Updated on 2026/03/07

写真a

 
HONDA MICHIYO
 
Organization
Undergraduate School School of Science and Technology Professor
Title
Professor
Homepage
https://kaken.nii.ac.jp/d/r/20384175.ja.html
External link

Research Interests

  • 組織工学

  • バイオマテリアル

  • 細胞

Research Areas

  • Life sciences / Cell biology

  • Life sciences / Regenerative dentistry and dental engineering

  • Life sciences / Biomedical engineering

  • Life sciences / Biomaterials

Research History

  • Meiji University   School of Science and Technology   Professor

    2021.4

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  • Meiji University   Associate Professor

    2016.4 - 2021.3

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  • Meiji University   Researcher

    2013.10 - 2016.3

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  • Kanagawa Academy of Science and Technology   Researcher

    2009.10 - 2013.9

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Papers

  • Dual-Target Antimicrobial Strategy Combining Cell-Penetrating Protamine Peptides and Membrane-Active ε-Poly-L-lysine

    Ryosuke Nakamura, Rie Togawa, Daisuke Koizumi, Masataka Kawarasaki, Keishi Iohara, Michiyo Honda

    Micro   2026.1

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/micro6010007

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  • FGF-2-Overexpressing Adipose-Derived Stem Cells as a Paracrine Platform for Angiogenesis-Driven Tissue Regeneration

    Daisuke Seki, Michiyo Honda

    CELLULAR AND MOLECULAR BIOENGINEERING   2025.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s12195-025-00883-w

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  • Loss of Calpain-2 attenuates TGF-β-driven Epithelial-Mesenchymal transition and invasive behavior in A549 lung cancer cells. International journal

    Himari Hara, Hiromichi Goshima, Daisuke Hoshino, Michiyo Honda

    Biochemical and biophysical research communications   790   152896 - 152896   2025.11

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    Lung cancer accounts for the highest number of cancer-related deaths globally, largely due to its high propensity for early and widespread metastasis. Calpains, a family of calcium-dependent cysteine proteases, are involved in various physiological processes, including cytoskeletal remodeling, migration, and invasion. Among them, calpain-2 has been implicated in promoting cancer cell invasiveness and metastatic behavior, although its precise role in non-small cell lung cancer (NSCLC) remains poorly understood. In this study, calpain-2-deficient cells were generated from the A549 lung adenocarcinoma cell line using CRISPR/Cas9 technology, and the effects of calpain-2 loss on cell migration, invasion, and epithelial-mesenchymal transition (EMT) were evaluated in comparison with control cells. We also evaluated the effects of transforming growth factor-β (TGF-β), a cytokine known to regulate diverse cellular processes, including proliferation, migration, and differentiation. Our results demonstrated that calpain-2 deficiency significantly reduced cell migratory capacity, EMT progression, and invasive potential following TGF-β stimulation. These effects were mediated by multiple molecular mechanisms, including impaired actin filament remodeling due to calpain-2 deficiency, reduced activation of Matrix Metalloproteinase (MMP)-2 and MMP-9, and decreased expression of EMT-related transcription factors such as Snail, Slug, and ZEB1. Collectively, these findings suggest that calpain-2 plays a central role in TGF-β-mediated cancer cell migration, invasion, and EMT progression, and that targeting calpain-2 function may represent a potential therapeutic strategy for inhibiting lung cancer metastasis.

    DOI： 10.1016/j.bbrc.2025.152896

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  • Polydopamine-Coating Nanoarchitectonics of Cellulose Hydrogels with Promoted Sustainable and Alkali-Resistant Natures for 3D Cell Culture Application. International journal

    Yurina Sekine, Michiyo Honda, Asako Sugita, Takuya Nankawa, Rimi Sakanaka, Tomoko Ikeda-Fukazawa, Katsuhiko Ariga

