Updated on 2026/03/10

写真a

 
KUSHIRO TETSUO
 
Organization
Undergraduate School School of Agriculture Professor
Title
Professor
External link

Degree

  • 博士(薬学) ( 東京大学 )

Research Areas

  • Life sciences / Bioorganic chemistry

Education

  • The University of Tokyo   Graduate School of Pharmaceutical Sciences

    1995.4 - 1998.3

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    Country/Region: Japan

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  • Tokyo Institute of Technology   Graduate School, Division of Natural Science

    1993.4 - 1995.3

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    Country/Region: Japan

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  • Tokyo Institute of Technology   Faculty of Science   Department of Chemistry

    1989.4 - 1993.3

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    Country/Region: Japan

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Research History

  • Meiji University   School of Agriculture   Professor

    2020.4

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    Country/Region:Japan

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  • Meiji University   School of Agriculture   Associate Professor

    2010.4 - 2020.3

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  • Assistant Professor

    2005.4 - 2010.3

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  • Researcher

    2002.9 - 2005

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  • Scripps Research Institute Research Associate

    2000.9 - 2002

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  • Special researcher of the Japan Society for the Promotion of Science

    1998.7 - 2000

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Professional Memberships

Papers

  • Genetic analysis of β-carotene isomerase in Arabidopsis thaliana. International journal

    Hitomi Kobuna, Yuri Kanno, Daisuke Fukuhara, Yoshiya Seto, Tetsuo Kushiro, Masanori Okamoto

    Biochemical and biophysical research communications   776   152185 - 152185   2025.6

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    Language:English   Publishing type:Research paper (scientific journal)  

    Abscisic acid (ABA) and strigolactones (SLs) are carotenoid-derived plant hormones with important roles in plant physiological responses. Abscisic acid is involved in drought stress responses and maintaining seed dormancy, whereas SLs are biosynthesized under nutrient-poor conditions and affect plant branching and germination of root parasitic plants. Both ABA and SLs are synthesized from β-carotene via pathways sharing common isomerization and oxidative cleavage reactions. The enzyme D27 catalyzes the isomerization reaction during SL biosynthesis. However, the enzyme involved in the isomerization reaction during ABA biosynthesis remains unclear. Arabidopsis thaliana has two D27 homologs, D27LIKE1 (D27L1) and D27LIKE2 (D27L2). While D27L1 is reported to partially overlap in function with D27, the function of D27L2 remains unknown. Here, we generated single, double, and triple mutants of these genes and examined their physiological functions and involvement in ABA/SL biosynthesis. The results suggested these genes contribute to SL biosynthesis, with functions that complement one another. This would allow each gene to be appropriately regulated in organs requiring SLs. In contrast, our data also indicated that these genes are not involved in ABA biosynthesis. These findings provide important insights into the understanding of carotenoid-derived hormone biosynthesis by demonstrating that D27 homologs are selectively involved in SL, but not ABA, biosynthesis.

    DOI: 10.1016/j.bbrc.2025.152185

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  • 糸状菌由来新規ステロール誘導体であるアミノアシル化ステロールの発見—新たなステロール修飾の発見

    横川 大祐, 久城 哲夫

    化学と生物   62 ( 9 )   421 - 422   2024.9

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:公益社団法人 日本農芸化学会  

    DOI: 10.1271/kagakutoseibutsu.62.421

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    Other Link: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23KJ2002/

  • RNA-dependent synthesis of ergosteryl-3β-O-glycine in Ascomycota expands the diversity of steryl-amino acids Reviewed

    Nathaniel Yakobov, Nassira Mahmoudi, Guillaume Grob, Daisuke Yokokawa, Yusuke Saga, Tetsuo Kushiro, Danielle Worrell, Hervé Roy, Hubert Schaller, Bruno Senger, Laurence Huck, Gisela Riera Gascon, Hubert D. Becker, Frédéric Fischer

    Journal of Biological Chemistry   298 ( 3 )   101657 - 101657   2022.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.jbc.2022.101657

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  • Synthesis of aminoacylated ergosterols: A new lipid component of fungi Reviewed

    Daisuke Yokokawa, Shunsuke Tatematsu, Ryoka Takagi, Yusuke Saga, Hervé Roy, Frédéric Fischer, Hubert D. Becker, Tetsuo Kushiro

    Steroids   169   108823 - 108823   2021.5

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.steroids.2021.108823

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  • Two Triterpene Synthases from <i>Imperata cylindrica</i> Catalyzing the Formation of a Pair of Diastereoisomers through <i>Boat</i> or <i>Chair</i> Cyclization Reviewed

    Shingo Naraki, Mai Kakihara, Sayuri Kato, Yusuke Saga, Kazuto Mannen, Shohei Takase, Akihito Takano, Sayaka Shinpo, Tsutomu Hosouchi, Takahisa Nakane, Hideyuki Suzuki, Tetsuo Kushiro

    ChemBioChem   22 ( 11 )   1992 - 2001   2021.4

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1002/cbic.202100077

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    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/cbic.202100077

  • Plant-Specific Domains and Fragmented Sequences Imply Non-Canonical Functions in Plant Aminoacyl-tRNA Synthetases Reviewed

    Yusuke Saga, Moeka Kawashima, Shiho Sakai, Kaori Yamazaki, Misato Kaneko, Moeka Takahashi, Natsuko Sato, Yohei Toyoda, Shohei Takase, Takeshi Nakano, Naoto Kawakami, Tetsuo Kushiro

    Genes   11 ( 9 )   1056 - 1056   2020.9

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    Aminoacyl-tRNA synthetases (aaRSs) play essential roles in protein translation. In addition, numerous aaRSs (mostly in vertebrates) have also been discovered to possess a range of non-canonical functions. Very few studies have been conducted to elucidate or characterize non-canonical functions of plant aaRSs. A genome-wide search for aaRS genes in Arabidopsis thaliana revealed a total of 59 aaRS genes. Among them, asparaginyl-tRNA synthetase (AsnRS) was found to possess a WHEP domain inserted into the catalytic domain in a plant-specific manner. This insertion was observed only in the cytosolic isoform. In addition, a long stretch of sequence that exhibited weak homology with histidine ammonia lyase (HAL) was found at the N-terminus of histidyl-tRNA synthetase (HisRS). This HAL-like domain has only been seen in plant HisRS, and only in cytosolic isoforms. Additionally, a number of genes lacking minor or major portions of the full-length aaRS sequence were found. These genes encode 14 aaRS fragments that lack key active site sequences and are likely catalytically null. These identified genes that encode plant-specific additional domains or aaRS fragment sequences are candidates for aaRSs possessing non-canonical functions.

