Updated on 2026/03/07

写真a

 
YOSHIMOTO KOKI
 
Organization
Undergraduate School School of Agriculture Professor
Title
Professor
External link

Research Interests

  • 細胞死

  • 老化

  • オルガネラ

  • タンパク質分解

  • 液胞

  • オートファジー

Research Areas

  • Life sciences / Plants: molecular biology and physiology

  • Life sciences / Cell biology

  • Life sciences / Plant nutrition, soil science

Research History

  • Meiji University   School of Agriculture Department of Life Sciences   Professor

    2021.4

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  • Associate Professor

    2016.4 - 2021.3

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  • Meiji University

    2015.12 - 2016.3

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  • Researcher

    2011.12 - 2015.12

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  • Researcher   Researcher

    2010.4 - 2011.12

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  • Researcher

    2007.4 - 2010.3

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  • 大学共同利用機関法人 自然科学研究機構 基礎生物学研究所   博士研究員

    2004.4 - 2007.3

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  • Researcher

    2001.9 - 2004.3

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Professional Memberships

Committee Memberships

  • 日本植物学会   日本植物学会第88回大会(宇都宮)大会実行委員  

    2024   

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  • 植物化学調節学会   植物化学調節学会 第58回大会(川崎) 大会実行委員  

    2023   

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    Committee type:Academic society

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  • Frontiers in Plant Science   Associate Editor  

    2022.8   

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  • 日本植物学会   代議員  

    2022.6 - 2024.6   

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    Committee type:Academic society

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  • ベルギー学術研究財団フランダース(FWO)   審査委員会パネルメンバー  

    2021.1   

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    Committee type:Government

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  • 日本植物学会   Journal of Plant Research 編集委員  

    2021.1 - 2024.12   

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    Committee type:Academic society

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  • 日本植物生理学会   男女共同参画委員長  

    2020.1 - 2022.3   

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    Committee type:Academic society

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  • 日本植物生理学会   男女共同参画委員  

    2018.1 - 2019.12   

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Papers

  • Preparation of intact tissue for microscopic analysis of the endosperm cell layer in developing and mature Arabidopsis seeds Reviewed

    219   e68217   2025.5

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  • Autophagy is induced during plant grafting to promote wound healing Reviewed

    Nat. Commun.   16 ( 1 )   e3483   2025.4

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  • 種子の長期に渡る発芽能力維持に貢献するオートファジー Invited Reviewed

    篠崎大樹, 瀬田京介, 吉本光希

    化学と生物   63 ( 2 )   57 - 59   2025.2

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  • 自食作用を介した植物体内の金属資源リサイクルマシナリー

    篠崎大樹, 吉本光希

    金属   94 ( 7 )   47 - 54   2024.7

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  • Autophagy maintains endosperm quality during seed storage to preserve germination ability in Arabidopsis Reviewed

    Shinozaki, D, Takayama, E, Kawakami, N, Yoshimoto, K

    Proc. Natl. Acad. Sci. USA.   121 ( 14 )   e2321612121   2024.4

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    DOI: 10.1073/pnas.2321612121

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  • New insights into plant autophagy: molecular mechanisms and roles in development and stress responses Invited Reviewed

    Yagyu, M, Yoshimoto, K

    J. Exp. Bot.   75 ( 5 )   1234 - 1251   2024.2

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Oxford University Press (OUP)  

    Abstract

    Autophagy is an evolutionarily conserved eukaryotic intracellular degradation process. Although the molecular mechanisms of plant autophagy share similarities with those in yeast and mammals, certain unique mechanisms have been identified. Recent studies have highlighted the importance of autophagy during vegetative growth stages as well as in plant-specific developmental processes, such as seed development, germination, flowering, and somatic reprogramming. Autophagy enables plants to adapt to and manage severe environmental conditions, such as nutrient starvation, high-intensity light stress, and heat stress, leading to intracellular remodeling and physiological changes in response to stress. In the past, plant autophagy research lagged behind similar studies in yeast and mammals; however, recent advances have greatly expanded our understanding of plant-specific autophagy mechanisms and functions. This review summarizes current knowledge and latest research findings on the mechanisms and roles of plant autophagy with the objective of improving our understanding of this vital process in plants.

    DOI: 10.1093/jxb/erad459

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  • Intracellular phosphate recycling systems for survival during phosphate starvation in plants Invited Reviewed

    Yoshitake, Y, Yoshimoto, K

    Front. Plant Sci.   13   e108821   2023.1

  • Pexophagy suppresses ROS-induced damage in leaf cells under high-intensity light Reviewed International journal

    Oikawa, K, Goto-Yamada, S, Hayashi, Y, Takahashi, D, Kimori, Y, Shibata, M, Yoshimoto, K, Takemiya, A, Kondo, M, Kato, A, Shimoda, K, Ueda, H, Uemura, M, Numata, K, Ohsumi, Y, Hara-Nishimura, I, Mano, S, Yamada, K, Nishimura, M

    Nat. Commun.   13 ( 1 )   e7493   2022.12

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    Although light is essential for photosynthesis, it has the potential to elevate intracellular levels of reactive oxygen species (ROS). Since high ROS levels are cytotoxic, plants must alleviate such damage. However, the cellular mechanism underlying ROS-induced leaf damage alleviation in peroxisomes was not fully explored. Here, we show that autophagy plays a pivotal role in the selective removal of ROS-generating peroxisomes, which protects plants from oxidative damage during photosynthesis. We present evidence that autophagy-deficient mutants show light intensity-dependent leaf damage and excess aggregation of ROS-accumulating peroxisomes. The peroxisome aggregates are specifically engulfed by pre-autophagosomal structures and vacuolar membranes in both leaf cells and isolated vacuoles, but they are not degraded in mutants. ATG18a-GFP and GFP-2×FYVE, which bind to phosphatidylinositol 3-phosphate, preferentially target the peroxisomal membranes and pre-autophagosomal structures near peroxisomes in ROS-accumulating cells under high-intensity light. Our findings provide deeper insights into the plant stress response caused by light irradiation.

    DOI: 10.1038/s41467-022-35138-z

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  • Seed dormancy 4 like1 (SFL1) of Arabidopsis is a key regulator of phase transition from embryo to vegetative development Reviewed

    Zheng, L, Otani, M, Kanno, Y, Seo, M, Yoshitake, Y, Yoshimoto, K, Sugimoto, K, Kawakami, N

    Plant J.   112 ( 2 )   460 - 475   2022.10

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    DOI: 10.1111/tpj.15959

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  • Autophagy triggered by iron mediated ER stress is an important stress response to the early phase of Pi starvation in plants Reviewed

    Yoshitake, Y, Shinozaki, D, Yoshimoto, K

    Plant J.   110 ( 5 )   1370 - 1381   2022.6

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    SUMMARY

    Inorganic phosphate (Pi) is essential for plant growth. However, Pi is often limiting in soil. Hence, plants have established several mechanisms of response to Pi starvation. One of the important mechanisms is Pi recycling, which includes membrane lipid remodeling and plastid DNA degradation via catabolic enzymes. However, the involvement of other degradation systems in Pi recycling remains unclear. Autophagy, a system for degradation of intracellular components, contributes to recycling of some nutrients, such as nitrogen, carbon, and zinc, under starvation. In the present study, we found that autophagy‐deficient mutants depleted Pi early and exhibited severe leaf growth defects under Pi starvation. The main cargo of autophagy induced by early Pi depleted conditions was the endoplasmic reticulum (ER), indicating that ER‐phagy, a type of autophagy that selectively degrades the ER, is involved in the response to the early phase of Pi starvation for contribution to Pi recycling. This ER‐phagy was suppressed in an INOSITOL‐REQUIRING ENZYME 1 double mutant, ire1a ire1b, in which ER stress responses are defective, suggesting that the early Pi starvation induced ER‐phagy is induced by ER stress. Furthermore, iron limitation and inhibition of lipid‐reactive oxygen species accumulation suppressed the ER‐phagy. Interestingly, membrane lipid remodeling, a response to late Pi starvation, was accelerated in the ire1a ire1b under early Pi‐depleted conditions. Our findings reveal the existence of two different phases of responses to Pi starvation (i.e. early and late) and indicate that ER stress‐mediated ER‐phagy is involved in Pi recycling in the early phase to suppress acceleration of the late phase.

    DOI: 10.1111/tpj.15743

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    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/tpj.15743

  • The role of reticulophagy under early phase phosphate starvation in plant cells

    Yoshitake, Y, Yoshimoto, K

    Autophagy Rep.   1 ( 1 )   256 - 259   2022.6

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  • Autophagy balances the zinc–iron seesaw caused by Zn-stress Invited Reviewed

    Shinozaki, D, Yoshimoto, K

    Trends Plant Sci.   26 ( 9 )   882 - 884   2021.9

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    DOI: 10.1016/j.tplants.2021.06.014

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  • A proposed role for endomembrane trafficking processes in regulating tonoplast content and vacuole dynamics under ammonium stress conditions in Arabidopsis root cells Invited Reviewed

    Robert, G, Yagyu, M, Lascano, R, Masclaux-Daubresse, C, Yoshimoto, K

    Plant Signal. Behav.   16 ( 9 )   e192497   2021.7

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  • RCB-mediated chlorophagy caused by oversupply of nitrogen suppresses phosphate-starvation stress in plants Reviewed International journal

    Yoshitake, Y., Nakamura, S., Shinozaki, D., Izumi, M., Yoshimoto, K., Ohta, H., Shimojima, M.

    Plant Physiol.   185 ( 2 )   318 - 330   2021.3

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  • Optimal distribution of iron to sink organs via autophagy is important for tolerance to excess zinc in Arabidopsis Reviewed International journal

    Shinozaki, D, Tanoi, K, Yoshimoto, K

    Plant Cell Physiol.   62 ( 3 )   515 - 527   2021.3

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  • Ammonium stress increases microautophagic activity while impairing macroautophagic flux in Arabidopsis roots Reviewed International journal

    Robert, G, Yagyu, M, Koizumi, T, Naya, L, Masclaux-Daubresse, C, Yoshimoto, K

    Plant J.   105 ( 4 )   1083 - 1097   2021.2

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  • Thaumatin-like proteins and a cysteine protease inhibitor secreted by the pine wood nematode <I>Bursaphelenchus xylophilus</I> induce cell death in <I>Nicotiana benthamiana</I> Reviewed International journal

    Kirino, H., Yoshimoto, K., Shinya, R.

    PLOS ONE   15 ( 10 )   e0241613   2020.10

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    DOI: 10.1371/journal.pone.0241613

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  • Editorial: Organelle autophagy in plant development Invited Reviewed International journal

    Izumi, M., Yoshimoto, K., and Batoko, H.