    ACS applied materials & interfaces   17 ( 37 )   52768 - 52778   2025.9

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    Sustainable and chemically resilient hydrogels are critically needed in biointerface engineering, particularly for 3D cell culture systems and surface modification under physiological to mildly alkaline conditions. However, physically cross-linked cellulose nanofiber (CNF) hydrogels─despite their renewable origin and biocompatibility─typically disintegrate at pH > 8, limiting their use in polydopamine (PDA)-based surface functionalization. Here, we present a simple and scalable physical treatment strategy to fabricate alkali-resistant, physically cross-linked hydrogels from carboxymethyl cellulose nanofibers (CMCF). By combining freeze-drying with mild thermal annealing (70 °C), the hydrogel network undergoes partial dehydration and ester bond formation via mild thermal condensation between carboxyl and hydroxyl groups, resulting in enhanced internal densification while preserving porosity and rehydration capacity. The resulting hydrogels remain structurally intact in Tris buffer (pH 9.7) and allow uniform in situ PDA coating at pH 8.5 on a physically cross-linked CMCF scaffold. The PDA-functionalized hydrogels exhibit interconnected porous microstructures, high water content (∼97 wt %), and robust mechanical and chemical stability. They support long-term 3D culture of EGFP-expressing human dermal fibroblasts for over 30 days, demonstrating excellent cytocompatibility and deep cell infiltration. This sustainable platform bridges green material design with functional surface engineering under alkaline conditions, and offers a versatile foundation for applications in regenerative medicine, selective ion adsorption, and environmental remediation.

    DOI： 10.1021/acsami.5c14529

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  • Preferred Orientation of Hydroxyapatite Ceramics Along the c-Axis Promotes Osteoblast Differentiation. International journal

    Kitaru Suzuki, Masaki Tamazawa, Erika Onuma, Michiyo Honda, Mamoru Aizawa

    International journal of molecular sciences   25 ( 23 )   2024.12

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    Hydroxyapatite (HAp) is similar to the main inorganic components of bone and tooth enamel. Furthermore, it possesses biocompatibility, making it suitable for clinical use in artificial bones. This study aimed to verify whether the preferred orientation of HAp influences osteogenesis. Using the templated grain growth method, we successfully fabricated HAp ceramics with a preferred orientation to m (a)-planes (aHAp) and examined the effects of this orientation on bone differentiation. Osteosarcoma-derived osteoblasts (MG-63) were cultured on aHAp and HAp ceramics made from commercially available powder (iHAp). Electron backscatter diffraction analysis revealed the crystal orientation distribution of HAp ceramics and the numerous exposed a-planes of aHAp. The MG-63 cultured on aHAp exhibited significantly higher alkaline phosphatase activity, a marker of early bone differentiation, compared to iHAp. Furthermore, the two-dimensional electrophoresis results indicated that the expressed proteins differed between aHAp and iHAp. These results indicate that controlling HAp's crystal structure may promote the osteogenic potential of osteoblasts. In this study, we propose that the a-plane of HAp promotes bone differentiation during the early stages, presenting a promising approach for novel biomaterials, such as high-performance artificial bones.

    DOI： 10.3390/ijms252312926

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  • "Rich arginine and strong positive charge" antimicrobial protein protamine: From its action on cell membranes to inhibition of bacterial vital functions. International journal

    Momoka Ookubo, Yuka Tashiro, Kosuke Asano, Yoshiharu Kamei, Yoshikazu Tanaka, Takayuki Honda, Takeshi Yokoyama, Michiyo Honda

    Biochimica et biophysica acta. Biomembranes   1866 ( 5 )   184323 - 184323   2024.6

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    Protamine, an antimicrobial protein derived from salmon sperm with a molecular weight of approximately 5 kDa, is composed of 60-70 % arginine and is a highly charged protein. Here, we investigated the mechanism of antimicrobial action of protamine against Cutibacterium acnes (C. acnes) focusing on its rich arginine content and strong positive charge. Especially, we focused on the attribution of dual mechanisms of antimicrobial protein, including membrane disruption or interaction with intracellular components. We first determined the dose-dependent antibacterial activity of protamine against C. acnes. In order to explore the interaction between bacterial membrane and protamine, we analyzed cell morphology, zeta potential, membrane permeability, and the composition of membrane fatty acid. In addition, the localization of protamine in bacteria was observed using fluorescent-labeled protamine. For investigation of the intracellular targets of protamine, bacterial translation was examined using a cell-free translation system. Based on our results, the mechanism of the antimicrobial action of protamine against C. acnes is as follows: 1) electrostatic interactions with the bacterial cell membrane; 2) self-internalization into the bacterial cell by changing the composition of the bacterial membrane; and 3) inhibition of bacterial growth by blocking translation inside the bacteria. However, owing to its strong electric charge, protamine can also interact with DNA, RNA, and other proteins inside the bacteria, and may inhibit various bacterial life processes beyond the translation process.