    DOI: 10.3390/genes11091056

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  • RNA-dependent sterol aspartylation in fungi Reviewed

    Nathaniel Yakobov, Frédéric Fischer, Nassira Mahmoudi, Yusuke Saga, Christopher D. Grube, Hervé Roy, Bruno Senger, Guillaume Grob, Shunsuke Tatematsu, Daisuke Yokokawa, Isabelle Mouyna, Jean-Paul Latgé, Harushi Nakajima, Tetsuo Kushiro, Hubert D. Becker

    Proceedings of the National Academy of Sciences   117 ( 26 )   14948 - 14957   2020.6

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Proceedings of the National Academy of Sciences  

    Significance

    Bacteria are known to add amino acids (aa) to membrane lipids to resist antimicrobials and escape immune responses. This surface lipid aminoacylation process requires diverting aminoacyl-tRNAs from protein synthesis. While widespread in bacteria, no analogous lipid remodeling system had thus far been evidenced in eukaryotes. We uncovered that most fungi tRNA-dependently add aspartate onto ergosterol (ergosteryl-3β- O - l -aspartate [Erg-Asp]), the major sterol found in fungal membranes. Asp addition is catalyzed by an ergosteryl-3β- O - l -aspartate synthase (ErdS) and its removal by a dedicated hydrolase (ErdH). This pathway is conserved across “higher” fungi, including pathogens. Given the central roles of sterols and derivatives in fungi, we propose that the Erg-Asp homeostasis system might impact membrane remodeling, trafficking, antimicrobial resistance, or pathogenicity.

    DOI: 10.1073/pnas.2003266117

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    Other Link: https://pnas.org/doi/pdf/10.1073/pnas.2003266117

  • Mechanism of Action of Prethioviridamide, an Anticancer Ribosomally Synthesized and Post-Translationally Modified Peptide with a Polythioamide Structure Reviewed

    Shohei Takase, Rumi Kurokawa, Yasumitsu Kondoh, Kaori Honda, Takehiro Suzuki Teppei Kawaha, Haruo Ikeda, Naoshi Dohma, Hiroyuki Osad, Kazuo Shin-y, Tetsuo Kushiro, Minoru Yoshida, Ken Matsumoto

    ACS Chem. Biol.   14   1819 - 1828   2019.7

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    DOI: 10.1021/acschembio.9b00410

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  • Allylic hydroxylation of triterpenoids by a plant cytochrome P450 triggers key chemical transformations that produce a variety of bitter compounds Reviewed International journal

    S. Takase, K. Kera, Y. Nagashima, K. Mannen, T. Hosouchi, S. Shinpo, M. Kawashima, Y. Kotake, H. Yamada, Y. Saga, J. Otaka, H. Araya, M. Kotera, H. Suzuki, T. Kushiro

    J. Biol. Chem.   doi:10.1074/jbc.RA119.009944 ( 49 )   18662 - 18673   2019

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    Cucurbitacins are highly oxygenated triterpenoids characteristic of plants in the family Cucurbitaceae and responsible for the bitter taste of these plants. Fruits of bitter melon (Momordica charantia) contain various cucurbitacins possessing an unusual ether bridge between C5 and C19, not observed in other Cucurbitaceae members. Using a combination of next-generation sequencing and RNA-Seq analysis and gene-to-gene co-expression analysis with the ConfeitoGUIplus software, we identified three P450 genes, CYP81AQ19, CYP88L7, and CYP88L8, expected to be involved in cucurbitacin biosynthesis. CYP81AQ19 co-expression with cucurbitadienol synthase in yeast resulted in the production of cucurbita-5,24-diene-3β,23α-diol. A mild acid treatment of this compound resulted in an isomerization of the C23-OH group to C25-OH with the concomitant migration of a double bond, suggesting that a nonenzymatic transformation may account for the observed C25-OH in the majority of cucurbitacins found in plants. The functional expression of CYP88L7 resulted in the production of hydroxylated C19 as well as C5-C19 ether-bridged products. A plausible mechanism for the formation of the C5-C19 ether bridge involves C7 and C19 hydroxylations, indicating a multifunctional nature of this P450. On the other hand, functional CYP88L8 expression gave a single product, a triterpene diol, indicating a monofunctional P450 catalyzing the C7 hydroxylation. Our findings of the roles of several plant P450s in cucurbitacin biosynthesis reveal that an allylic hydroxylation is a key enzymatic transformation that triggers subsequent processes to produce structurally diverse products.

    DOI: 10.1074/jbc.RA119.009944

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  • Identification of triterpene biosynthetic genes from <I>Momordica charantia</I> using RNA-seq analysis. Reviewed International journal

    Takase S, Kera K, Hirao Y, Hosouchi T, Kotake Y, Nagashima Y, Mannen K, Suzuki H, Kushiro T

    Biosci. Biotechnol. Biochem.   83 ( 2 )   251 - 261   2018

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    Cucurbitaceae plants contain characteristic triterpenoids. Momordica charantia, known as a bitter melon, contains cucurbitacins and multiflorane type triterpenes, which confer bitter tasting and exhibit pharmacological activities. Their carbon skeletons are biosynthesized from 2,3-oxidosqualene by responsible oxidosqualene cyclase (OSC). In order to identify OSCs in M. charantia, RNA-seq analysis was carried out from ten different tissues. The functional analysis of the resulting four OSC genes revealed that they were cucurbitadienol synthase (McCBS), isomultiflorenol synthase (McIMS), β-amyrin synthase (McBAS) and cycloartenol synthase (McCAS), respectively. Their distinct expression patterns based on RPKM values and quantitative RT-PCR suggested how the characteristic triterpenoids were biosynthesized in each tissue. Although cucurbitacins were finally accumulated in fruits, McCBS showed highest expression in leaves indicating that the early step of cucurbitacins biosynthesis takes place in leaves, but not in fruits. Abbreviations: OSC: oxidosqualene cyclase; RPKM: reads perkilobase of exon per million mapped reads.

    DOI: 10.1080/09168451.2018.1530096

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  • FPX is a novel chemical inducer that promotes callus formation and shoot regeneration in plants Reviewed

    Takeshi Nakano, Shota Tanaka, Misato Ohtani, Ayumi Yamagami, Shun Takeno, Naho Hara, Akiko Mori, Ayana Nakano, Sakiko Hirose, Yasuyo Himuro, Masatomo Kobayashi, Tetsuo Kushiro, Taku Demura, Tadao Asami, Hiroyuki Osada, Kazuo Shinozaki

    Plant and Cell Physiology   59 ( 8 )   1555 - 1567   2018

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    DOI: 10.1093/pcp/pcy139

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  • Biosynthesis of helvolic acid and identification of an unusual C-4-demethylation process distinct from sterol biosynthesis Reviewed

    Jian-Ming Lv, Dan Hu, Hao Gao, Tetsuo Kushiro, Takayoshi Awakawa, Guo-Dong Chen, Chuan-Xi Wang, Ikuro Abe, Xin-Sheng Yao

    NATURE COMMUNICATIONS   8   1644   2017.11

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    DOI: 10.1038/s41467-017-01813-9

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  • A quantitative shRNA screen identifies ATP1A1 as a gene that regulates cytotoxicity by aurilide B Reviewed

    Shohei Takase, Rumi Kurokawa, Daisuke Arai, Kind Kanemoto Kanto, Tatsufumi Okino, Yoichi Nakao, Tetsuo Kushiro, Minoru Yoshida, Ken Matsumoto

    SCIENTIFIC REPORTS   7   2002   2017.5

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    DOI: 10.1038/s41598-017-02016-4

