Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1126/science.1257800

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Language：Japanese   Publisher：一般社団法人 日本土壌肥料学会  

DOI： 10.20710/dohikouen.69.0_56_3

CiNii Research

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Language：English   Publishing type：Research paper (scientific journal)  

An Arabidopsis mutant displaying impaired stomatal responses to CO2 , cdi4, was isolated by a leaf thermal imaging screening. The mutated gene PECT1 encodes CTP:phosphorylethanolamine (PE) cytidylyltransferase. The cdi4 exhibited a decrease in PE levels and a defect in light-induced stomatal opening as well as low CO2 -induced stomatal opening. We created RNAi lines in which PECT1 was specifically repressed in guard cells. These lines are impaired in their stomatal responses to low CO2 concentrations or light. Fungal toxin fusicoccin (FC) promotes stomatal opening by activating plasma membrane H+ -ATPases in guard cells via phosphorylation. Arabidopsis H+ -ATPase1 (AHA1) has been reported to be highly expressed in guard cells and its activation by FC induces stomatal opening. The cdi4 and PECT1 RNAi lines displayed a reduced stomatal opening response to FC. However, similar to in the wild type, cdi4 maintained normal levels of phosphorylation and activation of the stomatal H+ -ATPases after FC treatment. Furthermore, the cdi4 displayed normal localization of GFP-AHA1 fusion protein and normal levels of AHA1 transcripts. Based on these results, we discuss how PECT1 could regulate CO2 - and light-induced stomatal movements in guard cells in a manner that is independent and downstream of the activation of H+ -ATPases.

DOI： 10.1111/tpj.16245

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Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

Abstract

Grafting is a plant propagation technique widely used in agriculture. A recent discovery of the capability of interfamily grafting in Nicotiana has expanded the potential combinations of grafting. In this study, we showed that xylem connection is essential for the achievement of interfamily grafting and investigated the molecular basis of xylem formation at the graft junction. Transcriptome and gene network analyses revealed gene modules for tracheary element (TE) formation during grafting that include genes associated with xylem cell differentiation and immune response. The reliability of the drawn network was validated by examining the role of the Nicotiana benthamiana XYLEM CYSTEINE PROTEASE (NbXCP) genes in TE formation during interfamily grafting. Promoter activities of NbXCP1 and NbXCP2 genes were found in differentiating TE cells in the stem and callus tissues at the graft junction. Analysis of a Nbxcp1;Nbxcp2 loss-of-function mutant indicated that NbXCPs control the timing of de novo TE formation at the graft junction. Moreover, grafts of the NbXCP1 overexpressor increased the scion growth rate as well as the fruit size. Thus, we identified gene modules for TE formation at the graft boundary and demonstrated potential ways to enhance Nicotiana interfamily grafting.

DOI： 10.1093/hr/uhad072

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Language：English   Publishing type：Research paper (scientific journal)  

Excess boron (B) is toxic to plants and thereby causes DNA damage and cell death in root meristems. However, the underlying mechanisms which link boron and DNA damage remain unclear. It has been reported that the rpt5a-6 mutant of the 26S proteasome is sensitive to excess boron, resulting in more frequent cell death in root meristem and reduced root elongation. In this study, we showed that a reduction in root growth in the rpt5a mutant in the presence of high boron levels is repressed by a mutation in NAC domain containing transcription factor NAC103, a substrate of the proteasome, which functions in the unfolded protein response pathway. The mutation in NAC103 alleviated excess-B-induced DNA damage and cell death in root meristems of the rpt5a mutant. Superoxide ( O 2 - ) staining with nitroblue tetrazolium revealed that boron stress causes O 2 - accumulation in root tips, which was higher in the rpt5a-6 mutant, whereas the accumulation was lower in the rpt5a-6 nac103-3 double mutant. Our work demonstrates the overall involvement of NAC103 in maintaining healthy root meristem under excess boron conditions in the absence of RPT5A proteasome subunit.

DOI： 10.3389/fpls.2023.1099816

PubMed

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Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

Iron (Fe) is an essential micronutrient for plant growth and development, participating in many significant biological processes including photosynthesis, respiration, and nitrogen fixation. Although abundant in the earth's crust, most Fe is oxidized and difficult for plants to absorb under aerobic and alkaline pH conditions. Plants, therefore, have evolved complex means to optimize their Fe-acquisition efficiency. In the past two decades, regulatory networks of transcription factors and ubiquitin ligases have proven to be essential for plant Fe uptake and translocation. Recent studies in Arabidopsis thaliana (Arabidopsis) suggest that in addition to the transcriptional network, IRON MAN/FE-UPTAKE-INDUCING PEPTIDE (IMA/FEP) peptide interacts with a ubiquitin ligase, BRUTUS (BTS)/BTS-LIKE (BTSL). Under Fe-deficient conditions, IMA/FEP peptides compete with IVc subgroup bHLH transcription factors (TFs) to interact with BTS/BTSL. The resulting complex inhibits the degradation of these TFs by BTS/BTSL, which is important for maintaining the Fe-deficiency response in roots. Furthermore, IMA/FEP peptides control systemic Fe signaling. By organ-to-organ communication in Arabidopsis, Fe deficiency in one part of a root drives the upregulation of a high-affinity Fe-uptake system in other root regions surrounded by sufficient levels of Fe. IMA/FEP peptides regulate this compensatory response through Fe-deficiency-triggered organ-to-organ communication. This mini-review summarizes recent advances in understanding how IMA/FEP peptides function in the intracellular signaling of the Fe-deficiency response and systemic Fe signaling to regulate Fe acquisition.