    DOI： 10.1016/j.bbamem.2024.184323

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  • The construction of a microenvironment with the vascular network by co-culturing fibroblasts and endothelial cells. International journal

    Tatsuwo Fujita, Taigo Yuki, Michiyo Honda

    Regenerative therapy   25   138 - 146   2024.3

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    INTRODUCTION: Extracellular matrix (ECM) synthesis and deposition in fibroblasts, and vascularization via endothelial cells are essential for successful tissue regeneration. Fibroblasts can produce both ECM, physical support for maintaining homeostasis, and bioactive molecules, such as growth factors and cytokines. Endothelial cells can secrete growth factors and form vascular networks that enable the supply of nutrients and oxygen and remove metabolic products. METHODS: In this study, we focused on combining Human Periodontal Ligament Fibroblasts (HPLF) and Human Umbilical Vein Endothelial Cells (HUVEC) for tissue regeneration in clinical applications. RESULTS: The fibroblastic and angiogenic phenotypes were promoted in co-culture with HPLF and HUVEC at a ratio of 1:1 compared to HPLF or HUVEC mono-culture. The gene expression of ECM components and angiogenesis-related factors was also enhanced by HPLF/HUVEC co-culture. Despite an apparent increase in the expression of angiogenic factors, the levels of secreted growth factors decreased under co-culture conditions. These data suggest that ECM constructed by HPLF and HUVEC would act as a storage site for growth factors, which can later be released. Our results showed that cell-to-cell interactions between HPLF and HUVEC enhanced collagen synthesis and endothelial network formation, leading to the creation of highly vascularized constructs for periodontal tissue regeneration. CONCLUSION: Successful periodontal tissue regeneration requires microenvironmental reconstruction and vascularization, which can be achieved using a co-culture system. In the present study, we found that fibroblastic and angiogenic phenotypes were enhanced by the co-culture of HPLF and HUVEC. The optimal culture conditions (1:1) could potentially accelerate tissue engineering, including ECM synthesis and EC tube formation, and these approaches can improve therapeutic efficacy after transplantation.

    DOI： 10.1016/j.reth.2023.12.004

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  • A Novel Control Method of Enterococcus faecalis by Co-Treatment with Protamine and Calcium Hydroxide. International journal

    Yu Abe, Michiyo Honda

    Pharmaceutics   15 ( 6 )   2023.5

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    Enterococcus faecalis (E. faecalis), a gram-positive facultative anaerobic bacterium, is likely to survive root canal treatment because of its extremely high alkaline tolerance, which may contribute to the refractory nature of apical periodontitis (AP). In this study, protamine was combined with calcium hydroxide to evaluate its efficacy in killing E. faecalis. First, the antibacterial activity of protamine against E. faecalis was investigated. Protamine reduced the E. faecalis growth rate at concentrations above the MIC (250 μg/mL), but was not bactericidal at any of the concentrations tested. Next, we investigated the calcium hydroxide tolerance of E. faecalis, using a 10% 310 medium, adjusted for pH by adding a calcium hydroxide solution. The results showed that E. faecalis could survive and proliferate in alkaline environments up to pH 10. However, the complete killing of E. faecalis was observed when protamine (250 μg/mL) was added. In addition, compared with treatment with protamine and calcium hydroxide alone, membrane damage and internalization of protamine into the cytoplasm of E. faecalis were enhanced. Therefore, the synergistic increase in antibacterial activity may be related to the action of both antimicrobial agents on the cell membrane. In conclusion, co-treatment with protamine and calcium hydroxide seems to be very effective in sterilizing E. faecalis, and has the potential to provide a novel control method against E. faecalis for root canal treatment.

    DOI： 10.3390/pharmaceutics15061629

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  • Wnt/β-Catenin Signaling Inhibits Osteogenic Differentiation in Human Periodontal Ligament Fibroblasts. International journal