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  • Identification of Serratane Synthase Gene from the Fern Lycopodium clavatum Reviewed

    Yusuke Saga, Takeshi Araki, Hiroshi Araya, Kazuki Saito, Mami Yamazaki, Hideyuki Suzuki, Tetsuo Kushiro

    ORGANIC LETTERS   19 ( 3 )   496 - 499   2017.2

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    DOI: 10.1021/acs.orglett.6b03659

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  • A draft genome of the brown alga, Cladosiphon okamuranus, S-strain: a platformfor future studies of 'mozuku' biology Reviewed

    Koki Nishitsuji, Asuka Arimoto, Kenji Iwai, Yusuke Sudo, Kanako Hisata, Manabu Fujie, Nana Arakaki, Tetsuo Kushiro, Teruko Konishi, Chuya Shinzato, Noriyuki Satoh, Eiichi Shoguchi

    DNA RESEARCH   23 ( 6 )   561 - 570   2016.12

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    DOI: 10.1093/dnares/dsw039

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  • Highly Sprouting-Tolerant Wheat Grain Exhibits Extreme Dormancy and Cold Imbibition-Resistant Accumulation of Abscisic Acid Reviewed

    Yu-ichi Kashiwakura, Daisuke Kobayashi, Yusuke Jikumaru, Yumiko Takebayashi, Eiji Nambara, Mitsunori Seo, Yuji Kamiya, Tetsuo Kushiro, Naoto Kawakami

    PLANT AND CELL PHYSIOLOGY   57 ( 4 )   715 - 732   2016.4

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    DOI: 10.1093/pcp/pcw051

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  • Onocerin Biosynthesis Requires Two Highly Dedicated Triterpene Cyclases in a Fern Lycopodium clavatum Reviewed

    Takeshi Araki, Yusuke Saga, Momo Marugami, Junnosuke Otaka, Hiroshi Araya, Kazuki Saito, Mami Yamazaki, Hideyuki Suzuki, Tetsuo Kushiro

    CHEMBIOCHEM   17 ( 4 )   288 - 290   2016.2

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    DOI: 10.1002/cbic.201500663

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  • Control of the 1,2-rearrangement process by oxidosqualene cyclases during triterpene biosynthesis Reviewed

    Shohei Takase, Yusuke Saga, Nozomi Kurihara, Shingo Naraki, Kenta Kuze, Genki Nakata, Takeshi Araki, Tetsuo Kushiro

    ORGANIC & BIOMOLECULAR CHEMISTRY   13 ( 26 )   7331 - 7336   2015

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    DOI: 10.1039/c5ob00714c

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  • Terretonin Biosynthesis Requires Methylation as Essential Step for Cyclization Reviewed

    Yudai Matsuda, Takayoshi Awakawa, Takayuki Itoh, Toshiyuki Wakimoto, Tetsuo Kushiro, Isao Fujii, Yutaka Ebizuka, Ikuro Abe

    CHEMBIOCHEM   13 ( 12 )   1738 - 1741   2012.8

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    DOI: 10.1002/cbic.201200369

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  • Identification of a Key Prenyltransferase Involved in Biosynthesis of the Most Abundant Fungal Meroterpenoids Derived from 3,5-Dimethylorsellinic Acid. Reviewed

    Takayuki Itoh, Kinya Tokunaga, Edayileveettil K. Radhakrishnan, Isao Fujii, Ikuro Abe, Yutaka Ebizuka, Tetsuo Kushiro

    CHEMBIOCHEM   13 ( 8 )   1132 - 1135   2012.5

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    DOI: 10.1002/cbic.201200124

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  • Benzophenone synthase from Garcinia mangostana L. pericarps

    Natsajee Nualkaew, Hiroyuki Morita, Yoshihiko Shimokawa, Keishi Kinjo, Tetsuo Kushiro, Wanchai De-Eknamkul, Yutaka Ebizuka, Ikuro Abe

    PHYTOCHEMISTRY   77   60 - 69   2012.5

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    DOI: 10.1016/j.phytochem.2012.02.002

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  • ABA 9 &apos;-hydroxylation is catalyzed by CYP707A in Arabidopsis Reviewed

    Masanori Okamoto, Tetsuo Kushiro, Yusuke Jikumaru, Suzanne R. Abrams, Yuji Kamiya, Motoaki Seki, Eiji Nambara

    PHYTOCHEMISTRY   72 ( 8 )   717 - 722   2011.6

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    DOI: 10.1016/j.phytochem.2011.02.004

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  • Linking Genotype and Phenotype of Saccharomyces cerevisiae Strains Reveals Metabolic Engineering Targets and Leads to Triterpene Hyper-Producers Reviewed

    Karina M. Madsen, Gupta D. B. R. K. Udatha, Saori Semba, Jose M. Otero, Peter Koetter, Jens Nielsen, Yutaka Ebizuka, Tetsuo Kushiro, Gianni Panagiotou

    PLOS ONE   6 ( 3 )   e14763   2011.3

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    DOI: 10.1371/journal.pone.0014763

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  • Reconstitution of a fungal meroterpenoid biosynthesis reveals the involvement of a novel family of terpene cyclases Reviewed

    Takayuki Itoh, Kinya Tokunaga, Yudai Matsuda, Isao Fujii, Ikuro Abe, Yutaka Ebizuka, Tetsuo Kushiro

    NATURE CHEMISTRY   2 ( 10 )   858 - 864   2010.10

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    DOI: 10.1038/NCHEM.764

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  • Protostadienol synthase from Aspergillus fumigatus: Functional conversion into lanosterol synthase Reviewed

    Miki Kimura, Tetsuo Kushiro, Masaaki Shibuya, Yutaka Ebizuka, Ikuro Abe

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   391 ( 1 )   899 - 902   2010.1

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    DOI: 10.1016/j.bbrc.2009.11.160

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  • Biosynthesis of Steroidal Antibiotic Fusidanes: Functional Analysis of Oxidosqualene Cyclase and Subsequent Tailoring Enzymes from Aspergillus fumigatus Reviewed

    Hisashi Mitsuguchi, Yasuyo Seshime, Isao Fujii, Masaaki Shibuya, Yutaka Ebizuka, Tetsuo Kushiro

    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY   131 ( 18 )   6402 - 6411   2009.5

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    DOI: 10.1021/ja8095976

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  • Defining the sequence region responsible for Baeyer-Villiger oxidation in CYP85A3 involved in plant hormone brassinolide biosynthesis Reviewed

    T. Kushiro, H. Mitsuguchi, T. Nomura, S. Yamaguchi, Y. Kamiya, Y. Ebizuka

    16TH INTERNATIONAL CONFERENCE ON CYTOCHROME P450, PROCEEDINGS   45 - +   2009

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  • Biosynthesis of Baccharis Oxide, a Triterpene with a 3,10-Oxide Bridge in the A-Ring Reviewed

    Masaaki Shibuya, Aya Sagara, Ai Saitoh, Tetsuo Kushiro, Yutaka Ebizuka

    ORGANIC LETTERS   10 ( 21 )   5071 - 5074   2008.11

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    DOI: 10.1021/ol802072y

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  • Phytochrome- and gibberellin-mediated regulation of abscisic acid metabolism during germination of photoblastic lettuce seeds Reviewed