DOI： 10.3389/fpls.2023.1107405

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Language：Japanese   Publisher：一般社団法人 日本土壌肥料学会  

DOI： 10.20710/dohikouen.68.0_46_1

CiNii Research

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Language：English   Publishing type：Research paper (scientific journal)  

Plasmodesmata are unique channel structures in plants that link the fluid cytoplasm between adjacent cells. Plants have evolved these microchannels to allow trafficking of nutritious substances as well as regulatory factors for intercellular communication. However, tracking the behavior of plasmodesmata in real time is difficult because they are located inside tissues. Hence, a system was constructed to monitor the movement of substances by plasmodesmata using tobacco BY-2 cells, which are linearly organized cells, and a microfluidic device that traps them in place and facilitates observation. After targeting one cell for photobleaching, recovery of the lost H2B-GFP protein was detected within 200 min. No recovery was detected in that time frame by photobleaching the entire cell filaments. This suggested that the recovery of H2B-GFP protein was not due to de novo protein synthesis, but rather to translocation from neighboring cells. The transport of H2B-GFP protein was not observed when sodium chloride, a compound known to cause plasmodesmata closure, was present in the microfluid channel. Thus, using the microfluidic device and BY-2 cells, it was confirmed that the behavior of plasmodesmata could be observed in real time under controllable conditions.

DOI： 10.1007/s10265-022-01406-8

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1093/plphys/kiac025

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1371/journal.pone.0266982

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Language：Japanese   Publisher：一般社団法人 日本土壌肥料学会  

DOI： 10.20710/dohikouen.67.0_61_2

CiNii Research

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Language：Japanese   Publisher：一般社団法人 日本土壌肥料学会  

DOI： 10.20710/dohikouen.66.0_55_2

CiNii Research

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/tpj.14768

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1104/pp.19.00784

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Publisher：Cold Spring Harbor Laboratory  

Summary

Grafting is an important technique in agriculture to obtain several good traits such as high disease tolerance and high yield by exchanging root system. However, the underlined cellular processes to compensate the wound damage and repair tissues were largely unknown.

We analyzed two graft combinations: Nicotiana benthamiana (Nb) homograft as a compatible, wound repairing model and Nb heterograft with Arabidopsis thaliana (At) as an incompatible and more stressful model, which we recently identified as an exceptional maintainable interfamily grafting.

In both graft combinations, nutrient loss was observed in gene expression after grafting, where the level of nutrient loss was more sever in heterografts. Transmission electron micrographs of Nb/At heterografts suggested that microautophagy was induced in cells near the graft boundary. In At seedling micrografting, the fluorescence of autophagy protein marker GFP-ATG8 was highly observed at graft junction especially in cambial region. In At atg2 mutant homografts, growth after grafting decreased compared with wild-type homografts. Moreover, when NbATG5 knocked-down Nb scion was grafted to At stock, the successful rate of grafting was significantly decreased.

Altogether, these results suggest that component of autophagy is induced during grafting and has a role in wound healing.

DOI： 10.1101/2020.02.14.949453

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Language：Japanese   Publisher：Japanese Society of Soil Science and Plant Nutrition  

DOI： 10.20710/dojo.91.2_94

CiNii Research

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1371/journal.pgen.1007997

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3389/fpls.2016.00834

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/nph.13520

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.molp.2014.11.015

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1242/dev.113167

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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.15252/embr.201438660

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Scopus

PubMed

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Authorship：Lead author   Language：English  

DOI： 10.3389/fpls.2014.00311

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1107/S2053230X13033645

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Language：Japanese   Publisher：一般社団法人 日本土壌肥料学会  

DOI： 10.20710/dohikouen.60.0_65_2

CiNii Research

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Language：English   Publishing type：Research paper (scientific journal)  

Small polypeptides are widely used as signaling molecules in cell-to-cell communication in animals and plants. The CLAVATA3/EMBRYO SURROUNDING REGION-RELATED (CLE) gene family is composed of numerous genes that contain conserved CLE domains in various plant species and plant-parasitic nematodes. Here, we review recent progress in our understanding of CLE signaling during stem cell maintenance in Arabidopsis and grasses. We also summarize the roles of CLE signaling in the legume-Rhizobium symbiosis and infection by plant-parasitic nematodes. CLE signaling is important for diverse aspects of cell-to-cell signaling and long-distance communication, which are critical for survival, and the basic components of the CLE signaling pathway are evolutionarily conserved in both plants and animals.

DOI： 10.1016/j.pbi.2013.08.008

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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.4161/psb.22534

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Language：Japanese   Publisher：一般社団法人 日本土壌肥料学会  

DOI： 10.20710/dohikouen.58.0_72_1

CiNii Research

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Authorship：Lead author  

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.4161/psb.6.1.14128

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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1093/pcp/pcp176

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1105/tpc.107.055624

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1271/bbb.70216

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J-GLOBAL

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J-GLOBAL

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