    Ryoya Iizumi, Michiyo Honda

    Biomimetics (Basel, Switzerland)   7 ( 4 )   2022.12

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    The periodontal ligament is a collagenous tissue that is important for maintaining the homeostasis of cementum and alveolar bone. In tendon cells, Wnt/β-catenin signaling has been reported to regulate the expression level of Scleraxis (Scx) and Mohawk Homeobox (Mkx) gene and maintain the tissue homeostasis, while its role in the periodontal ligament is unclear. The aim of this study was to investigate the effects of Wnt/β-catenin signaling induced by Wnt-3a stimulation on the inhibition of osteogenic differentiation of human periodontal ligament fibroblasts (HPLFs). During osteogenic differentiation of HPLFs, they formed bone nodules independently of alkaline phosphatase (ALP) activity. After stimulation of Wnt-3a, the expression of β-catenin increased, and nuclear translocation of β-catenin was observed. These data indicate that Wnt-3a activated Wnt/β-catenin signaling. Furthermore, the stimulation of Wnt-3a inhibited the bone nodule formation and suppressed the expression of osteogenic differentiation-related genes such as Runx2, Osteopontin and Osteocalcin, and upregulated the gene expression of Type-I collagen and Periostin (Postn). Scx may be involved in the suppression of osteogenic differentiation in HPLFs. In conclusion, Wnt/β-catenin signaling may be an important signaling pathway that inhibits the osteogenic differentiation in HPLFs by the upregulation of Scx gene expression and downregulation of osteogenic differentiation-related genes.

    DOI： 10.3390/biomimetics7040224

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  • Preparation of antimicrobial calcium phosphate/protamine composite powders with fluoride ions using octacalcium phosphate. International journal

    Daisuke Koizumi, Kitaru Suzuki, Rie Togawa, Kosuke Yasui, Keishi Iohara, Michiyo Honda, Mamoru Aizawa

    Journal of materials science. Materials in medicine   33 ( 4 )   35 - 35   2022.4

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    Calcium phosphates are key biomaterials in dental treatment and bone regeneration. Biomaterials must exhibit antibacterial properties to prevent microbial infection in implantation frameworks. Previously, we developed various types of calcium phosphate powders (amorphous calcium phosphate, octacalcium phosphate (OCP), dicalcium phosphate anhydrate, and hydroxyapatite) with adsorbed protamine (which is a protein with antibacterial property) and confirmed their antibacterial property. In this study, as foundational research for the development of novel oral care materials, we synthesized calcium phosphate composite powders from three starting materials: i) OCP, which intercalates organic compounds, ii) protamine, which has antibacterial properties, and iii) F- ion, which promotes the formation of apatite crystals. Through investigating the preparation concentration of the F- ions and their loading into OCP, it was found that more F- ion could be loaded at higher concentrations regardless of the loading method. It was also observed that the higher the preparation concentration, the more the OCP converted to fluorapatite. The synthesized calcium phosphate composite powders were evaluated for biocompatibility through proliferation of MG-63 cells, with none of the powders exhibiting any growth inhibition. Antimicrobial tests showed that the calcium phosphate composite powders synthesized with protamine and F- ion by precipitation had enhanced antimicrobial properties than those synthesized by protamine adsorption. Thus, the calcium phosphate composite powder prepared from OCP, protamine, and F- ion forms the basis for promising antimicrobial biomaterials. Graphical abstract.

    DOI： 10.1007/s10856-022-06656-5

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  • Preparation of protamine-adsorbed calcium phosphate powders and their antibacterial property

    Daisuke Koizumi, Kitaru Suzuki, Hirogo Minamisawa, Rie Togawa, Kosuke Yasui, Keishi Iohara, Michiyo Honda, Mamoru Aizawa

    Journal of Asian Ceramic Societies   10 ( 1 )   230 - 240   2022.1

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    Publishing type：Research paper (scientific journal)   Publisher：Informa UK Limited  

    DOI： 10.1080/21870764.2022.2035488

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  • Living reactions of tissue-engineered bone derived from apatite-fiber scaffold in rat subcutaneous tissues

    Kitaru Suzuki, Michiyo Honda, Tomokazu Matsuura, Mamoru Aizawa

    JOURNAL OF THE CERAMIC SOCIETY OF JAPAN   130 ( 1 )   65 - 73   2022.1

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    DOI： 10.2109/jcersj2.21108

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  • Influence of Culture Period on Osteoblast Differentiation of Tissue-Engineered Bone Constructed by Apatite-Fiber Scaffolds Using Radial-Flow Bioreactor. International journal

    Kitaru Suzuki, Jun Fukasawa, Maiko Miura, Poon Nian Lim, Michiyo Honda, Tomokazu Matsuura, Mamoru Aizawa

    International journal of molecular sciences   22 ( 23 )   2021.12

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    DOI： 10.3390/ijms222313080