    Front. Plant Sci.   11   e502   2020.5

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  • Importance of non-systemic leaf autophagy for suppression of zinc starvation induced-chlorosis Invited Reviewed International journal

    Shinozaki, D, Notaguchi, M, Yoshimoto, K

    Plant Signal. Behav.   15 ( 5 )   e174604   2020.5

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Taylor & Francis  

    Shinozaki, D., Notaguchi, M., and <U>Yoshimoto, K.</U>

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  • Autophagy increases zinc bioavailability to avoid light-mediated reactive oxygen species production under zinc deficiency Reviewed International journal

    Shinozaki, D, Merkulova, A. E, Naya, L, Horie, T, Kanno, Y, Seo, M, Ohsumi, Y, Masclaux-Daubresse, C, Yoshimoto, K

    Plant Physiol.   182 ( 3 )   1284 - 1296   2020.3

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    Zinc (Zn) is an essential micronutrient for plant growth. Accordingly, Zn deficiency (-Zn) in agricultural fields is a serious problem, especially in developing regions. Autophagy, a major intracellular degradation system in eukaryotes, plays important roles in nutrient recycling under nitrogen and carbon starvation. However, the relationship between autophagy and deficiencies of other essential elements remains poorly understood, especially in plants. In this study, we focused on Zn due to the property that within cells most Zn is tightly bound to proteins, which can be targets of autophagy. We found that autophagy plays a critical role during -Zn in Arabidopsis (Arabidopsis thaliana). Autophagy-defective plants (atg mutants) failed to grow and developed accelerated chlorosis under -Zn. As expected, -Zn induced autophagy in wild-type plants, whereas in atg mutants, various organelle proteins accumulated to high levels. Additionally, the amount of free Zn2+ was lower in atg mutants than in control plants. Interestingly, -Zn symptoms in atg mutants recovered under low-light, iron-limited conditions. The levels of hydroxyl radicals in chloroplasts were elevated, and the levels of superoxide were reduced in -Zn atg mutants. These results imply that the photosynthesis-mediated Fenton-like reaction, which is responsible for the chlorotic symptom of -Zn, is accelerated in atg mutants. Together, our data indicate that autophagic degradation plays important functions in maintaining Zn pools to increase Zn bioavailability and maintain reactive oxygen species homeostasis under -Zn in plants.

    DOI: 10.1104/pp.19.01522

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  • Autophagy and nutrients management in plants Invited Reviewed

    Chen, Q, Shinozaki, D, Luo, J, Pottier, M, Havé, M, Marmagne, A, Reisdorf-Cren, M, Chardon, F, Thomine, S, Yoshimoto, K, Masclaux-Daubresse, C

    Cells   8 ( 11 )   e1426   2019.11

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    Nutrient recycling and mobilization from organ to organ all along the plant lifespan is essential for plant survival under changing environments. Nutrient remobilization to the seeds is also essential for good seed production. In this review, we summarize the recent advances made to understand how plants manage nutrient remobilization from senescing organs to sink tissues and what is the contribution of autophagy in this process. Plant engineering manipulating autophagy for better yield and plant tolerance to stresses will be presented.

    DOI: 10.3390/cells8111426

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  • 植物の必須栄養素から考える植物オートファジーの重要性 Invited Reviewed

    吉本光希

    生化学   91 ( 5 )   652 - 658   2019.10

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  • 植物の環境適応におけるオートファジーの重要性 Invited

    吉本光希

    アミノ酸研究   12 ( 2 )   89 - 96   2018.12

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:日本アミノ酸学会  

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  • Unveiling the molecular mechanisms of plant autophagy - From autophagosomes to vacuoles in plants Invited Reviewed International journal

    Yoshimoto, K, Ohsumi, Y

    Plant Cell Physiol.   59 ( 7 )   1337 - 1344   2018.7

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  • Autophagy controls resource allocations and protein storage accumulation in Arabidopsis seeds Reviewed International journal

    Di Berardino, J., Marmagne, A., Berger, A., Yoshimoto, K., Cueff, G., Chardon, F., Masclaux-Daubresse, C., Reisdorf-Cren, M.

    J. Exp. Bot.   69 ( 6 )   1403 - 1414   2018.3

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  • Physiological meanings of autophagy in plants ~aiming to elucidate plant specific functions of autophagy~ Invited Reviewed

    BSJ-Review   9 ( A )   3 - 10   2018

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.24480/bsj-review.9a2.00128

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  • 植物におけるオートファジーの意義と役割 Invited Reviewed

    吉本光希

    化学と生物   52 ( 8 )   535 - 540   2014.8

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:日本農芸化学会  

    DOI: 10.1271/kagakutoseibutsu.52.535

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  • Autophagy, plant senescence, and nutrient recycling Invited Reviewed

    Avila-Ospina, L, Moison, M, Yoshimoto, K, Masclaux-Daubresse, C

    J. Exp. Bot.   65 ( 14 )   3799 - 3811   2014.7

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    DOI: 10.1093/jxb/eru039

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  • Plant autophagy is responsible for peroxisomal transition and plays an important role in the maintenance of peroxisomal quality Invited Reviewed

    Shibata, M, Oikawa, K, Yoshimoto, K, Goto-Yamada, S, Mano, S, Yamada, K, Kondo, M, Hayashi, M, Sakamoto, W, Ohsumi, Y, Nishimura, M

    Autophagy   10 ( 5 )   936 - 937   2014.5

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    DOI: 10.4161/auto.28529

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  • Stitching together the multiple dimensions of autophagy using metabolomics and transcriptomics reveals impacts on metabolism, development, and plant responses to the environment in Arabidopsis Reviewed

    Masclaux-Daubresse, C, Clement, G, Anne, P, Routaboul, JM, Guiboileau, A, Soulay, F, Shirasu, K, Yoshimoto, K

    Plant Cell   26 ( 5 )   1857 - 1877   2014.5

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    DOI: 10.1105/tpc.114.124677

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  • Assessment and optimization of autophagy monitoring methods in Arabidopsis roots indicate direct fusion of autophagosomes with vacuoles Invited Reviewed

    Merkulova, EA, Guiboileau, A, Naya, L, Masclaux-Daubresse, C, Yoshimoto, K

    Plant Cell Physiol.   55 ( 4 )   715 - 726   2014.4

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    DOI: 10.1093/pcp/pcu041

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  • Organ-specific quality control of plant peroxisomes is mediated by autophagy Reviewed

    Yoshimoto, K, Shibata, M, Kondo, M, Oikawa, K, Sato, M, Toyooka, K, Shirasu, K, Nishimura, M, Ohsumi, Y

    J. Cell Sci.   127 ( 6 )   1161 - 1168   2014.3

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    DOI: 10.1242/jcs.139709

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  • Highly oxidized peroxisomes are selectively degraded via autophagy in Arabidopsis Reviewed

    Shibata, M, Oikawa, K, Yoshimoto, K, Kondo, M, Mano, S, Yamada, K, Hayashi, M, Sakamoto, W, Ohsumi, Y, Nishimura, M

    Plant Cell   26 ( 3 )   1377 - 1377   2014.3

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  • Autophagy as a possible mechanism for micronutrient remobilization from leaves to seeds Reviewed

    Pottier, M, Masclaux-Daubresse, C, Yoshimoto, K, Thomine, S

    Front. Plant Sci.   5   2014.1

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    DOI: 10.3389/fpls.2014.00011

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  • Highly oxidized peroxisomes are selectively degraded via autophagy in Arabidopsis Reviewed

    Shibata, M, Oikawa, K, Yoshimoto, K, Kondo, M, Mano, S, Yamada, K, Hayashi, M, Sakamoto, W, Ohsumi, Y, Nishimura, M

    PLANT CELL   25 ( 12 )   4967 - 4983   2013.12

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    DOI: 10.1105/tpc.113.116947

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  • Physiological and metabolic consequences of autophagy deficiency for the management of nitrogen and protein resources in Arabidopsis leaves depending on nitrate availability Reviewed

    Guiboileau, A, Avila-Ospina, L, Yoshimoto, K, Soulay, F, Azzopardi, M, Marmagne, A, Lothier, J, Masclaux-Daubresse, C

    New Phytol.   199 ( 3 )   683 - 694   2013.8

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    DOI: 10.1111/nph.12307

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  • A possible involvement of autophagy in amyloplast degradation in columella cells during hydrotropic response of Arabidopsis roots Reviewed

    Nakayama, M, Kaneko, Y, Miyazawa, Y, Fujii, N, Higashitani, N, Wada, S, Ishida, H, Yoshimoto, K, hirasu, K, Yamada, K, Nishimura, M, Takahashi, H

    Planta   236 ( 4 )   999 - 1012   2012.10

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    DOI: 10.1007/s00425-012-1655-5

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  • Beginning to understand autophagy, an intracellularself-degradation system in plants Invited Reviewed

    Yoshimoto, K

    Plant Cell Physiol.   53 ( 8 )   1355 - 1365   2012.8

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    DOI: 10.1093/pcp/pcs099

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  • Autophagy machinery controls nitrogen remobilization at the whole-plant level under both limiting and ample nitrate conditions in Arabidopsis Reviewed

    Guiboileau, A, Yoshimoto, K, Soulay, F, Bataille, MP, Avice, JC, Masclaux-Daubresse, C

    New Phytol.   194 ( 3 )   732 - 740   2012.5

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    DOI: 10.1111/j.1469-8137.2012.04084.x

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  • Guidelines for the use and interpretation of assays for monitoring autophagy. Invited Reviewed International journal