    Yoshiaki Sawada, Miki Aoki, Kentaro Nakaminami, Wataru Mitsuhashi, Kiyoshi Tatematsu, Tetsuo Kushiro, Tomokazu Koshiba, Yuji Kamiya, Yasunori Inoue, Eiji Nambara, Tomonobu Toyomasu

    PLANT PHYSIOLOGY   146 ( 3 )   1386 - 1396   2008.3

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    DOI: 10.1104/pp.107.115162

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  • Ethylene promotes submergence-induced expression of OsABA8ox1, a gene that encodes ABA 8'-hydroxylase in rice. Reviewed

    Hiroaki Saika, Masanori Okamoto, Kentaro Miyoshi, Tetsuo Kushiro, Shoko Shinoda, Yusuke Jikumaru, Masaru Fujimoto, Taku Arikawa, Hirokazu Takahashi, Miho Ando, Shin-Ichi Arimura, Akio Miyao, Hirohiko Hirochika, Yuji Kamiya, Nobuhiro Tsutsumi, Eiji Nambara, Mikio Nakazono

    Plant & cell physiology   48 ( 2 )   287 - 98   2007.2

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    A rapid decrease of the plant hormone ABA under submergence is thought to be a prerequisite for the enhanced elongation of submerged shoots of rice (Oryza sativa L.). Here, we report that the level of phaseic acid (PA), an oxidized form of ABA, increased with decreasing ABA level during submergence. The oxidation of ABA to PA is catalyzed by ABA 8'-hydroxylase, which is possibly encoded by three genes (OsABA8ox1, -2 and -3) in rice. The ABA 8'-hydroxylase activity was confirmed in microsomes from yeast expressing OsABA8ox1. OsABA8ox1-green fluorescent protein (GFP) fusion protein in onion cells was localized to the endoplasmic reticulum. The mRNA level of OsABA8ox1, but not the mRNA levels of other OsABA8ox genes, increased dramatically within 1 h after submergence. On the other hand, the mRNA levels of genes involved in ABA biosynthesis (OsZEP and OsNCEDs) decreased after 1-2 h of submergence. Treatment of aerobic seedlings with ethylene and its precursor, 1-aminocyclopropane-1-carboxylate (ACC), rapidly induced the expression of OsABA8ox1, but the ethylene treatment did not strongly affect the expression of ABA biosynthetic genes. Moreover, pre-treatment with 1-methylcyclopropene (1-MCP), a potent inhibitor of ethylene action, partially suppressed induction of OsABA8ox1 expression under submergence. The ABA level was found to be negatively correlated with OsABA8ox1 expression under ACC or 1-MCP treatment. Together, these results indicate that the rapid decrease in ABA levels in submerged rice shoots is controlled partly by ethylene-induced expression of OsABA8ox1 and partly by ethylene-independent suppression of genes involved in the biosynthesis of ABA.

    DOI: 10.1093/pcp/pcm003

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  • 植物トリテルペン生合成の生物有機化学 Reviewed

    久城哲夫, 海老塚豊

    蛋白質核酸酵素 10月号増刊号ケミカルバイオロジー   52 ( 10月号増刊 )   1730 - 1735   2007

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    「植物トリテルペン生合成の生物有機化学」1730-1735

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  • Dwarfism and cytochrome P450-mediated C-6 oxidation of plant steroid hormones Reviewed

    G. Bishop, T. Nomura, T. Yokota, T. Montoya, J. Castle, K. Harrison, T. Kushiro, Y. Kamiya, S. Yamaguchi, S. Bancos, A. -M. Szatmari, M. Szekeres

    BIOCHEMICAL SOCIETY TRANSACTIONS   34   1199 - 1201   2006.12

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    DOI: 10.1042/BST0341199

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  • Stereochemical course in water addition during LUP1-catalyzed triterpene cyclization Reviewed

    Tetsuo Kushiro, Masaki Hoshino, Takehiko Tsutsumi, Ken-ichi Kawai, Motoo Shiro, Masaaki Shibuya, Yutaka Ebizuka

    ORGANIC LETTERS   8 ( 24 )   5589 - 5592   2006.11

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    DOI: 10.1021/ol062310d

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  • Dammarenediol-II synthase, the first dedicated enzyme for ginsenoside biosynthesis, in Panax ginseng Reviewed

    Pimpimon Tansakul, Masaaki Shibuya, Tetsuo Kushiro, Yutaka Ebizuka

    FEBS LETTERS   580 ( 22 )   5143 - 5149   2006.10

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    DOI: 10.1016/j.febslet.2006.08.044

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  • P-392 BIOSYNTHETIC PATHWAY OF PHYTOSTEROLS VIA LANOSTEROL

    Ohyama Kiyoshi, Suzuki Masashi, Xiang Ting, Seki Hikaru, Kikuchi Jun, Saito Kazuki, Muranaka Toshiya, Katsube Yuji, Kushiro Tetsuo, Shibuya Masaaki, Ebizuka Yutaka

    International Symposium on the Chemistry of Natural Products   2006   "P - 392"   2006.7

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    Language:English   Publisher:Symposium on the chemistry of natural products  

    DOI: 10.24496/intnaturalprod.2006.0__P-392_

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  • A plant growth retardant, uniconazole, is a potent inhibitor of ABA catabolism in Arabidopsis Reviewed

    Shigeki Saito, Masanori Okamoto, Shoko Shinoda, Tetsuo Kushiro, Tomokazu Koshiba, Yuji Kamiya, Nobuhiro Hirai, Yasushi Todoroki, Kanzo Sakata, Eiji Nambara, Masaharu Mizutani

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   70 ( 7 )   1731 - 1739   2006.7

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    DOI: 10.1271/bbb.60077

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  • CYP707A1 and CYP707A2, which encode abscisic acid 8 &apos;-hydroxylases, are indispensable for proper control of seed dormancy and germination in Arabidopsis Reviewed

    M Okamoto, A Kuwahara, M Seo, T Kushiro, T Asami, N Hirai, Y Kamiya, T Koshiba, E Nambara

    PLANT PHYSIOLOGY   141 ( 1 )   97 - 107   2006.5

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    DOI: 10.1104/pp.106.079475

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  • Lanosterol synthase in dicotyledonous plants Reviewed

    Masashi Suzuki, Ting Xiang, Kiyoshi Ohyama, Hikaru Seki, Kazuki Saito, Toshiya Muranaka, Hiroaki Hayashi, Yuji Katsube, Tetsuo Kushiro, Masaaki Shibuya, Yutaka Ebizuka

    PLANT AND CELL PHYSIOLOGY   47 ( 5 )   565 - 571   2006.5

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    DOI: 10.1093/pcp/pcj031

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  • CYP707A3, a major ABA 8 '-hydroxylase involved in dehydration and rehydration response in Arabidopsis thaliana Reviewed

    T Umezawa, M Okamoto, T Kushiro, E Nambara, Y Oono, M Seki, M Kobayashi, T Koshiba, Y Kamiya, K Shinozaki