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  • Moderate Hypothermia Has the Potential to Reveal the Dominant/Submissive Relationship in a Co-Culture System Consisting of Osteoblasts and Endothelial Cells

    Kouki Inomata, Michiyo Honda

    Micro   2021.9

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/micro1020014

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  • Regulating size of silver nanoparticles on calcium carbonate via ultrasonic spray for effective antibacterial efficacy and sustained release. International journal

    Mayu Ueda, Tomohiro Yokota, Michiyo Honda, Poon Nian Lim, Naoya Osaka, Masashi Makita, Yasutoshi Nishikawa, Toshihiro Kasuga, Mamoru Aizawa

    Materials science & engineering. C, Materials for biological applications   125   112083 - 112083   2021.6

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    DOI： 10.1016/j.msec.2021.112083

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  • Zoledronic Acid-Loaded β-TCP Inhibits Tumor Proliferation and Osteoclast Activation: Development of a Functional Bone Substitute for an Efficient Osteosarcoma Treatment. International journal

    Yuka Kameda, Mamoru Aizawa, Taira Sato, Michiyo Honda

    International journal of molecular sciences   22 ( 4 )   2021.2

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    Osteosarcoma has a poor survival rate due to relapse and metastasis. Zoledronic acid (ZOL), an anti-resorptive and anti-tumor agent, is used for treating osteosarcoma. Delivery of ZOL to the target region is difficult due to its high binding affinity to bone minerals. This study developed a novel treatment for osteosarcoma by delivering ZOL to the target region locally and sustainably. In this study, we fabricated a novel bone substitute by loading ZOL on β-tricalcium phosphate (β-TCP). The ZOL-loaded β-TCP (ZOL/β-TCP) would be expected to express the inhibitory effects via both bound-ZOL (bound to β-TCP) and free-ZOL (release from ZOL/β-TCP). To explore the ability to release ZOL from the ZOL/β-TCP, the amount of released ZOL was measured. The released profile indicates that a small amount of ZOL was released, and most of it remained on the β-TCP. Our data showed that ZOL/β-TCP could successfully express the effects of ZOL via both bound-ZOL and free-ZOL. In addition, we examined the biological effects of bound/free-ZOL using osteosarcoma and osteoclasts (target cells). The results showed that two states of ZOL (bound/free) inhibit target cell activities. As a result, ZOL/β-TCP is a promising candidate for application as a novel bone substitute.

    DOI： 10.3390/ijms22041889

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  • Pigs with delta-sarcoglycan deficiency exhibit traits of genetic cardiomyopathy Reviewed International journal

    Hitomi Matsunari, Michiyo Honda, Masahito Watanabe, Satsuki Fukushima, Kouta Suzuki, Shigeru Miyagawa, Kazuaki Nakano, Kazuhiro Umeyama, Ayuko Uchikura, Kazutoshi Okamoto, Masaki Nagaya, Teruhiko Toyo-oka, Yoshiki Sawa, Hiroshi Nagashima

    LABORATORY INVESTIGATION   100 ( 6 )   887 - 899   2020.6

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    DOI： 10.1038/s41374-020-0406-7

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  • Potential Application of Protamine for Antimicrobial Biomaterials in Bone Tissue Engineering Reviewed International journal

    Michiyo Honda, Morio Matsumoto, Mamoru Aizawa

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   21 ( 12 )   2020.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/ijms21124368

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  • Bactericidal and Bioresorbable Calcium Phosphate Cements Fabricated by Silver-Containing Tricalcium Phosphate Microspheres Reviewed International journal

    Michiyo Honda, Yusuke Kawanobe, Kohei Nagata, Ken Ishii, Morio Matsumoto, Mamoru Aizawa

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   21 ( 11 )   2020.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/ijms21113745

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  • Pigs with delta-sarcoglycan deficiency exhibit traits of genetic cardiomyopathy (Feb, 10.1038/s41374-020-0406-7, 2020) Reviewed International journal

    Hitomi Matsunari, Michiyo Honda, Masahito Watanabe, Satsuki Fukushima, Kouta Suzuki, Shigeru Miyagawa, Kazuaki Nakano, Kazuhiro Umeyama, Ayuko Uchikura, Kazutoshi Okamoto, Masaki Nagaya, Teruhiko Toyo-oka, Yoshiki Sawa, Hiroshi Nagashima