    Daniel J Klionsky, Fabio C Abdalla, Hagai Abeliovich, Robert T Abraham, Abraham Acevedo-Arozena, Khosrow Adeli, Lotta Agholme, Maria Agnello, Patrizia Agostinis, Julio A Aguirre-Ghiso, Hyung Jun Ahn, Ouardia Ait-Mohamed, Slimane Ait-Si-Ali, Takahiko Akematsu, Shizuo Akira, Hesham M Al-Younes, Munir A Al-Zeer, Matthew L Albert, Roger L Albin, Javier Alegre-Abarrategui, Maria Francesca Aleo, Mehrdad Alirezaei, Alexandru Almasan, Maylin Almonte-Becerril, Atsuo Amano, Ravi Amaravadi, Shoba Amarnath, Amal O Amer, Nathalie Andrieu-Abadie, Vellareddy Anantharam, David K Ann, Shailendra Anoopkumar-Dukie, Hiroshi Aoki, Nadezda Apostolova, Giuseppe Arancia, John P Aris, Katsuhiko Asanuma, Nana Y O Asare, Hisashi Ashida, Valerie Askanas, David S Askew, Patrick Auberger, Misuzu Baba, Steven K Backues, Eric H Baehrecke, Ben A Bahr, Xue-Yuan Bai, Yannick Bailly, Robert Baiocchi, Giulia Baldini, Walter Balduini, Andrea Ballabio, Bruce A Bamber, Edward T W Bampton, Gábor Bánhegyi, Clinton R Bartholomew, Diane C Bassham, Robert C Bast Jr, Henri Batoko, Boon-Huat Bay, Isabelle Beau, Daniel M Béchet, Thomas J Begley, Christian Behl, Christian Behrends, Soumeya Bekri, Bryan Bellaire, Linda J Bendall, Luca Benetti, Laura Berliocchi, Henri Bernardi, Francesca Bernassola, Sébastien Besteiro, Ingrid Bhatia-Kissova, Xiaoning Bi, Martine Biard-Piechaczyk, Janice S Blum, Lawrence H Boise, Paolo Bonaldo, David L Boone, Beat C Bornhauser, Karina R Bortoluci, Ioannis Bossis, Frédéric Bost, Jean-Pierre Bourquin, Patricia Boya, Michaël Boyer-Guittaut, Peter V Bozhkov, Nathan R Brady, Claudio Brancolini, Andreas Brech, Jay E Brenman, Ana Brennand, Emery H Bresnick, Patrick Brest, Dave Bridges, Molly L Bristol, Paul S Brookes, Eric J Brown, John H Brumell, Nicola Brunetti-Pierri, Ulf T Brunk, Dennis E Bulman, Scott J Bultman, Geert Bultynck, Lena F Burbulla, Wilfried Bursch, Jonathan P Butchar, Wanda Buzgariu, Sergio P Bydlowski, Ken Cadwell, Monika Cahová, Dongsheng Cai, Jiyang Cai, Qian Cai, Bruno Calabretta, Javier Calvo-Garrido, Nadine Camougrand, Michelangelo Campanella, Jenny Campos-Salinas, Eleonora Candi, Lizhi Cao, Allan B Caplan, Simon R Carding, Sandra M Cardoso, Jennifer S Carew, Cathleen R Carlin, Virginie Carmignac, Leticia A M Carneiro, Serena Carra, Rosario A Caruso, Giorgio Casari, Caty Casas, Roberta Castino, Eduardo Cebollero, Francesco Cecconi, Jean Celli, Hassan Chaachouay, Han-Jung Chae, Chee-Yin Chai, David C Chan, Edmond Y Chan, Raymond Chuen-Chung Chang, Chi-Ming Che, Ching-Chow Chen, Guang-Chao Chen, Guo-Qiang Chen, Min Chen, Quan Chen, Steve S-L Chen, WenLi Chen, Xi Chen, Xiangmei Chen, Xiequn Chen, Ye-Guang Chen, Yingyu Chen, Yongqiang Chen, Yu-Jen Chen, Zhixiang Chen, Alan Cheng, Christopher H K Cheng, Yan Cheng, Heesun Cheong, Jae-Ho Cheong, Sara Cherry, Russ Chess-Williams, Zelda H Cheung, Eric Chevet, Hui-Ling Chiang, Roberto Chiarelli, Tomoki Chiba, Lih-Shen Chin, Shih-Hwa Chiou, Francis V Chisari, Chi Hin Cho, Dong-Hyung Cho, Augustine M K Choi, DooSeok Choi, Kyeong Sook Choi, Mary E Choi, Salem Chouaib, Divaker Choubey, Vinay Choubey, Charleen T Chu, Tsung-Hsien Chuang, Sheau-Huei Chueh, Taehoon Chun, Yong-Joon Chwae, Mee-Len Chye, Roberto Ciarcia, Maria R Ciriolo, Michael J Clague, Robert S B Clark, Peter G H Clarke, Robert Clarke, Patrice Codogno, Hilary A Coller, María I Colombo, Sergio Comincini, Maria Condello, Fabrizio Condorelli, Mark R Cookson, Graham H Coombs, Isabelle Coppens, Ramon Corbalan, Pascale Cossart, Paola Costelli, Safia Costes, Ana Coto-Montes, Eduardo Couve, Fraser P Coxon, James M Cregg, José L Crespo, Marianne J Cronjé, Ana Maria Cuervo, Joseph J Cullen, Mark J Czaja, Marcello D'Amelio, Arlette Darfeuille-Michaud, Lester M Davids, Faith E Davies, Massimo De Felici, John F de Groot, Cornelis A M de Haan, Luisa De Martino, Angelo De Milito, Vincenzo De Tata, Jayanta Debnath, Alexei Degterev, Benjamin Dehay, Lea M D Delbridge, Francesca Demarchi, Yi Zhen Deng, Jörn Dengjel, Paul Dent, Donna Denton, Vojo Deretic, Shyamal D Desai, Rodney J Devenish, Mario Di Gioacchino, Gilbert Di Paolo, Chiara Di Pietro, Guillermo Díaz-Araya, Inés Díaz-Laviada, Maria T Diaz-Meco, Javier Diaz-Nido, Ivan Dikic, Savithramma P Dinesh-Kumar, Wen-Xing Ding, Clark W Distelhorst, Abhinav Diwan, Mojgan Djavaheri-Mergny, Svetlana Dokudovskaya, Zheng Dong, Frank C Dorsey, Victor Dosenko, James J Dowling, Stephen Doxsey, Marlène Dreux, Mark E Drew, Qiuhong Duan, Michel A Duchosal, Karen Duff, Isabelle Dugail, Madeleine Durbeej, Michael Duszenko, Charles L Edelstein, Aimee L Edinger, Gustavo Egea, Ludwig Eichinger, N Tony Eissa, Suhendan Ekmekcioglu, Wafik S El-Deiry, Zvulun Elazar, Mohamed Elgendy, Lisa M Ellerby, Kai Er Eng, Anna-Mart Engelbrecht, Simone Engelender, Jekaterina Erenpreisa, Ricardo Escalante, Audrey Esclatine, Eeva-Liisa Eskelinen, Lucile Espert, Virginia Espina, Huizhou Fan, Jia Fan, Qi-Wen Fan, Zhen Fan, Shengyun Fang, Yongqi Fang, Manolis Fanto, Alessandro Fanzani, Thomas Farkas, Jean-Claude Farré, Mathias Faure, Marcus Fechheimer, Carl G Feng, Jian Feng, Qili Feng, Youji Feng, László Fésüs, Ralph Feuer, Maria E Figueiredo-Pereira, Gian Maria Fimia, Diane C Fingar, Steven Finkbeiner, Toren Finkel, Kim D Finley, Filomena Fiorito, Edward A Fisher, Paul B Fisher, Marc Flajolet, Maria L Florez-McClure, Salvatore Florio, Edward A Fon, Francesco Fornai, Franco Fortunato, Rati Fotedar, Daniel H Fowler, Howard S Fox, Rodrigo Franco, Lisa B Frankel, Marc Fransen, José M Fuentes, Juan Fueyo, Jun Fujii, Kozo Fujisaki, Eriko Fujita, Mitsunori Fukuda, Ruth H Furukawa, Matthias Gaestel, Philippe Gailly, Malgorzata Gajewska, Brigitte Galliot, Vincent Galy, Subramaniam Ganesh, Barry Ganetzky, Ian G Ganley, Fen-Biao Gao, George F Gao, Jinming Gao, Lorena Garcia, Guillermo Garcia-Manero, Mikel Garcia-Marcos, Marjan Garmyn, Andrei L Gartel, Evelina Gatti, Mathias Gautel, Thomas R Gawriluk, Matthew E Gegg, Jiefei Geng, Marc Germain, Jason E Gestwicki, David A Gewirtz, Saeid Ghavami, Pradipta Ghosh, Anna M Giammarioli, Alexandra N Giatromanolaki, Spencer B Gibson, Robert W Gilkerson, Michael L Ginger, Henry N Ginsberg, Jakub Golab, Michael S Goligorsky, Pierre Golstein, Candelaria Gomez-Manzano, Ebru Goncu, Céline Gongora, Claudio D Gonzalez, Ramon Gonzalez, Cristina González-Estévez, Rosa Ana González-Polo, Elena Gonzalez-Rey, Nikolai V Gorbunov, Sharon Gorski, Sandro Goruppi, Roberta A Gottlieb, Devrim Gozuacik, Giovanna Elvira Granato, Gary D Grant, Kim N Green, Aleš Gregorc, Frédéric Gros, Charles Grose, Thomas W Grunt, Philippe Gual, Jun-Lin Guan, Kun-Liang Guan, Sylvie M Guichard, Anna S Gukovskaya, Ilya Gukovsky, Jan Gunst, Asa B Gustafsson, Andrew J Halayko, Amber N Hale, Sandra K Halonen, Maho Hamasaki, Feng Han, Ting Han, Michael K Hancock, Malene Hansen, Hisashi Harada, Masaru Harada, Stefan E Hardt, J Wade Harper, Adrian L Harris, James Harris, Steven D Harris, Makoto Hashimoto, Jeffrey A Haspel, Shin-ichiro Hayashi, Lori A Hazelhurst, Congcong He, You-Wen He, Marie-Joseé Hébert, Kim A Heidenreich, Miep H Helfrich, Gudmundur V Helgason, Elizabeth P Henske, Brian Herman, Paul K Herman, Claudio Hetz, Sabine Hilfiker, Joseph A Hill, Lynne J Hocking, Paul Hofman, Thomas G Hofmann, Jörg Höhfeld, Tessa L Holyoake, Ming-Huang Hong, David A Hood, Gökhan S Hotamisligil, Ewout J Houwerzijl, Maria Høyer-Hansen, Bingren Hu, Chien-An A Hu, Hong-Ming Hu, Ya Hua, Canhua Huang, Ju Huang, Shengbing Huang, Wei-Pang Huang, Tobias B Huber, Won-Ki Huh, Tai-Ho Hung, Ted R Hupp, Gang Min Hur, James B Hurley, Sabah N A Hussain, Patrick J Hussey, Jung Jin Hwang, Seungmin Hwang, Atsuhiro Ichihara, Shirin Ilkhanizadeh, Ken Inoki, Takeshi Into, Valentina Iovane, Juan L Iovanna, Nancy Y Ip, Yoshitaka Isaka, Hiroyuki Ishida, Ciro Isidoro, Ken-ichi Isobe, Akiko Iwasaki, Marta Izquierdo, Yotaro Izumi, Panu M Jaakkola, Marja Jäättelä, George R Jackson, William T Jackson, Bassam Janji, Marina Jendrach, Ju-Hong Jeon, Eui-Bae Jeung, Hong Jiang, Hongchi Jiang, Jean X Jiang, Ming Jiang, Qing Jiang, Xuejun Jiang, Xuejun Jiang, Alberto Jiménez, Meiyan Jin, Shengkan Jin, Cheol O Joe, Terje Johansen, Daniel E Johnson, Gail V W Johnson, Nicola L Jones, Bertrand Joseph, Suresh K Joseph, Annie M Joubert, Gábor Juhász, Lucienne Juillerat-Jeanneret, Chang Hwa Jung, Yong-Keun Jung, Kai Kaarniranta, Allen Kaasik, Tomohiro Kabuta, Motoni Kadowaki, Katarina Kagedal, Yoshiaki Kamada, Vitaliy O Kaminskyy, Harm H Kampinga, Hiromitsu Kanamori, Chanhee Kang, Khong Bee Kang, Kwang Il Kang, Rui Kang, Yoon-A Kang, Tomotake Kanki, Thirumala-Devi Kanneganti, Haruo Kanno, Anumantha G Kanthasamy, Arthi Kanthasamy, Vassiliki Karantza, Gur P Kaushal, Susmita Kaushik, Yoshinori Kawazoe, Po-Yuan Ke, John H Kehrl, Ameeta Kelekar, Claus Kerkhoff, David H Kessel, Hany Khalil, Jan A K W Kiel, Amy A Kiger, Akio Kihara, Deok Ryong Kim, Do-Hyung Kim, Dong-Hou Kim, Eun-Kyoung Kim, Hyung-Ryong Kim, Jae-Sung Kim, Jeong Hun Kim, Jin Cheon Kim, John K Kim, Peter K Kim, Seong Who Kim, Yong-Sun Kim, Yonghyun Kim, Adi Kimchi, Alec C Kimmelman, Jason S King, Timothy J Kinsella, Vladimir Kirkin, Lorrie A Kirshenbaum, Katsuhiko Kitamoto, Kaio Kitazato, Ludger Klein, Walter T Klimecki, Jochen Klucken, Erwin Knecht, Ben C B Ko, Jan C Koch, Hiroshi Koga, Jae-Young Koh, Young Ho Koh, Masato Koike, Masaaki Komatsu, Eiki Kominami, Hee Jeong Kong, Wei-Jia Kong, Viktor I Korolchuk, Yaichiro Kotake, Michael I Koukourakis, Juan B Kouri Flores, Attila L Kovács, Claudine Kraft, Dimitri Krainc, Helmut Krämer, Carole Kretz-Remy, Anna M Krichevsky, Guido Kroemer, Rejko Krüger, Oleg Krut, Nicholas T Ktistakis, Chia-Yi Kuan, Roza Kucharczyk, Ashok Kumar, Raj Kumar, Sharad Kumar, Mondira Kundu, Hsing-Jien Kung, Tino Kurz, Ho Jeong Kwon, Albert R La Spada, Frank Lafont, Trond Lamark, Jacques Landry, Jon D Lane, Pierre Lapaquette, Jocelyn F Laporte, Lajos László, Sergio Lavandero, Josée N Lavoie, Robert Layfield, Pedro A Lazo, Weidong Le, Laurent Le Cam, Daniel J Ledbetter, Alvin J X Lee, Byung-Wan Lee, Gyun Min Lee, Jongdae Lee, Ju-Hyun Lee, Michael Lee, Myung-Shik Lee, Sug Hyung Lee, Christiaan Leeuwenburgh, Patrick Legembre, Renaud Legouis, Michael Lehmann, Huan-Yao Lei, Qun-Ying Lei, David A Leib, José Leiro, John J Lemasters, Antoinette Lemoine, Maciej S Lesniak, Dina Lev, Victor V Levenson, Beth Levine, Efrat Levy, Faqiang Li, Jun-Lin Li, Lian Li, Sheng Li, Weijie Li, Xue-Jun Li, Yan-bo Li, Yi-Ping Li, Chengyu Liang, Qiangrong Liang, Yung-Feng