    PLANT JOURNAL   46 ( 2 )   171 - 182   2006.4

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    DOI: 10.1111/j.1365-313X.2006.02683.X

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  • Identification of beta-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay Reviewed

    M Shibuya, M Hoshino, Y Katsube, H Hayashi, T Kushiro, Y Ebizuka

    FEBS JOURNAL   273 ( 5 )   948 - 959   2006.3

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    DOI: 10.1111/j.1742-4658.2006.05120.x

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  • Field studies on the regulation of abscisic acid content and germinability during grain development of barley: molecular and chemical analysis of pre-harvest sprouting. Reviewed International journal

    Makiko Chono, Ichiro Honda, Shoko Shinoda, Tetsuo Kushiro, Yuji Kamiya, Eiji Nambara, Naoto Kawakami, Shigenobu Kaneko, Yoshiaki Watanabe

    Journal of experimental botany   57 ( 10 )   2421 - 34   2006

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    To investigate whether the regulation of abscisic acid (ABA) content was related to germinability during grain development, two cDNAs for 9-cis-epoxycarotenoid dioxygenase (HvNCED1 and HvNCED2) and one cDNA for ABA 8'-hydroxylase (HvCYP707A1), which are enzymes thought to catalyse key regulatory steps in ABA biosynthesis and catabolism, respectively, were cloned from barley (Hordeum vulgare L.). Expression and ABA-quantification analysis in embryo revealed that HvNCED2 is responsible for a significant increase in ABA levels during the early to middle stages of grain development, and HvCYP707A1 is responsible for a rapid decrease in ABA level thereafter. The change in the embryonic ABA content of imbibing grains following dormancy release is likely to reflect changes in the expression patterns of HvNCEDs and HvCYP707A1. A major change between dormant and after-ripened grains occurred in HvCYP707A1; the increased expression of HvCYP707A1 in response to imbibition, followed by a rapid ABA decrease and a high germination percentage, was observed in the after-ripened grains, but not in the dormant grains. Under field conditions, HvNCED2 showed the same expression level and pattern during grain development in 2002, 2003, and 2004, indicating that HvNCED2 expression is regulated in a growth-dependent manner in the grains. By contrast, HvNCED1 and HvCYP707A1 showed a different expression pattern in each year, indicating that the expression of these genes is affected by environmental conditions during grain development. The varied expression levels of these genes during grain development and imbibition, which would have effects on the activity of ABA biosynthesis and catabolism, might be reflected, in part, in the germinability in field-grown barley.

    DOI: 10.1093/jxb/erj215

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  • The last reaction producing brassinolide is catalyzed by cytochrome P-450s, CYP85A3 in tomato and CYP85A2 in Arabidopsis Reviewed

    T Nomura, T Kushiro, T Yokota, Y Kamiya, GJ Bishop, S Yamaguchi

    JOURNAL OF BIOLOGICAL CHEMISTRY   280 ( 18 )   17873 - 17879   2005.5

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    DOI: 10.1074/jbc.M414592200

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  • The Arabidopsis cytochrome P450CYP707A encodes ABA 8 '-hydroxylases: key enzymes in ABA catabolism Reviewed

    T Kushiro, M Okamoto, K Nakabayashi, K Yamagishi, S Kitamura, T Asami, N Hirai, T Koshiba, Y Kamiya, E Nambara

    EMBO JOURNAL   23 ( 7 )   1647 - 1656   2004.4

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    DOI: 10.1038/sj.emboj.7600121

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  • The key to signalling Reviewed

    T Kushiro, E Nambara, P McCourt

    NATURE   422 ( 6928 )   122 - 122   2003.3

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    DOI: 10.1038/422122a

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  • Functional genomics approach to the study of triterpene biosynthesis Reviewed

    Y Ebizuka, Y Katsube, T Tsutsumi, T Kushiro, M Shibuya

    PURE AND APPLIED CHEMISTRY   75 ( 2-3 )   369 - 374   2003.2

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    DOI: 10.1351/pac200375020369

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  • Trbp 111 selectively binds a noncovalently assembled tRNA-like structure Reviewed

    T Kushiro, P Schimmel

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   99 ( 26 )   16631 - 16635   2002.12

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    DOI: 10.1073/pnas.262667999

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  • Molecular cloning and characterization of isomultiflorenol synthase, a new triterpene synthase from Luffa cylindrica, involved in biosynthesis of bryonolic acid Reviewed

    H Hayashi, P Huang, K Inoue, N Hiraoka, Y Ikeshiro, K Yazaki, S Tanaka, T Kushiro, M Shibuya, Y Ebizuka

    EUROPEAN JOURNAL OF BIOCHEMISTRY   268 ( 23 )   6311 - 6317   2001.12

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    DOI: 10.1046/j.0014-2956.2001.02588.x

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  • Cloning and characterization of a cDNA encoding beta-amyrin synthase involved in glycyrrhizin and soyasaponin biosyntheses in licorice Reviewed

    H Hayashi, PY Huang, A Kirakosyan, K Inoue, N Hiraoka, Y Ikeshiro, T Kushiro, M Shibuya, Y Ebizuka

    BIOLOGICAL & PHARMACEUTICAL BULLETIN   24 ( 8 )   912 - 916   2001.8

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    DOI: 10.1248/bpb.24.912

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  • A novel multifunctional triterpene synthase from Arabidopsis thaliana Reviewed

    T Kushiro, M Shibuya, K Masuda, Y Ebizuka

    TETRAHEDRON LETTERS   41 ( 40 )   7705 - 7710   2000.9

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    DOI: 10.1016/S0040-4039(00)01347-2

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  • Mutational studies on triterpene syntheses: Engineering lupeol synthase into beta-amyrin synthase Reviewed

    T Kushiro, M Shibuya, K Masuda, Y Ebizuka

    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY   122 ( 29 )   6816 - 6824   2000.7

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    DOI: 10.1021/ja0010709

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  • Molecular cloning and functional expression of triterpene synthases from pea (Pisum sativum) - New alpha-amyrin-producing enzyme is a multifunctional triterpene synthase Reviewed

    M Morita, M Shibuya, T Kushiro, K Masuda, Y Ebizuka

    EUROPEAN JOURNAL OF BIOCHEMISTRY   267 ( 12 )   3453 - 3460   2000.6

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    DOI: 10.1046/j.1432-1327.2000.01357.x

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  • Molecular cloning and characterization of a cDNA for Glycyrrhiza glabra cycloartenol synthase Reviewed

    H Hayashi, N Hiraoka, Y Ikeshiro, T Kushiro, M Morita, M Shibuya, Y Ebizuka

    BIOLOGICAL & PHARMACEUTICAL BULLETIN   23 ( 2 )   231 - 234   2000.2

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    DOI: 10.1248/bpb.23.231

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  • Biosynthesis of 20-hydroxyecdysone in Ajuga hairy roots: Fate of 6 alpha- and 6 alpha-hydrogens of lathosterol Reviewed

    K Ohyama, T Kushiro, K Nakamura, Y Fujimoto

    BIOORGANIC & MEDICINAL CHEMISTRY   7 ( 12 )   2925 - 2930   1999.12

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    DOI: 10.1016/S0968-0896(99)00243-6