    LABORATORY INVESTIGATION   100 ( 6 )   900 - 900   2020.6

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  • The investigation of synergistic activity of protamine with conventional antimicrobial agents against oral bacteria Reviewed International journal

    Masashi Fujiki, Michiyo Honda

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   523 ( 3 )   561 - 566   2020.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bbrc.2020.01.002

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  • Fabrication of Gentamicin-Loaded Hydroxyapatite/Collagen Bone-Like Nanocomposite for Anti-Infection Bone Void Fillers Reviewed International journal

    Sho Oshima, Taira Sato, Michiyo Honda, Yasushi Suetsugu, Kazuhide Ozeki, Masanori Kikuchi

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   21 ( 2 )   2020.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/ijms21020551

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  • Development of nitrogen-doped hydroxyapatite ceramics Reviewed

    Nao Kaneko, Yuhei Suzuki, Ryo Umeda, Ryota Namiki, Chihiro Izawa, Tomoko Ikeda Fukazawa, Michiyo Honda, Takahiro Takei, Tomoaki Watanabe, Mamoru Aizawa

    JOURNAL OF ASIAN CERAMIC SOCIETIES   8 ( 1 )   130 - 137   2020.1

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    DOI： 10.1080/21870764.2020.1712799

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  • Antimicrobial Activity of Protamine-Loaded Calcium Phosphates against Oral Bacteria. Reviewed International journal

    Masashi Fujiki, Kodai Abe, Tohru Hayakawa, Takatsugu Yamamoto, Mana Torii, Keishi Iohara, Daisuke Koizumi, Rie Togawa, Mamoru Aizawa, Michiyo Honda

    Materials (Basel, Switzerland)   12 ( 17 )   2019.9

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    Protamine is an antimicrobial peptide extracted from fish. In this study, we loaded protamine onto dicalcium phosphate anhydride (DCPA), a dental material. Protamine was loaded by stirring DCPA into a protamine solution. To explore the antimicrobial activity of the materials, we cultivated Streptococcus mutans on fabricated discs for 24 h. When S. mutans was cultivated on the discs under no sucrose conditions, the loaded protamine was not released, and the ratio of dead bacteria increased on the surface of P (125) DCPA (half of the saturated level of protamine (125 ppm protamine) was loaded). Aside from P (500) DCPA (saturated level of protamine was loaded), some protamine was released, and the number of planktonic bacteria in the supernatant decreased. Using medium containing 1% sucrose, the release of protamine was promoted from P (125) DCPA due to lowered pH. However, lowering of the pH decreased the antimicrobial activity of protamine. On the other hand, P (500) DCPA released protamine before the pH was lowered, and biofilm formation was inhibited. The loaded protamine expressed antimicrobial activity, both on the surface of the materials and in the surrounding environment. The interaction of loaded protamine with calcium phosphates could promote the application of protamine in the dental field.

    DOI： 10.3390/ma12172816

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  • Co-Culture of Osteoblasts and Endothelial Cells on a Microfiber Scaffold to Construct Bone-Like Tissue with Vascular Networks Reviewed International journal

    Kouki Inomata, Michiyo Honda

    MATERIALS   12 ( 18 )   2019.9

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    DOI： 10.3390/ma12182869

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  • Acceleration of Osteogenesis via Stimulation of Angiogenesis by Combination with Scaffold and Connective Tissue Growth Factor Reviewed International journal

    Michiyo Honda, Ryo Hariya, Morio Matsumoto, Mamoru Aizawa

    MATERIALS   12 ( 13 )   2019.7

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    DOI： 10.3390/ma12132068

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  • Distributions of endocrine cell clusters during porcine pancreatic development Reviewed International journal

    Masaki Nagaya, Asuka Hayashi, Kazuaki Nakano, Michiyo Honda, Koki Hasegawa, Kazutoshi Okamoto, Shiori Itazaki, Hitomi Matsunari, Masahito Watanabe, Kazuhiro Umeyama, Hiroshi Nagashima

    PLOS ONE   14 ( 5 )   e0216254   2019.5

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    DOI： 10.1371/journal.pone.0216254

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  • Fabrication of chelate-setting alpha-tricalcium phosphate cement using sodium citrate and sodium alginate as mixing solution and its in vivo osteoconductivity Reviewed International journal

    Toshiisa Konishi, Poon Nian Lim, Michiyo Honda, Masaki Nagaya, Hiroshi Nagashima, Eng San Thian, Mamoru Aizawa