Liao, Pawel P Liberski, Andrew Lieberman, Hyunjung J Lim, Kah-Leong Lim, Kyu Lim, Chiou-Feng Lin, Fu-Cheng Lin, Jian Lin, Jiandie D Lin, Kui Lin, Wan-Wan Lin, Weei-Chin Lin, Yi-Ling Lin, Rafael Linden, Paul Lingor, Jennifer Lippincott-Schwartz, Michael P Lisanti, Paloma B Liton, Bo Liu, Chun-Feng Liu, Kaiyu Liu, Leyuan Liu, Qiong A Liu, Wei Liu, Young-Chau Liu, Yule Liu, Richard A Lockshin, Chun-Nam Lok, Sagar Lonial, Benjamin Loos, Gabriel Lopez-Berestein, Carlos López-Otín, Laura Lossi, Michael T Lotze, Peter Lőw, Binfeng Lu, Bingwei Lu, Bo Lu, Zhen Lu, Frédéric Luciano, Nicholas W Lukacs, Anders H Lund, Melinda A Lynch-Day, Yong Ma, Fernando Macian, Jeff P MacKeigan, Kay F Macleod, Frank Madeo, Luigi Maiuri, Maria Chiara Maiuri, Davide Malagoli, May Christine V Malicdan, Walter Malorni, Na Man, Eva-Maria Mandelkow, Stéphen Manon, Irena Manov, Kai Mao, Xiang Mao, Zixu Mao, Philippe Marambaud, Daniela Marazziti, Yves L Marcel, Katie Marchbank, Piero Marchetti, Stefan J Marciniak, Mateus Marcondes, Mohsen Mardi, Gabriella Marfe, Guillermo Mariño, Maria Markaki, Mark R Marten, Seamus J Martin, Camille Martinand-Mari, Wim Martinet, Marta Martinez-Vicente, Matilde Masini, Paola Matarrese, Saburo Matsuo, Raffaele Matteoni, Andreas Mayer, Nathalie M Mazure, David J McConkey, Melanie J McConnell, Catherine McDermott, Christine McDonald, Gerald M McInerney, Sharon L McKenna, BethAnn McLaughlin, Pamela J McLean, Christopher R McMaster, G Angus McQuibban, Alfred J Meijer, Miriam H Meisler, Alicia Meléndez, Thomas J Melia, Gerry Melino, Maria A Mena, Javier A Menendez, Rubem F S Menna-Barreto, Manoj B Menon, Fiona M Menzies, Carol A Mercer, Adalberto Merighi, Diane E Merry, Stefania Meschini, Christian G Meyer, Thomas F Meyer, Chao-Yu Miao, Jun-Ying Miao, Paul A M Michels, Carine Michiels, Dalibor Mijaljica, Ana Milojkovic, Saverio Minucci, Clelia Miracco, Cindy K Miranti, Ioannis Mitroulis, Keisuke Miyazawa, Noboru Mizushima, Baharia Mograbi, Simin Mohseni, Xavier Molero, Bertrand Mollereau, Faustino Mollinedo, Takashi Momoi, Iryna Monastyrska, Martha M Monick, Mervyn J Monteiro, Michael N Moore, Rodrigo Mora, Kevin Moreau, Paula I Moreira, Yuji Moriyasu, Jorge Moscat, Serge Mostowy, Jeremy C Mottram, Tomasz Motyl, Charbel E-H Moussa, Sylke Müller, Sylviane Muller, Karl Münger, Christian Münz, Leon O Murphy, Maureen E Murphy, Antonio Musarò, Indira Mysorekar, Eiichiro Nagata, Kazuhiro Nagata, Aimable Nahimana, Usha Nair, Toshiyuki Nakagawa, Kiichi Nakahira, Hiroyasu Nakano, Hitoshi Nakatogawa, Meera Nanjundan, Naweed I Naqvi, Derek P Narendra, Masashi Narita, Miguel Navarro, Steffan T Nawrocki, Taras Y Nazarko, Andriy Nemchenko, Mihai G Netea, Thomas P Neufeld, Paul A Ney, Ioannis P Nezis, Huu Phuc Nguyen, Daotai Nie, Ichizo Nishino, Corey Nislow, Ralph A Nixon, Takeshi Noda, Angelika A Noegel, Anna Nogalska, Satoru Noguchi, Lucia Notterpek, Ivana Novak, Tomoyoshi Nozaki, Nobuyuki Nukina, Thorsten Nürnberger, Beat Nyfeler, Keisuke Obara, Terry D Oberley, Salvatore Oddo, Michinaga Ogawa, Toya Ohashi, Koji Okamoto, Nancy L Oleinick, F Javier Oliver, Laura J Olsen, Stefan Olsson, Onya Opota, Timothy F Osborne, Gary K Ostrander, Kinya Otsu, Jing-hsiung James Ou, Mireille Ouimet, Michael Overholtzer, Bulent Ozpolat, Paolo Paganetti, Ugo Pagnini, Nicolas Pallet, Glen E Palmer, Camilla Palumbo, Tianhong Pan, Theocharis Panaretakis, Udai Bhan Pandey, Zuzana Papackova, Issidora Papassideri, Irmgard Paris, Junsoo Park, Ohkmae K Park, Jan B Parys, Katherine R Parzych, Susann Patschan, Cam Patterson, Sophie Pattingre, John M Pawelek, Jianxin Peng, David H Perlmutter, Ida Perrotta, George Perry, Shazib Pervaiz, Matthias Peter, Godefridus J Peters, Morten Petersen, Goran Petrovski, James M Phang, Mauro Piacentini, Philippe Pierre, Valérie Pierrefite-Carle, Gérard Pierron, Ronit Pinkas-Kramarski, Antonio Piras, Natik Piri, Leonidas C Platanias, Stefanie Pöggeler, Marc Poirot, Angelo Poletti, Christian Poüs, Mercedes Pozuelo-Rubio, Mette Prætorius-Ibba, Anil Prasad, Mark Prescott, Muriel Priault, Nathalie Produit-Zengaffinen, Ann Progulske-Fox, Tassula Proikas-Cezanne, Serge Przedborski, Karin Przyklenk, Rosa Puertollano, Julien Puyal, Shu-Bing Qian, Liang Qin, Zheng-Hong Qin, Susan E Quaggin, Nina Raben, Hannah Rabinowich, Simon W Rabkin, Irfan Rahman, Abdelhaq Rami, Georg Ramm, Glenn Randall, Felix Randow, V Ashutosh Rao, Jeffrey C Rathmell, Brinda Ravikumar, Swapan K Ray, Bruce H Reed, John C Reed, Fulvio Reggiori, Anne Régnier-Vigouroux, Andreas S Reichert, John J Reiners Jr, Russel J Reiter, Jun Ren, José L Revuelta, Christopher J Rhodes, Konstantinos Ritis, Elizete Rizzo, Jeffrey Robbins, Michel Roberge, Hernan Roca, Maria C Roccheri, Stephane Rocchi, H Peter Rodemann, Santiago Rodríguez de Córdoba, Bärbel Rohrer, Igor B Roninson, Kirill Rosen, Magdalena M Rost-Roszkowska, Mustapha Rouis, Kasper M A Rouschop, Francesca Rovetta, Brian P Rubin, David C Rubinsztein, Klaus Ruckdeschel, Edmund B Rucker 3rd, Assaf Rudich, Emil Rudolf, Nelson Ruiz-Opazo, Rossella Russo, Tor Erik Rusten, Kevin M Ryan, Stefan W Ryter, David M Sabatini, Junichi Sadoshima, Tapas Saha, Tatsuya Saitoh, Hiroshi Sakagami, Yasuyoshi Sakai, Ghasem Hoseini Salekdeh, Paolo Salomoni, Paul M Salvaterra, Guy Salvesen, Rosa Salvioli, Anthony M J Sanchez, José A Sánchez-Alcázar, Ricardo Sánchez-Prieto, Marco Sandri, Uma Sankar, Poonam Sansanwal, Laura Santambrogio, Shweta Saran, Sovan Sarkar, Minnie Sarwal, Chihiro Sasakawa, Ausra Sasnauskiene, Miklós Sass, Ken Sato, Miyuki Sato, Anthony H V Schapira, Michael Scharl, Hermann M Schätzl, Wiep Scheper, Stefano Schiaffino, Claudio Schneider, Marion E Schneider, Regine Schneider-Stock, Patricia V Schoenlein, Daniel F Schorderet, Christoph Schüller, Gary K Schwartz, Luca Scorrano, Linda Sealy, Per O Seglen, Juan Segura-Aguilar, Iban Seiliez, Oleksandr Seleverstov, Christian Sell, Jong Bok Seo, Duska Separovic, Vijayasaradhi Setaluri, Takao Setoguchi, Carmine Settembre, John J Shacka, Mala Shanmugam, Irving M Shapiro, Eitan Shaulian, Reuben J Shaw, James H Shelhamer, Han-Ming Shen, Wei-Chiang Shen, Zu-Hang Sheng, Yang Shi, Kenichi Shibuya, Yoshihiro Shidoji, Jeng-Jer Shieh, Chwen-Ming Shih, Yohta Shimada, Shigeomi Shimizu, Takahiro Shintani, Orian S Shirihai, Gordon C Shore, Andriy A Sibirny, Stan B Sidhu, Beata Sikorska, Elaine C M Silva-Zacarin, Alison Simmons, Anna Katharina Simon, Hans-Uwe Simon, Cristiano Simone, Anne Simonsen, David A Sinclair, Rajat Singh, Debasish Sinha, Frank A Sinicrope, Agnieszka Sirko, Parco M Siu, Efthimios Sivridis, Vojtech Skop, Vladimir P Skulachev, Ruth S Slack, Soraya S Smaili, Duncan R Smith, Maria S Soengas, Thierry Soldati, Xueqin Song, Anil K Sood, Tuck Wah Soong, Federica Sotgia, Stephen A Spector, Claudia D Spies, Wolfdieter Springer, Srinivasa M Srinivasula, Leonidas Stefanis, Joan S Steffan, Ruediger Stendel, Harald Stenmark, Anastasis Stephanou, Stephan T Stern, Cinthya Sternberg, Björn Stork, Peter Strålfors, Carlos S Subauste, Xinbing Sui, David Sulzer, Jiaren Sun, Shi-Yong Sun, Zhi-Jun Sun, Joseph J Y Sung, Kuninori Suzuki, Toshihiko Suzuki, Michele S Swanson, Charles Swanton, Sean T Sweeney, Lai-King Sy, Gyorgy Szabadkai, Ira Tabas, Heinrich Taegtmeyer, Marco Tafani, Krisztina Takács-Vellai, Yoshitaka Takano, Kaoru Takegawa, Genzou Takemura, Fumihiko Takeshita, Nicholas J Talbot, Kevin S W Tan, Keiji Tanaka, Kozo Tanaka, Daolin Tang, Dingzhong Tang, Isei Tanida, Bakhos A Tannous, Nektarios Tavernarakis, Graham S Taylor, Gregory A Taylor, J Paul Taylor, Lance S Terada, Alexei Terman, Gianluca Tettamanti, Karin Thevissen, Craig B Thompson, Andrew Thorburn, Michael Thumm, FengFeng Tian, Yuan Tian, Glauco Tocchini-Valentini, Aviva M Tolkovsky, Yasuhiko Tomino, Lars Tönges, Sharon A Tooze, Cathy Tournier, John Tower, Roberto Towns, Vladimir Trajkovic, Leonardo H Travassos, Ting-Fen Tsai, Mario P Tschan, Takeshi Tsubata, Allan Tsung, Boris Turk, Lorianne S Turner, Suresh C Tyagi, Yasuo Uchiyama, Takashi Ueno, Midori Umekawa, Rika Umemiya-Shirafuji, Vivek K Unni, Maria I Vaccaro, Enza Maria Valente, Greet Van den Berghe, Ida J van der Klei, Wouter van Doorn, Linda F van Dyk, Marjolein van Egmond, Leo A van Grunsven, Peter Vandenabeele, Wim P Vandenberghe, Ilse Vanhorebeek, Eva C Vaquero, Guillermo Velasco, Tibor Vellai, Jose Miguel Vicencio, Richard D Vierstra, Miquel Vila, Cécile Vindis, Giampietro Viola, Maria Teresa Viscomi, Olga V Voitsekhovskaja, Clarissa von Haefen, Marcela Votruba, Keiji Wada, Richard Wade-Martins, Cheryl L Walker, Craig M Walsh, Jochen Walter, Xiang-Bo Wan, Aimin Wang, Chenguang Wang, Dawei Wang, Fan Wang, Fen Wang, Guanghui Wang, Haichao Wang, Hong-Gang Wang, Horng-Dar Wang, Jin Wang, Ke Wang, Mei Wang, Richard C Wang, Xinglong Wang, Xuejun Wang, Ying-Jan Wang, Yipeng Wang, Zhen Wang, Zhigang Charles Wang, Zhinong Wang, Derick G Wansink, Diane M Ward, Hirotaka Watada, Sarah L Waters, Paul Webster, Lixin Wei, Conrad C Weihl, William A Weiss, Scott M Welford, Long-Ping Wen, Caroline A Whitehouse, J Lindsay Whitton, Alexander J Whitworth, Tom Wileman, John W Wiley, Simon Wilkinson, Dieter Willbold, Roger L Williams, Peter R Williamson, Bradly G Wouters, Chenghan Wu, Dao-Cheng Wu, William K K Wu, Andreas Wyttenbach, Ramnik J Xavier, Zhijun Xi, Pu Xia, Gengfu Xiao, Zhiping Xie, Zhonglin Xie, Da-zhi Xu, Jianzhen Xu, Liang Xu, Xiaolei Xu, Ai Yamamoto, Akitsugu Yamamoto, Shunhei Yamashina, Michiaki Yamashita, Xianghua Yan, Mitsuhiro Yanagida, Dun-Sheng Yang, Elizabeth Yang, Jin-Ming Yang, Shi Yu Yang, Wannian Yang, Wei Yuan Yang, Zhifen Yang, Meng-Chao Yao, Tso-Pang Yao, Behzad Yeganeh, Wei-Lien Yen, Jia-jing Yin, Xiao-Ming Yin, Ook-Joon Yoo, Gyesoon Yoon, Seung-Yong Yoon, Tomohiro Yorimitsu, Yuko Yoshikawa, Tamotsu Yoshimori, Kohki Yoshimoto, Ho Jin You, Richard J Youle, Anas Younes, Li Yu, Long Yu, Seong-Woon Yu, Wai Haung Yu, Zhi-Min Yuan, Zhenyu Yue, Cheol-Heui Yun, Michisuke Yuzaki, Olga Zabirnyk, Elaine Silva-Zacarin, David Zacks, Eldad Zacksenhaus, Nadia Zaffaroni, Zahra Zakeri, Herbert J Zeh 3rd, Scott O Zeitlin, Hong Zhang, Hui-Ling Zhang, Jianhua Zhang, Jing-Pu Zhang, Lin Zhang, Long Zhang, Ming-Yong Zhang, Xu Dong Zhang, Mantong Zhao, Yi-Fang Zhao, Ying Zhao, Zhizhuang J Zhao, Xiaoxiang Zheng, Boris Zhivotovsky, Qing Zhong, Cong-Zhao Zhou, Changlian Zhu, Wei-Guo Zhu, Xiao-Feng Zhu, Xiongwei Zhu, Yuangang Zhu, Teresa Zoladek, Wei-Xing Zong, Antonio Zorzano, Jürgen Zschocke, Brian Zuckerbraun