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  • Two branches of the lupeol synthase gene in the molecular evolution of plant oxidosqualene cyclases Reviewed

    M Shibuya, H Zhang, A Endo, K Shishikura, T Kushiro, Y Ebizuka

    EUROPEAN JOURNAL OF BIOCHEMISTRY   266 ( 1 )   302 - 307   1999.11

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    DOI: 10.1046/j.1432-1327.1999.00875.x

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  • Cryptic regiospecificity in deprotonation step of triterpene biosynthesis catalyzed by new members of lupeol synthase Reviewed

    T Kushiro, M Shibuya, Y Ebizuka

    TETRAHEDRON LETTERS   40 ( 30 )   5553 - 5556   1999.7

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    DOI: 10.1016/S0040-4039(99)01035-7

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  • Chimeric triterpene synthase. A possible model for multifunctional triterpene synthase Reviewed

    T Kushiro, M Shibuya, Y Ebizuka

    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY   121 ( 6 )   1208 - 1216   1999.2

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    DOI: 10.1021/ja983012h

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  • beta-Amyrin synthase - Cloning of oxidosqualene cyclase that catalyzes the formation of the most popular triterpene among higher plants Reviewed

    T Kushiro, M Shibuya, Y Ebizuka

    EUROPEAN JOURNAL OF BIOCHEMISTRY   256 ( 1 )   238 - 244   1998.8

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    DOI: 10.1046/j.1432-1327.1998.2560238.x

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  • Biosynthesis of 20-hydroxyecdysone in Ajuga hairy roots: Hydrogen migration from C-6 to C-5 during cis-A/B ring formation Reviewed

    Y Fujimoto, T Kushiro, K Nakamura

    TETRAHEDRON LETTERS   38 ( 15 )   2697 - 2700   1997.4

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    DOI: 10.1016/S0040-4039(97)00432-2

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  • In vitro conversion of 2,3-oxidosqualene into dammarenediol by Panax ginseng microsomes Reviewed

    T Kushiro, Y Ohno, M Shibuya, Y Ebizuka

    BIOLOGICAL & PHARMACEUTICAL BULLETIN   20 ( 3 )   292 - 294   1997.3

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  • Biosynthesis of 24 beta-alkyl-Delta(25)-sterols in hairy roots of Ajuga reptans var atropurpurea Reviewed

    T Yagi, M Morisaki, T Kushiro, H Yoshida, Y Fujimoto

    PHYTOCHEMISTRY   41 ( 4 )   1057 - 1064   1996.3

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    DOI: 10.1016/0031-9422(95)00766-0

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  • 3-BETA-HYDROXY-5-BETA-CHOLEST-7-EN-6-ONE AS AN INTERMEDIATE OF 20-HYDROXYECDYSONE BIOSYNTHESIS IN A HAIRY ROOT CULTURE OF AJUGA-REPTANS VAR ATROPURPUREA Reviewed

    M NAGAKARI, T KUSHIRO, T YAGI, N TANAKA, T MATSUMOTO, K KAKINUMA, Y FUJIMOTO

    JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS   1994 ( 15 )   1761 - 1762   1994.8

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    DOI: 10.1039/c39940001761

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  • INCORPORATION OF ACETATE AND CHOLESTEROL INTO 20-HYDROXYECDYSONE BY HAIRY ROOT CLONE OF AJUGA-REPTANS VAR ATROPURPUREA Reviewed

    M NAGAKARI, T KUSHIRO, T MATSUMOTO, N TANAKA, K KAKINUMA, Y FUJIMOTO

    PHYTOCHEMISTRY   36 ( 4 )   907 - 910   1994.7

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    DOI: 10.1016/S0031-9422(00)90461-8

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Books

  • Reconstitution of a secondary metabolite biosynthetic pathway in a heterologous fungal host.

    T. Itoh, T. Kushiro, I. Fujii( Role: Joint author)

    Springler  2012 

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  • Triterpenes

    T. Kushiro, Y. Ebizuka( Role: Joint author)

    Elsevier: Oxford  2010 

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    Triterpenes in Comprehensive Natural Products II 673-708

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  • 生薬学へのいざない? 生薬学は今日の医療にどう役立つのか?

    伊藤美千穂, 池田剛, 久城哲夫( Role: Joint author)

    京都廣川書店  2009.3 

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  • アブシジン酸不活性化による休眠解除

    久城哲夫, 岡本昌憲( Role: Joint author)

    文一総合出版  2009.3 

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    アブシジン酸不活性化による休眠解除 279-286

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  • Enzymatic synthesis of cyclic triterpenes

    T. Kushiro, I. Abe( Role: Joint author)

    Nova Publishers  2009 

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  • ABAの代謝に関わるシトクロムP450モノオキシゲナーゼ

    久城哲夫, 南原英司( Role: Joint author)

    秀潤社  2004 

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    Short Topics ABAの代謝に関わるシトクロムP450モノオキシゲナーゼ 133-136

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  • Molecular Cloning of Oxidosqualene Cyclase cDNA from <I>Panax ginseng</I>: The Isogene that Encodes β-Amyrin Synthase.

    T. Kushiro, M.Shibuya, Y.Ebizuka( Role: Joint author)

    "Elsevier Science, Amsterdam"  1998 

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    Molecular Cloning of Oxidosqualene Cyclase cDNA from Panax ginseng: The Isogene that Encodes β-Amyrin Synthase. 421-427

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MISC

  • ラノステロールを経由する植物ステロール新規生合成経路

    大山清, 鈴木優志, TING Xiang, 關光, 菊地淳, 久城哲夫, 渋谷雅明, 海老塚豊, 斉藤和季, 村中俊哉

    天然有機化合物討論会講演要旨集   49th   37 - 42   2007.8

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    J-GLOBAL

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  • 57. Functional analysis of CYP85A family involved in brassinolide biosynthesis

    Mitsuguchi Hisashi, Nomura Takahito, Kamiya Yuji, Yamaguchi Shinjiro, Ebizuka Yutaka, Kushiro Tetsuo

    The Janapese Society for Chemical Regulation of Plants, Abstract   42   70 - 70   2007

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    Brassinosteroids (BRs) are plant steroid hormones that are essential for normal plant growth and development. Cytochrome P450 monooxygenases play crucial roles in BR biosynthesis, among them, CYP85A families catalyze the last step in the BRs biosynthesis and yield brassinolide (BL) which is the most bioactive BR. It has been shown that CYP85A1 from Arabidopsis thaliana and tomato convert 6-deoxocastasterone (6-deoxoCS) to castasterone (CS), while A. thaliana CYP85A2 and tomato CYP85A3 convert 6-deoxoCS to BL. Although tomato CYP85A1 and CYP85A3 show 85% sequence similarity at the amino acid level, reactions catalyzed by them are different. In order to identify amino acid residues which are crucial for Baeyer-Villiger oxidation involved in BL biosynthesis, we have carried out chimeric studies between tomato CYP85A1 and A3.