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS   106 ( 6 )   2361 - 2370   2018.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/jbm.b.34028

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  • Fabrication and biological evaluation of hydroxyapatite ceramics including bone minerals Reviewed

    Tomohiro Yokota, Takuya Miki, Michiyo Honda, Tomoko Ikeda-Fukazawa, Ken Ishii, Morio Matsumoto, Mamoru Aizawa

    JOURNAL OF THE CERAMIC SOCIETY OF JAPAN   126 ( 2 )   99 - 108   2018.2

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    DOI： 10.2109/jcersj2.17216

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  • Bioresorbability of chelate-setting calcium-phosphate cement hybridized with gelatin particles using a porcine tibial defect model Reviewed

    Keishi Kiminami, Kohei Nagata, Toshiisa Konishi, Minori Mizumoto, Michiyo Honda, Kazuaki Nakano, Masaki Nagaya, Hidetoshi Arimura, Hiroshi Nagashima, Mamoru Aizawa

    JOURNAL OF THE CERAMIC SOCIETY OF JAPAN   126 ( 2 )   71 - 78   2018.2

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    DOI： 10.2109/jcersj2.17197

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  • In vivo evaluation of porous hydroxyapatite ceramics including bone minerals using pig model Reviewed

    Tomohiro Yokota, Kazuaki Nakano, Masaki Nagaya, Michiyo Honda, Hiroshi Nagashima, Mamoru Aizawa

    MATERIALS TECHNOLOGY   33 ( 10 )   689 - 697   2018

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    DOI： 10.1080/10667857.2018.1495392

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  • Effects of Adding Polysaccharides and Citric Acid into Sodium Dihydrogen Phosphate Mixing Solution on the Material Properties of Gelatin-Hybridized Calcium-Phosphate Cement Reviewed International journal

    Keishi Kiminami, Toshiisa Konishi, Minori Mizumoto, Kohei Nagata, Michiyo Honda, Hidetoshi Arimura, Mamoru Aizawa

    MATERIALS   10 ( 8 )   2017.8

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    DOI： 10.3390/ma10080941

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  • Injectable chelate-setting hydroxyapatite cement prepared by using chitosan solution: Fabrication, material properties, biocompatibility, and osteoconductivity Reviewed International journal

    Toshiisa Konishi, Michiyo Honda, Masaki Nagaya, Hiroshi Nagashima, Eng San Thian, Mamoru Aizawa

    JOURNAL OF BIOMATERIALS APPLICATIONS   31 ( 10 )   1319 - 1327   2017.5

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  • Evaluation of resistance to fragmentation of injectable calcium-phosphate cement paste using X-ray microcomputed tomography Reviewed

    Kohei Nagata, Kei Fujioka, Toshiisa Konishi, Michiyo Honda, Masaki Nagaya, Hiroshi Nagashima, Mamoru Aizawa

    JOURNAL OF THE CERAMIC SOCIETY OF JAPAN   125 ( 1 )   1 - 6   2017.1

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    DOI： 10.2109/jcersj2.16199

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  • Fabrication and characterization of chelate-setting β-tricalcium phosphate cements with enhanced bioresorbability Reviewed

    Kohei Nagata, Toshiisa Konishi, Michiyo Honda, Mamoru Aizawa

    Key Engineering Materials   720   157 - 161   2017

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    DOI： 10.4028/www.scientific.net/KEM.720.157

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  • EFFECTS OF ADDITION OF α-TRICALCIUM PHOSPHATE POWDERS ON MATERIAL PROPERTIES OF THE CHELATE-SETTING HYDROXYAPATITE CEMENT

    Kiminami Keishi, Matsuoka Kento, Konishi Toshiisa, Mizumoto Minori, Honda Michiyo, Arimura Hidetoshi, Aizawa Mamoru

    Phosphorus Research Bulletin   33   7 - 13   2017

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    We have previously developed a chelate-setting calcium-phosphate cement (CPC) consisting of hydroxyapatite (HAp) powders surface-modified with inositol hexaphosphate (IP6). In the present study, mechanically ball-milled α-tricalcium phosphate (α-TCP) powders were added into the IP6-HAp cement in order to enhance its anti-washout capability and mechanical properties. After optimization of the ball-milling conditions, the cement specimens mixed with various amounts of the prepared α-TCP powders were fabricated. Examinations of their mechanical properties revealed significant increase of compressive strength (CS) with the addition of the α-TCP powders. Further, it was demonstrated that the specimens of the IP6-HAp powders / the α-TCP powders mixing ratios of 20/80 and 30/70 [g/g] exhibited comparatively high anti-washout capability. In conclusion, these cements are promising chelate-setting CPCs with enhanced anti-washout capability and mechanical properties.