    Autophagy   8 ( 4 )   445 - 544   2012.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Taylor & Francis  

    In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.

    DOI: 10.4161/auto.19496

    PubMed

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  • The Rab GTPase RabG3b functions in autophagy and contributes to tracheary element differentiation in Arabidopsis Reviewed

    Kwon, SI, Cho, HJ, Jung, JH, Yoshimoto, K, Shirasu, K, Park, OK

    Plant J.   64 ( 1 )   151 - 164   2010.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/j.1365-313X.2010.04315.x

    Web of Science

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  • Advances of autophagy research in plant Invited

    Yoshimoto Kohki

    Regulation of Plant Growth & Development   45 ( 1 )   24 - 32   2010.5

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:The Japanese Society for Chemical Regulation of Plants  

    Plants have to adapt to drastic changes in environmental conditions because of their immortality. They have to cope with various types of environmental stresses, such as starvation, oxidative stress, drought stress, and invasion by phytopathogens, during their differentiation, development and aging processes. The germination and growth of seeds in nitrogen-poor soil or in the shade, for instance, indicates that plants have mechanisms for coping with nitrogen- and carbon-starvation. Under such nutrient-poor conditions the bulk degradation and recycling of macromolecules is integral to the ability of plants to adapt to their environment. Autophagy is the major system responsible for the degradation of organelles, fragmented organelles and cytosolic macromolecules in the vacuole and therefore is assumed to be an extremely important function in plants. Here we briefly describe early studies of plant autophagy, summarize the recent studies on molecular functions of ATG genes and plant autophagy-monitoring systems, and finally introduce the speculated role of autophagy in plants.

    DOI: 10.18978/jscrp.45.1_24

    CiNii Research

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  • Autophagy in plants and phytopathogens Invited Reviewed

    Yoshimoto, K, Takano, Y, Sakai, Y

    FEBS Lett.   584 ( 7 )   1350 - 1358   2010.4

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    DOI: 10.1016/j.febslet.2010.01.007

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  • Role of chloroplasts and other plastids in ageing and death of plants and animals: A tale of Vishnu and Shiva Reviewed

    van Doorn, WG, Yoshimoto, K

    Ageing Res. Rev.   9 ( 2 )   117 - 130   2010.4

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    DOI: 10.1016/j.arr.2009.08.003

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  • Plant autophagy puts the brakes on cell death by controlling salicylic acid signaling Invited Reviewed

    Yoshimoto, K

    Autophagy   6 ( 1 )   192 - 193   2010.1

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    DOI: 10.4161/auto.6.1.10843

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  • Physiological roles of autophagy in plants: Does plant autophagy have a pro-death function? Invited Reviewed

    Yoshimoto, K

    Plant Signal. Behav.   5 ( 5 )   494 - 496   2010

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    DOI: 10.4161/psb.10946

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  • Autophagy negatively regulates cell death by controlling NPR1-dependent salicylic acid signaling during senescence and the innate immune response in Arabidopsis Reviewed

    Yoshimoto, K, Jikumaru, Y, Kamiya, Y, Kusano, M, Consonni, C, Panstruga, R, Ohsumi, Y, Shirasu, K

    Plant Cell   21 ( 9 )   2914 - 2927   2009.9

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    DOI: 10.1105/tpc.109.068635

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  • The role of plant autophagy in nutrient starvation and aging Reviewed

    Yoshimoto Kohki, Ishida Hiroyuki, Wada Shinya, Ohsumi Yoshinori, Shirasu Ken

    Autophagy   5 ( 6 )   904   2009.8

  • Autophagy plays a role in chloroplast degradation during senescence in individually darkened leaves Reviewed

    Wada, S, Ishida, H, Izumi, M, Yoshimoto, K, Ohsumi, Y, Mae, T, Makino, A

    Plant Physiol.   149 ( 2 )   885 - 893   2009.2

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    DOI: 10.1104/pp.108.130013

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  • OsATG10b, an autophagosome component, is needed for cell survival against oxidative stresses in rice Reviewed

    Shin, JH, Yoshimoto, K, Ohsumi, Y, Jeon, JS, An G

    Mol. Cells   27 ( 1 )   67 - 74   2009.1

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    DOI: 10.1007/s10059-009-0006-2

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  • Chloroplasts are partially mobilized to the vacuole by autophagy Reviewed

    Ishida, H, Yoshimoto, K

    Autophagy   4 ( 7 )   961 - 962   2008.10

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  • Mobilization of rubisco and stroma-localized fluorescent proteins of chloroplasts to the vacuole by an ATG gene-dependent autophagic process Reviewed

    Ishida, H, Yoshimoto, K, Izumi, M, Reisen, D, Yano, Y, Makino, A, Ohsumi, Y, Hanson, MR, Mae, T

    Plant Physiol.   148 ( 1 )   142 - 155   2008.9

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    DOI: 10.1104/pp.108.122770

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  • Visualization of Rubisco-containing bodies derived from chloroplasts in living cells of Arabidopsis. Reviewed

    Ishida, H, Yoshimoto, K, Reisen, D, Makino, A, Ohsumi, Y, Hanson, M.R, Mae, T

    Photosynthesis. Energy from the sun   1207 - 1210   2008.9

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  • In vitro reconstitution of plant ATG8 and ATG12 conjugation systems essential for autophagy Reviewed

    Fujioka, Y, Noda, NN, Fujii, K, Yoshimoto, K, Ohsumi, Y, Inagaki, F

    J. Biol. Chem.   283 ( 4 )   1921 - 1928   2008.1

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    DOI: 10.1074/jbc.M706214200

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  • An Arabidopsis homolog of yeast ATG6/VPS30 is essential for pollen germination Reviewed

    Fujiki, Y, Yoshimoto, K, Ohsumi, Y

    Plant Physiol.   143 ( 3 )   1132 - 1139   2007.3

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    DOI: 10.1104/pp.106.093864

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  • AtATG genes, homologs of yeast autophagy genes, are involved in constitutive autophagy in Arabidopsis root tip cells Reviewed

    Inoue, Y, Suzuki, T, Hattori, M, Yoshimoto, K, Ohsumi, Y, Moriyasu, Y

    Plant Cell Pysiol.   47 ( 12 )   1641 - 1652   2006.12

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    DOI: 10.1093/pcp/pcl031

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  • Autophagy in higher plant Invited

    Yoshimoto Kohki

    Denshi kenbikyo   41 ( 2 )   84 - 86   2006.4

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    DOI: 10.11410/kenbikyo2004.41.84

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  • Autophagy in development and stress responses of plants Reviewed

    Bassham, DC, Laporte, M, Marty, F, Moriyasu, Y, Ohsumi, Y, Olsen, LJ, Yoshimoto, K

    Autophagy   2 ( 1 )   2 - 11   2006.1

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  • The crystal structure of plant ATG12 and its biological implication in autophagy Reviewed

