーメン菌の中で硝酸・亜硝酸還元能をもつことが知られているSelenomonas ruminantium、Veillonella parvula、およびWolinella succinogenesがヤギのルーメン内にどれ位の数で存在するかを調べた。これらの3菌の中でS.ruminantiumが最も多数存在し、V.parvula、およびW.succinogenesの数は非常に少なかった。しかし、後者の2菌の数は高濃厚飼料(低硝酸含量)を給与した場合よりも、高粗飼料(高硝酸含量)を給与した場合の方が多い傾向があった。そこで、高濃厚飼料に硝酸を添加したところ、両菌の数は明らかに増加した。従って、これら2菌の数は硝酸の増加によって増加すると考えられた。これらの硝酸還元菌の数を更に増やすことができれば、硝酸還元反応が増加し、発酵を抑制せずにメタン生成を抑制できると考えられた。
    Streptococcus bovisのPFL活性化酵素の遺伝子解析と代謝的意義
    ルーメンアシドーシスの進行に大きく関与するS.bovisにおける乳酸生成の制御について検討した。本菌の乳酸生成はピルビン酸・ギ酸開裂酵素(PFL)の活性の影響を受けるので、PFLを活性化するPFL活性化酵素(PFL-AE)について解析した。PFL-AE遺伝子の塩基配列を決定し、本酵素の合成が転写レベルで調節されていることを示した。また、精製したPFL-AEを用いてその特性を調べ、Fe^<2+>を補因子として要求することを示した。S.bovisにPFL-AEの遺伝子を導入して過剰発現させて、乳酸とギ酸の生成割合は親株の場合と違いがなく、また活性型PFLの量にも違いがなかったので、S.bovisの菌体内にはPFLの活性化に十分な量のPFL-AEが常に存在すると考えられた。つまり、PFL活性はPFLの量と電子の供与速度によって左右されると考えられた。

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  • 消化管微生物の遺伝子的代謝調節機構の解析

    2001

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