    DOI: 10.18978/jscrpanb.42.0_70

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  • Physiological roles of ABA 8 '-hydroxylase family, key enzymes in ABA catabolism during seed development through to postgermination growth in Arabidopsis thaliana

    M Okamoto, A Kuwahara, T Kushiro, Y Kamiya, T Koshiba, E Nambara

    PLANT AND CELL PHYSIOLOGY   47   S147 - S147   2006

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  • Uniconazole-P is a potent inhibitor for ABA catabolism

    S Saito, M Okamoto, S Shinoda, T Kushiro, Y Kamiya, E Nambara, N Hirai, K Sakata, M Mizutani

    PLANT AND CELL PHYSIOLOGY   46   S215 - S215   2005

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  • Physiological role of ABA 8 '-hydroxylase family, key enzymes in ABA catabolism in Arabidopsis thaliana

    M Okamoto, T Kushiro, T Asami, T Koshiba, Y Kamiya, E Nambara

    PLANT AND CELL PHYSIOLOGY   46   S50 - S50   2005

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  • Identification of a cytochrorne P450 nionooxygenase that produces brassinolide, the most active brassinosteroid

    T Nomura, T Kushiro, T Yokota, Y Kamiya, G Bishop, S Yamaguchi

    PLANT AND CELL PHYSIOLOGY   46   S49 - S49   2005

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  • Identification of Arabidopsis cytochrome P450 gene responsible for abscisic acid catabolism

    T Kushiro, M Okamoto, K Nakabayashi, N Hirai, T Asami, T Koshiba, Y Kamiya, E Nambara

    PLANT AND CELL PHYSIOLOGY   45   S75 - S75   2004

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Presentations

  • Functional analysis of plant double-length tyrosyl-tRNA synthetase from <I>Arabidopsis thaliana</I>. International conference

    T. Kushiro, Y. Toyoda, Y. Saga, S. Tatematsu, M. Wada, K. Endo, K. Ito

    27th tRNA conference  2018.9 

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    Venue:Strasbourg, France  

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  • Arabidopsis histidyl-tRNA synthetase with an appended domain bestowed anthocyanin and lignin production upon overexpression in plants. International conference

    M. Kawashima, Y. Saga, S. Sakai, K. Yamazaki, N. Kawakami, M. Wada, K. Endo, K. Ito, T. Nakano, T. Kushiro

    IUBMB Focused Meeting on the Aminoacyl-tRNA Synthetases.  2017.11 

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    Venue:Clearwater, Florida, USA  

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  • 植物由来アミノアシルtRNA合成酵素の二次機能の探索

    川島萌華, 佐賀裕亮, 坂井志帆, 中野雄司, 川上直人, 久城哲夫

    第39回日本分子生物学会年会  2016.12 

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    Venue:横浜、神奈川  

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  • シロイヌナズナ由来二量体型チロシルtRNA合成酵素の機能解析

    豊田陽平, 川島萌華, 佐賀裕亮, 和田美紀, 伊藤耕一, 久城哲夫

    第39回日本分子生物学会年会  2016.11 

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    Venue:横浜、神奈川  

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  • Studies on plant and fungal tRNA synthetases in search for non-canonical functions. International conference

    T. Kushiro, Y. Saga, M. Kawashima, Y. Toyoda, S. Sakai, N. Kawakami, H. Nakajima, T. Nakano, M. Wada, K. Endo, K. Ito

    26th tRNA Conference  2016.9 

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    Venue:Jeju, Korea  

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  • シロイヌナズナ由来二量体型チロシルtRNA合成酵素の機能解析

    豊田陽平, 川島萌華, 佐賀祐亮, 和田美紀, 伊藤耕一, 久城哲夫

    日本農芸化学会2016年度大会  2016.3 

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    Venue:札幌、北海道  

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  • シロイヌナズナ由来カロテノイド異性化酵素の探索

    川名誠, 相馬史幸, 小竹英一, 都築和香子, 長尾昭彦, 川上直人, 久城哲夫

    日本農芸化学会2016年度大会  2016.3 

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    Venue:札幌、北海道  

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  • ヒカゲノカズラ由来オノセリンおよびセラタン型トリテルペンの生合成遺伝子の同定

    佐賀祐亮, 荒木健志, 丸上萌々, 大高潤之介, 荒谷博, 斉藤和季, 山崎真己, 鈴木秀幸, 久城哲夫

    日本農芸化学会2016年度大会  2016.3 

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    Venue:札幌、北海道  

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  • 植物由来アミノアシルtRNA合成酵素の二次機能の探索

    川島萌華, 佐賀祐亮, 伊藤耕一, 川上直人, 久城哲夫

    第57回日本植物生理学会年会  2016.3 

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    Venue:盛岡、岩手  

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  • 植物由来アミノアシルtRNA合成酵素の二次機能の探索

    川島萌華, 佐賀祐亮, 川上直人, 久城哲夫

    第38回日本分子生物学会年会  2015.12 

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    Venue:神戸、兵庫  

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  • Characterization of plant and fungal aaRS sequences in search for non-canonical functions. International conference

    Y. Saga, M. Kawashima, N. Kawakami, H. Nakajima, T. Kushiro

    10th International Symposium on Aminoacyl-tRNA Synthetases.  2015.10 

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    Venue:Barcelona, Spain  

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  • ヒカゲノカズラ由来オノセリン生合成に関与する環化酵素遺伝子の同定

    佐賀祐亮, 荒木健志, 丸上萌々, 荒谷博, 斉藤和季, 山崎真己, 鈴木秀幸, 久城哲夫

    第33回日本植物細胞分子生物学会(東京)大会  2015.8 

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    Venue:東京  

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  • ヒカゲノカズラ由来新規トリテルペン合成酵素の遺伝子クローニング

    荒木健志, 荒谷博, 久城哲夫

    日本農芸化学会2014年度大会  2014.3 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京  

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  • Reconstituted biosynthesis of meroterpenoid in fungi International conference

    Tetsuo Kushiro

    Pacifichem 2010  2010.12 

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    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:Honolulu, Hawaii, USA  

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  • 薬用成分の生合成研究

    久城哲夫

    薬用植物フォーラム2009(つくば国際会議場)  2009.7 

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    Language:Japanese   Presentation type:Oral presentation (general)  

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  • トリテルペン生合成の生物有機化学

    久城哲夫

    日本薬学会第129年会一般シンポジウム(京都国際会館)  2009.3 

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    Language:Japanese   Presentation type:Oral presentation (general)  

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  • Biosynthesis of fungal meroterpenoids: a rich source of promising drug candidates.

    T. Kushiro, T. Itoh, Y. Seshime, I. Fujii, Y. Ebizuka

    The eighth NRCT-JSPS Joint Seminar  2009.2  "Chulalongkorn University, Thailand"

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    Language:English   Presentation type:Oral presentation (general)  

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  • Functional analysis of fungal meroterpenoid gene cluster

    T. Kushiro, T. Itoh, Y. Seshime, I. Fujii, Y. Ebizuka

    7th US-Japan seminar on the biosynthesis of natural products  2008.6  "La Jolla, California, USA"

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    Language:English   Presentation type:Oral presentation (general)  

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  • 植物由来トリテルペノイド生合成酵素を活用した有用物質生産

    久城哲夫

    日本農芸化学会2007年度大会シンポジウム  2007.3  東京農業大学

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    Language:Japanese   Presentation type:Oral presentation (general)  

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Research Projects

  • Physiological role of novel sterol-amino acid derivatives in fungal reproduction.