    Suzuki, NN, Yoshimoto, K, Fujioka, Y, Ohsumi, Y, Inagaki, F

    Autophagy   1 ( 2 )   119 - 126   2005.7

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  • 植物のオートファジー Invited

    吉本光希

    細胞工学   24 ( 6 )   593 - 596   2005.5

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  • Processing of ATG8s, ubiquitin-like proteins, and their deconjugation by ATG4s are essential for plant autophagy Reviewed

    Yoshimoto, K, Hanaoka, H, Sato, S, Kato, T, Tabata, S, Noda, T, Ohsumi, Y

    Plant Cell   16 ( 11 )   2967 - 2983   2004.11

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    DOI: 10.1105/tpc.104.025395

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  • オートファジー: 植物細胞の液胞とオートファジー Invited

    吉本光希

    生体の科学   54   534 - 539   2003.12

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    DOI: 10.11477/mf.2425100798

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  • A novel selection method based on the expression level of green fluorescent protein measured with a quantitative fluorescence imager Reviewed

    Niwa, Y, Yoshimoto, K, Goto, S, Kuruto-Niwa, R

    Plant Biotechnol.   20 ( 2 )   165 - 168   2003

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    DOI: 10.5511/plantbiotechnology.20.165

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  • Comparison of strength of endogenous and exogenous gene promoters in Arabidopsis chloroplasts Reviewed

    Yoshimoto, K, Sakaiya, M, Isono, K, Kobayashi, H

    Plant Biotechnol.   18 ( 2 )   135 - 142   2001

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    DOI: 10.5511/plantbiotechnology.18.135

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  • 葉緑体の転写調節:光合成機能発現制御機構 Invited

    清水正則, 吉本光希, 小林裕和

    蛋白質核酸酵素   45   123 - 131   2000.8

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  • Transcriptional regulation in the chloroplast: mechanisms of coordinated expression of photosynthesis genes

    M. Shimizu, K. Yoshimoto, H. Kobayashi

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   45   123 - 131   2000.1

  • Non-invasive quantitative detection and applications of non-toxic, S65T-type green fluorescent protein in living plants Reviewed

    Niwa, Y, Hirano, T, Yoshimoto, K, Shimizu, M, Kobayashi, H

    Plant J.   18 ( 4 )   455 - 463   1999.5

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    DOI: 10.1046/j.1365-313X.1999.00464.x

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  • Several strategies for dissecting and controlling functions in plant cells Reviewed

    H Kobayashi, K Yoshimoto, M Sakaiya, Y Narusaka, J Sheen, Y Niwa

    MOLECULAR GENETICS OF HOST-SPECIFIC TOXINS IN PLANT DISEASES   13   399 - 400   1998

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  • Leaf-specifically expressed genes for polypeptides destined for chloroplasts with domains of sigma(70) factors of bacterial RNA polymerases in Arabidopsis thaliana Reviewed

    Isono, K, Shimizu, M, Yoshimoto, K, Niwa, Y, Satoh, K, Yokota, A, Kobayashi, H

    Proc. Natl. Acad. Sci. USA.   94 ( 26 )   14948 - 14953   1997.12

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    DOI: 10.1073/pnas.94.26.14948

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Books

  • milsil(ミルシル) 特集:細胞内の分解システム オートファジー

    吉本光希( Role: Joint author植物特有のオートファジーによる生き残り戦略)

    国立科学博物館  2025.12 

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    Responsible for pages:12-14   Book type:General book, introductory book for general audience

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  • 植物オートファジーの生理的意義 〜植物独自の機能の解明を目指して〜

    篠崎大樹, 井上和也, 吉本光希( Role: Joint author)

    日本植物学会  2018.9 

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    Responsible for pages:3-10   Language:Japanese   Book type:Scholarly book

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  • 植物オートファジー研究の第二の夜明け

    吉本光希, 朽津和幸( Role: Joint author)

    日本植物学会  2018.9 

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    Responsible for pages:1-2   Language:Japanese   Book type:Scholarly book

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  • オートファジー ~分子メカニズムの理解から病態の解明まで~

    吉本光希( Role: Joint author植物のさまざまな局面におけるオートファジーの生理機能)

    南山堂  2018.1 

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  • 植物学の百科事典

    吉本光希( Role: Joint author生理学:植物の老化)

    丸善出版  2016.6 

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  • Photosynthesis. Energy from the sun

    Ishida, H., Yoshimoto, K., Reisen, D., Makino, A., Ohsumi, Y., Hanson, M.R., and Mae, T.( Role: Joint authorVisualization of Rubisco-containing bodies derived from chloroplasts in living cells of Arabidopsis.)

    Springer  2008.1 

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    Total pages:1640   Language:English   Book type:Scholarly book

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  • ユビキチン-プロテアソーム系とオートファジー; 作動機構と病態生理

    吉本光希( Role: Joint author植物オートファジー研究の新展開:植物のatg変異体)

    共立出版  2007.2 

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    Total pages:pp.1119-1536   Language:Japanese   Book type:Scholarly book

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Presentations

  • オートファジー関連 ATG9 小胞局在タンパク質の解析

    只木亮哉, 黒崎智史, 井上和也, 篠崎大樹, 吉本光希

    日本植物学会第89回大会  2025.9 

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  • Autophagy-mediated quality control of a photoreceptor during seed development in Arabidopsis Invited

    Seta, K., Yoshimoto, K.

    Joint Meeting of Japan Society for Cell Biology 77th and Japanese Society of Developmental Biologists 58th  2025.7 

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  • ヒメツリガネゴケにおいてオートファジー欠損株で誘導される早期老化の解析

    井上悠子, 森安裕二, 吉本光希

    第66回日本植物生理学会大会  2025.3 

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  • 接木は組織修復においてオートファジーを誘導する

    黒谷賢一, 篠崎大樹, 田畑亮, 川勝弥一, 杉田亮平, 岡田健太郎, 宇津木優樹, 岡安浩次, 森萌, 田野井慶太朗, 豊岡公徳, 吉本光希, 野田口理孝

    第66回日本植物生理学会大会  2025.3 

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  • 植物オートファジーの新規分子機構解明に向けたオートファジー関連ATG9小胞の解析

    只木亮哉, 黒崎智史, 井上和也, 篠崎大樹, 吉本光希

    日本植物学会第88回大会  2024.9 

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  • 接木は組織修復においてオートファジーを誘導する

    黒谷賢一, 篠崎大樹, 田畑亮, 川勝弥一, 杉田亮平, 岡田健太郎, 宇津木優樹, 岡安浩次, 森萌, 田野井慶太朗, 吉本光希, 野田口理孝

    日本植物学会第88回大会  2024.9 

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  • 光制限下でみえてきたシロイヌナズナの種子発芽におけるオートファジーの役割

    瀬田京介, 吉竹悠宇志, 吉本光希

    日本植物学会第88回大会  2024.9 

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  • ミクロオートファジーにおける液胞膜局在ATG2・SGR2タンパク質の機能解析

    柳生真子, 吉本光希

    日本植物学会第88回大会  2024.9 

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  • ヒメツリガネゴケのオートファジー欠損株が窒素欠乏条件下で早期老化する機構の解析

    井上悠子, 森安裕二, 吉本光希

    日本植物学会第88回大会  2024.9 

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  • 長期保存種子の発芽能力維持におけるオートファジーの重要性

    篠崎大樹, 高山恵莉菜, 川上直人, 吉本光希

    第65回日本植物生理学会年会  2024.3 

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  • リン酸欠乏時にストリゴラクトンはストロミュール形成およびクロロファジーを制御する

    吉竹悠宇志, 吉本光希

    第65回日本植物生理学会年会  2024.3 

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  • オートファジーはシロイヌナズナの種子発芽に寄与する

    瀬田京介, 吉本光希

    日本植物学会第87回大会  2023.9 

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  • オートファジーによる分解産物のアミノ酸が,ストレス条件下での老化とオートファジー継続に及ぼす影響

    井上悠子, Akter Most Mohoshena, 森安裕二, 吉本光希

    日本植物学会第87回大会  2023.9 

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  • 唯一のオートファジー関連膜タンパク質 ATG9の局在解析

    只木亮哉, 黒崎智史, 井上和也, 篠崎大樹, 吉本光希

    日本植物学会第87回大会  2023.9 

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  • ミクロオートファジーにおける液胞膜局在

    柳生真子, 吉本光希

    日本植物学会第87回大会  2023.9 

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  • 葉緑体局在DPD1エキソヌクレアーゼ欠損変異体ではクロロファジーが抑制される

    吉竹悠宇志, 吉本光希

    第64回日本植物生理学会年会  2023.3 

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  • Excess supply of nitrate induces chlorophagy under phosphate starvation in plants Invited

    Yoshitake Y, Yoshimoto K

    The 10th International Symposium on Autophagy  2022.10 

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  • 植物リン酸リサイクルにおける膜脂質転換とオートファジー

    吉竹悠宇志, 吉本光希

    第34回植物脂質シンポジウム  2022.9 

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  • 細胞内自己成分分解系オートファジーが担う植物体内亜鉛ホメオスタシス Invited

    篠崎大樹, 吉本光希

    第33回日本微量元素学会学術集会  2022.9 

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  • 植物体内 Zn-Fe イオンバランス維持における自己成分分解系 オートファジーの重要性 Invited

    吉本光希

    令和4年度 新学術領域 「生命金属科学」 夏の合宿  2022.9 

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  • 小胞体ストレス誘導性のオートファジーは早期リン酸欠乏下でのリン酸再供給に関与する

    吉竹悠宇志, 篠崎大樹, 吉本光希

    第63回日本植物生理学会年会  2022.3 

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  • マクロオートファジー関連タンパク質ATG2のミクロオートファジーにおける機能解析

    柳生真子, 吉本光希

    第63回日本植物生理学会年会  2022.3 

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  • 植物における液胞膜動態を介する自己分解機構 “ミクロ”オートファジー関連因子の探索

    柳生真子, 吉本光希

    第44回日本分子生物学会  2021.12 

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  • 植物ミクロオートファジー関連因子の探索 Invited

    吉本光希

    第14回オートファジー研究会  2021.10 

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  • 植物の早期リン酸欠乏応答機構の解析

    吉竹悠宇志, 吉本光希

    第33回植物脂質シンポジウム  2021.9 

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  • 植物におけるミクロオートファジー関連因子の探索

    柳生真子, 吉本光希

    日本植物学会 第85回大会  2021.9 

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  • オートファジーの植物体内金属恒常性維持機構としての新たな一面

    篠崎大樹, 吉本光希

    日本植物学会 第85回大会  2021.9 

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  • オートファジーと概日時計間の相互作用の解析

    齋藤由花, 藤森梢, 吉本光希

    日本植物学会 第85回大会  2021.9 

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  • Importance of autophagy in phosphate recycle system

    2021.3 

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  • アンモニアストレス下におけるマクロ・ミクロオートファジーの膜動態 Invited

    吉本光希

    第93回日本生化学会大会  2020.9 

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    Venue:オンライン(Zoom)  

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  • オートファジーによる亜鉛の再利用効率向上は,亜鉛欠乏時の葉緑体でのヒドロキシラジカルの生成を抑制する

    篠崎大樹, Ekaterina Merkulova, Loreto Naya, Celine Masclaux-Daubresse, 吉本光希

    第61回日本植物生理学会年会  2020.3 

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  • 主根伸長における小胞体局在SNAREタンパク質の役割

    吉竹悠宇志, 早坂渉, 吉本光希

    第61回日本植物生理学会年会  2020.3 

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  • 選択的・非選択的オートファジーによる植物生体内恒常性維持 Invited

    吉本光希

    第22回植物オルガネラワークショップ  2020.3 

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  • Physiological roles of autophagy under Zn deficiency in plants Invited International conference

    Yoshimoto, K

    The 7th International Conference on Food Factors  2019.12 

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    Venue:Kobe  

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  • 様々なタイプのオートファジーによる植物の高次機能発現

    吉本光希

    第12回オートファジー研究会  2019.10 

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    Venue:つま恋リゾート彩の郷(静岡)  

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  • カルス形成におけるオートファジーの役割

    宇津木優樹, 岩瀬哲, 吉本光希

    第12回オートファジー研究会  2019.10 

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    Venue:つま恋リゾート彩の郷(静岡)  

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  • 植物オートファジーによる亜鉛の生物的利用能の向上

    篠崎 大樹, Ekaterina A. Merkulova, Loreto Naya, 堀江 哲郎, 大隅 良典, Céline Masclaux-Daubresse, 吉本 光希

    第5回植物の栄養研究会  2019.9  植物の栄養研究会

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    Language:Japanese   Presentation type:Poster presentation  

    Venue:広島大学(広島)  

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  • Plant autophagy: an intracellular system for bulk and selective self-degradation Invited International conference

    Yoshimoto, K

    Japan-Taiwan Plant Biology 2019  2019.3  Japanese Society of Plant Physiologists and Taiwan Society of Plant Biologists

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    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Nagoya University (Nagoya)  

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  • カルス形成におけるオートファジーの役割

    宇津木優樹, 岩瀬哲, 吉本光希

    第60回日本植物生理学会年会  2019.3  日本植物生理学会

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    Language:Japanese   Presentation type:Poster presentation  

    Venue:名古屋大学(名古屋)  

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  • Physiological meanings of plant autophagy in response to environmental stresses. Aiming to elucidate plant specific functions of autophagy Invited International conference

    Yoshimoto, K

    XVI Latin American Plant Physiology Congress  2018.11 

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    Venue:Universidad Nacional de Cordoba (Cordoba, Argentina)  

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  • 植物の様々な局面におけるオートファジーの重要性 Invited

    吉本光希

    日本アミノ酸学会 第12回学術大会  2018.10  日本アミノ酸学会

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    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:明治大学(東京)  

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  • 植物の亜鉛欠乏応答におけるオートファジーの役割

    篠崎大樹, Ekaterina Merkulova, Loreto Naya, Celine Masclaux-Daubresse, 吉本光希

    日本植物学会第82回大会  2018.9  日本植物学会

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    Language:Japanese   Presentation type:Poster presentation  

    Venue:広島国際会議場、広島  

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  • オートファジーによる緑葉ペルオキシソーム選択機構の解明

    井上和也, Loreto Naya, 佐藤繭子, 豊岡公徳, 吉本光希

    日本植物学会第82回大会  2018.9  日本植物学会

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:広島国際会議場(広島)  

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  • 植物の亜鉛欠乏応答におけるオートファジーの役割

    篠崎大樹, Ekaterina Merkulova, Loreto Naya, Celine Masclaux-Daubresse, 吉本光希

    第4回植物の栄養研究会  2018.9  植物の栄養研究会

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    Language:Japanese   Presentation type:Poster presentation  

    Venue:京都大学(京都)  

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  • Molecular mimicry: Parasitic Strategy of Bursaphelenchus xylophilus

    2018.9 

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  • オートファジーは種子の発芽勢に重要である

    高山恵莉菜, 吉本光希, 川上直人

    第59回日本植物生理学会年会  2018.3 

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    Language:Japanese   Presentation type:Poster presentation  

    Venue:札幌コンベンションセンター、札幌  

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  • セレクティブオートファジーによる緑葉ペルオキシソームの品質管理 Invited

    吉本 光希

    第59回日本植物生理学会年会シンポジウム  2018.3 

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    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:札幌コンベンションセンター、札幌  

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  • 亜鉛欠乏応答における植物オートファジーの重要性

    篠崎大樹, Ekaterina Merkulova, Loreto Naya, Celine Masclaux-Daubresse, 吉本光希

    第59回日本植物生理学会年会  2018.3 

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    Language:Japanese   Presentation type:Poster presentation  

    Venue:札幌コンベンションセンター(札幌)  

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  • 選択的オートファジーによる緑葉ペルオキシソームの分解機構

    井上和也, Loreto Naya, 佐藤繭子, 豊岡公徳, 吉本光希

    第59回日本植物生理学会年会  2018.3 

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    Language:Japanese   Presentation type:Poster presentation  

    Venue:札幌コンベンションセンター(札幌)  

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  • マツノゼイサンチュウ分泌タンパク質のin planta機能解析

    桐野巴瑠, 吉本光希, 新屋良治

    第129回日本森林学会大会  2018.3 

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    Language:Japanese   Presentation type:Poster presentation  

    Venue:高知大学(高知)  

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  • 植物ミクロオートファジー ~液胞内分解系のバックアップシステムとして~ Invited

    吉本光希

    日本農芸化学会2018年度大会シンポジウム  2018.3 

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    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:名城大学(名古屋)  

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  • 分子擬態ー似せて騙す線虫の寄生戦略

    桐野巴瑠, 吉本光希, 新屋良治

    第62回日本応用動物昆虫学会大会  2018.3 

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    Venue:鹿児島大学(鹿児島)  

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  • Physiological meaning of plant autophagy in response to environmental stresses Invited

    Kohki Yoshimoto, Daiki Shinozaki, Kazuya Inoue, Ekaterina A. Merkulova, Céline Masclaux-Daubresse

    Bilateral Closure Symposium of GDRI Integrative Plant Biology Network Program "The developing plant in its environment"  2017.10  GDRI-IPB

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    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:Domaine Lyon Saint-Joseph(Lyon, France)  

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  • アンモニウムストレスは2つのオートファジー過程に真逆の影響を与える Invited

    吉本光希

    日本植物学会第81回大会  2017.9  日本植物学会

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    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:東京理科大(野田)  

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  • 植物ペキソファジー:器官特異的な植物ペルオキシソーム分解によるオルガネラ品質管理機構 Invited

    吉本光希

    39回 日本分子生物学会年会  2016.11  日本分子生物学会

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    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:パシフィコ横浜(横浜)  

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  • Adaptation of metabolism in autophagy-defective plants during environmental stresses Invited

    2016.3 

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  • Organelle degradation by autophagy, an intracellular self-degradation system Invited International conference

    11th International Congress of Plant Molecular Biology  2015.10 

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    Language:English   Presentation type:Symposium, workshop panel (nominated)  

    Venue:Bourbon Cataratas Convention & Spa Resort (Iguazu Falls, Brazil)  

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  • 細胞内自己分解システム・オートファジーによる選択的オルガネラ分解 Invited

    吉本光希

    第55回 日本植物生理学会年会  2014.3  日本植物生理学会

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    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:富山大学(富山)  

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  • Plant autophagy negatively regulates cell death by controlling salicylic acid signaling during senescence and the innate immune response International conference

    The10th Gordon research conference:Plant senescence  2012.7 

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    Venue:Stonehill College (USA, Massachusetts)  

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  • 細胞内自己分解システム・オートファジーの分子機構とその生理機能に関する研究 Invited

    吉本光希

    第53回 日本植物生理学会年会  2012.3  日本植物生理学会

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    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:京都産業大学(京都)  

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  • 植物オートファジーによる細胞死の制御 Invited

    吉本光希

    第52回 日本植物生理学会年会  2011.3  日本植物生理学会

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    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:東北大学(仙台)  

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  • New insights into the physiological role of autophagy in plants.

    Yoshimoto, K

    Symposium Perspective of Plant Science 2010  2010.11 

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    Venue:Okazaki Conference Center (Okazaki)  

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  • 老化・病原菌感染時における植物オートファジーの役割

    吉本光希, 大隅良典, 白須賢

    第74回 日本植物学会大会  2010.9  日本植物学会大会

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:中部大学(春日井)  

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  • Plant autophagy negatively regulates cell death by controlling salicylic acid signaling during senescence and the innate immune response Invited International conference

    Yoshimoto, K

    21st International Conference on Arabidopsis Research  2010.6 

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    Venue:Pacifico Yokohama (Yokohama)  

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  • オートファジー能欠損シロイヌナズナにおけるプログラム細胞死の原因は過剰なサリチル酸シグナリングである

    吉本光希, 大隅良典, 白須賢

    平成22年度日本植物病理学会大会  2010.4  日本植物病理学会

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:国立京都国際会館(京都)  

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  • The role of plant autophagy in nutrient starvation and aging Invited

    Yoshimoto, K

    5th International Symposium on Autophagy  2009.9 

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    Venue:Ohtsu Prince Hotel (Shiga)  

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  • オートファジー能欠損シロイヌナズナにおけるプログラム細胞死と病原菌抵抗性反応

    吉本光希, 大隅良典, 白須賢

    平成21年度日本植物病理学会大会  2009.3  日本植物病理学会

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:山形大学(山形)  

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  • 植物オートファジーの新たな生理的役割

    吉本光希, 大隅良典

    第50回 日本植物生理学会年会  2009.3  日本植物生理学会

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋大学(名古屋)  

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  • 植物オートファジーと細胞死の制御 Invited

    吉本光希

    第31回 日本分子生物学会年会・第81回 日本生化学会大会 合同大会  2008.12 

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    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:神戸ポートアイランド(神戸)  

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  • 高等植物におけるオートファジーの生理的役割

    吉本光希, 大隅良典

    第47回 日本植物生理学会年会  2006.3  日本植物生理学会

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:筑波大学(筑波)  

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  • 植物においてオートファジーが果たす生理学的役割 Invited

    吉本光希

    第28回 日本分子生物学会年会  2005.12  日本分子生物学会

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    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:ヤフードーム(福岡)  

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  • Physiological roles of plant autophagy Invited

    Yoshimoto, K

    第78回 日本生化学会大会  2005.10  日本生化学会

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    Language:English   Presentation type:Symposium, workshop panel (nominated)  

    Venue:神戸ポートアイランド(神戸)  

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  • 高等植物のオートファジー:オートファジー欠損植物は早く老化する Invited

    吉本光希

    第46回 日本植物生理学会年会  2005.3  日本植物生理学会

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    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:新潟コンベンションセンター(新潟)  

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  • Plant autophagy: Autophagy-defective mutants exhibited early senescence Invited International conference

    The 8th Gordon research conference: Plant senescence  2004.6 

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    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Mount Holyoke College (USA, Massachusetts)  

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  • 植物オートファジーにおけるAtATG8ファミリーの解析

    吉本光希, 花岡秀樹, 佐藤修正, 加藤友彦, 田畑哲之, 野田健司, 大隅良典

    第45回 日本植物生理学会年会  2004.3  日本植物生理学会

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京都立大学(東京)  

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  • 植物オートファジーにおけるAtATG8ファミリーの解析

    吉本光希, 花岡秀樹, 野田健司, 佐藤修正, 加藤友彦, 田畑哲之, 大隅良典

    第44回 日本植物生理学会年会  2003.3  日本植物生理学会

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:近畿大学(大阪)  

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  • 葉緑体形質転換系の現状と課題 Invited

    小林裕和, 吉本光希

    第42回 日本植物生理学会年会  2001.3  日本植物生理学会

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    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:九州産業大学(福岡)  

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  • シロイヌナズナにおける3種類のσ因子は葉緑体ゲノムにコードされる異なる光合成遺伝子のそれぞれの転写を促進する

    吉本光希, 清水正則, 磯野協一, 小林裕和

    第40回 日本植物生理学会年会  1999.3  日本植物生理学会

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東北大学(仙台)  