    Grant number:24K08735  2024.4 - 2027.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

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  • Functional analysis of novel aminoacyl-sterol derivatives in fungi

    Grant number:21K05406  2021.4 - 2024.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant amount:\4290000 ( Direct Cost: \3300000 、 Indirect Cost:\990000 )

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  • 環境応答機能の解明 に基づく高度環境適応植物デザイン研究基盤の確立

    2014.4 - 2019.3

    私立大学戦略的研究基盤形成支援事業 

    川上直人

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    Grant type:Competitive

    Grant amount:\11300000 ( Direct Cost: \11300000 )

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  • 機能性成分の生合成遺伝子マーカーの探索

    2012.4 - 2016.3

    沖縄県水産海洋技術センター 

    久城哲夫

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\4220000 ( Direct Cost: \4220000 )

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  • Enzyme engineering for natural product biosynthesis

    Grant number:23241068  2011.4 - 2015.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

    ABE IKURO, WAKIMOTO Toshiyuki, AWAKAWA Takayoshi, MATSUDA Yudai, MORITA Hiroyuki, SUGIO Shigetoshi, NOGUCHI Hiroshi, KUSHIRO Testuo

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    Grant amount:\49400000 ( Direct Cost: \38000000 、 Indirect Cost:\11400000 )

    Several enzymes involved in the biosynthesis of natural products, such as plant polyketide synthase and CoA-ligase, exhibit remarkable catalytic versatility and substrate promiscuity. This makes the enzymes an excellent platform for enzyme engineering and further production of structurally divergent molecules. In this study, on the basis of the X-ray crystal structures of the enzymes, we succeeded in production of chemically and structurally divergent unnatural natural products, by structure-based enzyme engineering and precursor directed biosynthesis. In addition, we succeeded in elucidation of biosynthetic machinery of complex natural products including calyculin, teleocidin, and antimycin.

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  • ステロイド類生合成修飾酵素を活用した有用物質生産系の構築

    2011.4 - 2014.3

    新学術領域研究(研究領域提案型•公募研究) 

    久城哲夫

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\7020000 ( Direct Cost: \5400000 、 Indirect Cost:\1620000 )

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  • トランスポーター様新規テルペン環化酵素の機能解析

    2011.4 - 2013.3

    基盤研究(C) 

    久城哲夫

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\4550000 ( Direct Cost: \3500000 、 Indirect Cost:\1050000 )

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  • Generating molecular diversity by the wide range of polyketide synthases

    Grant number:22241054  2010 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

    NOGUCHI Hiroshi

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    Grant amount:\41600000 ( Direct Cost: \32000000 、 Indirect Cost:\9600000 )

    Novel type III PKS was isolated from Garcinia mangostana L. , which contains mainly prenylated xanthones. A key prenyltransferase involved in the biosynthesis of abundant fungal meroterpenoids derived from 3,5-dimethylorsellinic was identified. The prenylated product serves as a key intermediate in the biosynthesis of series of meroterpenoids in fungi. With Aspergillus nidlans, Metabolic engineering of yeast for heterologous production of tryprostatins and other compounds was developed through our engineered strain of Saccharomyces cerevisiae, SCKW5. Further trial to architect comprehensive system to form secondary metabolites with Chaetomium globosum is under progress.

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  • ハイブリッド型天然物の生合成再構築による構造多様性の創製

    2009.4 - 2011.3

    若手研究(B) 

    久城哲夫

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\4550000 ( Direct Cost: \3500000 、 Indirect Cost:\1050000 )

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  • ハイブリッド型天然物による分子多様性の創製

    2008.4 - 2009.3

    持田記念医学薬学振興財団 

    久城哲夫

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\2000000 ( Direct Cost: \2000000 )

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  • Diversification of Molecular Structures by Merger of Biosynthetic Pathways

    Grant number:20241049  2008 - 2010

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

    EBIZUKA Yutaka, SHIBUYA Masaaki, KUSHIRO Tetsuo, ABE Ikurou

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    Grant amount:\30290000 ( Direct Cost: \23300000 、 Indirect Cost:\6990000 )

    By searching for gene-clusters that contain both putative PKS and PT in public gene database of filamentous fungi, two meroterpenoid gene clusters have been found and identified for the first time. All PKSs, PTs and CYCs found in this study show novel reactivity and specificity, and thus are expected to contribute for construction of structural diversity in a combinatorial fashion. Heterologous expression of the enzyme genes reconstituted a meroterpenoid biosynthesis. Further, a substrate directed biosynthesis afforded a non-natural meroterpenoid.

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  • トマトステロイドサポニンの生合成研究

    2007.4 - 2008.3

    武田科学振興財団 

    久城哲夫

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\2000000 ( Direct Cost: \2000000 )

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  • 植物におけるプロゲステロン生合成遺伝子の探索

    2005.4 - 2006.3

    上原記念生命科学財団 

    久城哲夫

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\2000000 ( Direct Cost: \2000000 )

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  • RNA干渉による重複遺伝子のサイレンシングを用いた植物P450の機能解析

    2004.4 - 2006.3

    若手研究(B) 

    久城哲夫

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\2900000 ( Direct Cost: \2900000 )

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  • Functional Genomics Approach to Exploiting Molecular Diversity

    Grant number:15101007  2003 - 2007

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (S)

    EBIZUKA Yutaka, SHIBUYA Masaaki, KUSHIRO Tetsuo

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    Grant amount:\95160000 ( Direct Cost: \73200000 、 Indirect Cost:\21960000 )

    (1) By utilizing genomic information, twenty four new triterpene synthase genes were cloned from higher plants, ferns and filamentous fungi. Their enzyme functions were identified by heterologous expression in yeast.
    (2) As the products of these triterpene synthases, three new triterpenes with novel skeletons were identified.
    (3) Lanosterol synthase genes were discovered for the first time from the plant kingdom and the presence of biosynthetic pathway of phytosterols via lanosterol was demonstrated.
    (4) By reacting triterpene synthases with non-physiological substrates and synthetic substrate analogues, five new polyprenoids were obtained
    (5) Each three novel oxidases and sugartransferases were cloned and their functions identified as the triterpene modifying enzymes.
    (6) Eleven new iterative polyketide synthase (i-PKS) genes were cloned from filamentous fungi. Their enzyme functions were identified by expression in a heterologous fungal strain.
    (7) Six new polyketide compounds with unique structures were identified as the products of these i-PKSs.
    (8) Four Type-III i-PKS genes were identified in the genome of Asp. oryzae and one of them was demonstrated to encode an orsellinic acid synthase.
    (9) As the higher structure for Type-I i-PKSs, a novel homo-tetramer model was proposed.
    (10) As the novel polyketide modifying enzymes, a chain-shortening enzyme, a dehydratase, an oxidase with cycloaddition activity and two prenyltrasferase were cloned and their enzyme functions identified.

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