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  • シロイヌナズナ葉緑体における一過性発現による遺伝子プロモーター強度の評価

    吉本光希, 境谷真男, 磯野協一, 小林裕和

    第38回 日本植物生理学会年会  1997.3  日本植物生理学会

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:京都大学(京都)  

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Awards

  • 連合駿台会 学術賞

    2025   連合駿台会  

    吉本光希

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  • 日本光生物学協会 ポスター賞

    2024   日本光生物学協会  

    瀬田京介, 吉竹悠宇志, 吉本光希

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    Award type:Award from Japanese society, conference, symposium, etc.  Country/Region:Japan

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  • 日本植物形態学会 ポスター賞

    2023   日本植物形態学会  

    只木亮哉, 吉本光希

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    Award type:Award from Japanese society, conference, symposium, etc.  Country/Region:Japan

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  • PCP Top Cited Review Paper Award

    2022  

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    Award type:Honored in official journal of a scientific society, scientific journal  Country/Region:Japan

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  • 植物の栄養研究会 優秀ポスター賞

    2021   植物の栄養研究会  

    齋藤由花, 藤森梢, 吉本光希

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    Award type:Award from Japanese society, conference, symposium, etc.  Country/Region:Japan

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  • 植物の栄養研究会 最優秀ポスター賞

    2019   植物の栄養研究会  

    篠崎大樹, Ekaterina A. Merkulova, Loreto Naya, 堀江哲郎, 大隅良典, Céline Masclaux-Daubresse, 吉本光希

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    Award type:Award from Japanese society, conference, symposium, etc.  Country/Region:Japan

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  • 植物の栄養研究会 最優秀ポスター賞

    2018   植物の栄養研究会  

    篠崎大樹, Ekaterina A. Merkulova, Loreto Naya, Celine Masclaux-Daubresse, 吉本光希

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    Award type:Award from Japanese society, conference, symposium, etc.  Country/Region:Japan

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  • 日本植物生理学会 奨励賞

    2012   日本植物生理学会  

    吉本光希

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    Award type:Award from Japanese society, conference, symposium, etc.  Country/Region:Japan

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  • INRA Package フェローシップ

    2011   フランス国立農学研究所  

    Yoshimoto, K

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    Country/Region:France

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  • 基礎科学特別研究員 フェローシップ

    2007   理化学研究所  

    吉本光希

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Research Projects

  • 植物オートファジーによる光受容体の品質管理 ~その分子基盤と生理的意義の解明~

    2025.11 - 2027.10

    公益財団法人 大隅基礎科学創成財団  研究助成(基礎科学(一般)) 

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  • 細胞内自己成分分解機構・オートファジーを介した新規オルガネラ品質機構の解明

    2025.6 - 2028.3

    明治大学 科学技術研究所 重点研究  重点研究(A)

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  • オートファジー欠損変異体を活用した新規・植物体内亜鉛イオン恒常性維持機構の解明

    2023.4 - 2026.3

    日本学術振興会  基盤研究(B) 

    吉本 光希

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  • Analysis of the mechanism of plastid degradation responding to external nutrients

    Grant number:R501  2023.4 - 2024.3

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  • Analysis of the mechanism of plastid degradation responding to external nutrients

    Grant number:R439  2022.4 - 2023.3

    the Joint Usage/Research Center, Institute of Plant Science and Resources, Okayama University 

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  • ユビキチン様オートファジー蛋白質との相互作用を介した異常オルガネラ認識機構

    2020.4 - 2023.3

    日本学術振興会  基盤研究(B) 

    吉本 光希

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  • 様々なタイプのオートファジーによる植物の高次機能発現

    2019.6 - 2024.3

    日本学術振興会  新学術領域研究(研究領域提案型)計画班 

    吉本 光希、海老根 一生

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  • 独立栄養生物特異的な選択的オートファジーの分子機構の解明

    2017.7 - 2020.3

    明治大学 科学技術研究所 重点研究  重点研究(A)

    吉本 光希

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  • オートファジー破綻植物を用いた順遺伝学による新規暗所誘導型細胞死因子の単離・同定

    2016.6 - 2018.3

    日本学術振興会  研究活動スタート支援 

    吉本 光希

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  • 環境応答機能の解明に基づく高度環境適応植物デザイン研究基盤の確立

    2016.4 - 2019.3

    私立大学 研究基盤形成支援事業  私立大学 研究基盤形成支援事業

    川上 直人 教授(明治大学)

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    Authorship:Coinvestigator(s)  Grant type:Competitive

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  • Role of autophagy in plant adaptation to nitrate and sulfate limitaion in environment

    2012.9 - 2016.9

    Dr.Celine Masclaux-Daubress (INRA Versailles)

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    Authorship:Coinvestigator(s) 

    Grant amount:\66000000 ( Direct Cost: \66000000 )

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  • Clever survival strategy for plants: selective degradation of organelle/proteins via autophagy in response to environmental stresses

    2011.12 - 2015.12

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    Grant amount:\74000000 ( Direct Cost: \74000000 )

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  • Elucidation of mechanisms of selective peroxisome degradation using an ubiquitin-like autophagy protein

    Grant number:22770049  2010.4 - 2012.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (B)

    YOSHIMOTO Koki

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    Authorship:Principal investigator 

    Grant amount:\3300000 ( Direct Cost: \3300000 )

    Autophagy is the major system responsible for the degradation of organelles and cytosolic macromolecules in the vacuole. I found that dysfunctional peroxisomes were highly accumulated in leaves of autophagy-defective mutants, indicating that autophagy is involved in quality control of leaf peroxisomes in plants. I also tried to elucidate molecular mechanisms for selective peroxisome degradation in Arabidopsis.

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  • Role of TOR in the regulation of autophagy and nitrogen recycling in plants

    2009.4 - 2011.3

    日本学術振興会  二国間交流事業  共同研究(SAKURAプログラム)

    佐藤文彦教授(京都大学)

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    Authorship:Coinvestigator(s) 

    Grant amount:\1800000 ( Direct Cost: \1800000 )

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  • An ingenious strategy for plant survival : Degradation of chloroplast proteins via autophagy and RCB vesicles

    Grant number:20200061  2008.11 - 2011.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)

    ISHIDA Hiroyuki, YOSIMOTO Kohki

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    Authorship:Coinvestigator(s) 

    Grant amount:\23900000 ( Direct Cost: \23900000 )

    We studied mechanisms and physiological functions of a degradation of chloroplast proteins by autophagy as a strategy of plant survival. It was revealed that (i) tye production of RCBs which contain stromal proteins of chloroplasts is dependent on autophagy; (ii) the RCB production is closely related to leaf carbon status; (iii) autophagy negatively regulates leaf senescence by controlling salicylic acid signaling and the production of reactive oxygen species.

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  • Investigation of a novel cell-death activator in autophagy-defective mutants

    2007.4 - 2010.3

    理化学研究所  基礎科学特別研究員 

    吉本 光希

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    Authorship:Principal investigator 

    Grant amount:\3900000 ( Direct Cost: \3900000 )

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  • 高等植物におけるオートファジーの生理的役割

    Grant number:19039033  2007.4 - 2009.3

    日本学術振興会  特定領域研究  特定領域研究

    鈴木邦律 助教(基礎生物学研究所)

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    Authorship:Coinvestigator(s) 

    Grant amount:\4600000 ( Direct Cost: \4600000 )

    オートファジーとは, 細胞質成分を細胞内分解コンパートメントである液胞に輸送して分解する, 真核細胞に普遍的な細胞内分解システムである。我々は, シロイヌナズナにおいてオートファジーに必須なATG(autophagy-related)遺伝子群の果たす役割を解析し, (1)オートファジーが老化の抑制に関与していること, (2)オートファジー能欠損植物では, 病原菌感染時におこる過敏感反応細胞死の進行が早いこと, (3)ATG6遺伝子の変異が雄性不稔の表現型を示すことを示してきた.
    老化と病原体抵抗性に関わっていると言われているサリチル酸に焦点を当て, その関与を検討した. サリチル酸をカテコールに転換する酵素遺伝子NahGを過剰発現させた植物, あるいはサリチル酸生合成, サリチル酸シグナリング系のmutantとの二重変異体ではオートファジー不能植物の老化促進表現型が抑制された. 一方で, ジャスモン酸やエチレンのmutantではその表現型が抑制されなかった. また, NahGにより老化促進表現型が抑制された植物にサリチル酸のアナログBTHを添加するとその抑制は解除された節以上の結果から, オートファジー不能植物ではサリチル酸生合成以後のサリチル酸シグナリングが過剰になっていることが示唆された.
    Atg6を含むシロイヌナズナのPI3K複合体の変異体が雄性不稔となるメカニズムの解明を進めた結果, PI3Kがおそらくは活性酸素種(ROS)などを介した花粉発芽のシグナリングを制御する一方, 花粉の発達(液胞形態の維持など)にも一部関与することが明らかとなった. さらに, 花粉特異的プロモーターを使った機能相補により, atg6変異体の雄性不稔を回避してノックアウト植物体を得ることができた.

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  • Identification of a cell death inducible factor in autophagy-defective plants

    Grant number:18770040  2006.4 - 2009.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (B)

    YOSHIMOTO Koki

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    Authorship:Principal investigator 

    Grant amount:\3780000 ( Direct Cost: \3780000 )

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  • 高等植物におけるオートファジーの動態解析

    Grant number:17051031  2005.4 - 2007.3

    日本学術振興会  特定領域研究  特定領域研究

    鈴木邦律 助教(基礎生物学研究所)

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    Authorship:Coinvestigator(s) 

    Grant amount:\4200000 ( Direct Cost: \4200000 )

    AtATG遺伝子破壊株では栄養飢餓条件下に老化が促進されることや根の伸長が阻害されることが明らかとなった(Yoshimoto et al., Pkant Cell, 16, 2967-2983, 2004).この結果はシロイヌナズナにも他の生物種と同様のオートファジーのシステムが保存されており,ATG遺伝子がオートファジーの進行に重要な役割を担っていることを示唆している.そこで,高等植物でオートファジーの進行をモニタリングする系を開発した.出芽酵母や高等動物細胞において,Atg8はオートファジーの可視化マーカーとして知られており,オートファジーの進行に伴って液胞/リソソームに輸送される.GFP-AtATG8融合タンパク質を発現させた形質転換植物を作製したところ,野生型植物において,GFP-AtATG8は細胞質中のリング状構造(オートファゴソーム)に局在し,窒素飢餓条件下で液胞内へと移行した.オートファジー能を欠損した植物においてGFP-AtATG8の挙動を解析したところ,窒素飢餓条件下でもGFP-AtATG8は液胞内に移行しなかった.
    最近になって,ウイルスに対する抵抗性反応の一つ,過敏感反応細胞死(HR-PCD)の現象とオートファジーが密接に関連していることが報告された(Liu et al., Cell, 121, 567-577, 2005).我々もほぼ同時期に植物病原バクテリアPseudomonas syringae pv. tomato感染時にHR-PCDが過剰に起こることを見いだした.
    また,花粉管形成時にオートファジー関連遺伝子AtVPS30遺伝子が重要な機能を果たしていることを明らかにし,論文として発表した(Fujiki et al., Plant Physiol., 143, 1132-1139, 2007).

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  • T7 RNAポリメラーゼ高効率発現系を利用したシロイヌナズナ葉緑体形質転換系の開発

    2000.4 - 2001.3

    浜松科学技術研究振興会 

    吉本 光希

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    Authorship:Principal investigator 

    Grant amount:\100000 ( Direct Cost: \100000 )

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