    DOI： 10.3363/prb.33.7

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  • Histological evaluations of apatite-fiber scaffold cultured with mesenchymal stem cells by implantation at rat subcutaneous tissue Reviewed International journal

    Kitaru Suzuki, Kohei Nagata, Tomohiro Yokota, Michiyo Honda, Mamoru Aizawa

    BIO-MEDICAL MATERIALS AND ENGINEERING   28 ( 1 )   57 - 64   2017

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  • Synthesis of Calcium Phosphate Microspheres Using an Ultrasonic Spray-Pyrolysis Technique and Their Application as Novel Anti-Angiogenic Chemoembolization Agents for Cancer Treatment Reviewed

    Mamoru Aizawa, Michiyo Honda, Makoto Emoto

    ADVANCES IN BIOINSPIRED AND BIOMEDICAL MATERIALS, VOL 2   1253   107 - 121   2017

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  • Chemical state of nitrogen in nitrogen-doped hydroxyapatite ceramics with enhanced bioactivity Reviewed

    R. Namiki, T. Suyama, C. Izawa, T. Ikeda-Fukazawa, M. Honda, T. Watanabe, M. Aizawa

    Key Engineering Materials   720   215 - 218   2017

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  • Preliminary study for co-culture of osteoblasts and endothelial cells to construct the regenerative bone Reviewed

    Michiyo Honda, Mamoru Aizawa

    Key Engineering Materials   758   269 - 272   2017

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    DOI： 10.4028/www.scientific.net/KEM.758.269

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  • Preparation and characterization of β-Tricalcium phosphate powders with high solubility for chelate-setting calcium-phosphate cements Reviewed

    Kohei Nagata, Toshiisa Konishi, Michiyo Honda, Mamoru Aizawa

    Key Engineering Materials   758   194 - 198   2017

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  • Fabrication of Potassium-Substituted Hydroxyapatite Ceramics via Ultrasonic Spray-Pyrolysis Route Reviewed

    Tomohiro Yokota, Michiyo Honda, Mamoru Aizawa

    Phosphorus Res. Bull   33   35 - 40   2017

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    Biological apatite presented in bone and teeth of mammals contains various ions, such as Na<SUP>+</SUP>, K<SUP>+</SUP>, Mg<SUP>2+</SUP>, F<SUP>-</SUP> and CO<SUB>3</SUB><SUP>2-</SUP> ions, in trace levels. Substitution of the above ions into hydroxyapatite (HAp) have great effect on the crystallinity, morphology, lattice parameters and stability of the apatite structure. It is known that potassium (K) in the living bone give a tremendous effect to the biomineralization process. Aim of this study is to clarify the influence of substitution of potassium into the HAp structure on the properties of powders and ceramics. In this study, we have fabricated the potassium-substituted hydroxyapatite (KAp) ceramics via an ultrasonic spray-pyrolysis route. Effect of potassium substitution on the phase, chemical composition, morphology and crystal lattice structure of HA was examined. Experimental results showed that the addition of potassium does not significantly affect the crystal phase, particle morphology and particle size. Sintered bodies fabricated from KAp powders were of single phase of HAp, and lattice constants of a-axis and c-axis increased with potassium contents. Substitution of potassium into the HAp lattice formed OH vacancies and caused grain growth.

    DOI： 10.3363/prb.33.35

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  • Fabrication of sodium-substituted hydroxyapatite ceramics via ultrasonic spray-pyrolysis route and their material properties Reviewed

    Tomohiro Yokota, Rina Ito, Yusuke Shimizu, Michiyo Honda, Mamoru Aizawa

    Key Engineering Materials   758   166 - 171   2017

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  • Preparation of Spherical Zn-Substituted Tricalcium Phosphate Powder by Ultrasonic Spray-Pyrolysis Technique and Its Characterization Reviewed

    Hiroki Nishikawa, Michiyo Honda, Tomohiro Yokota, Yusuke Shimizu, Mamoru Aizawa

    JOURNAL OF NANOMATERIALS   2016   2016

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    DOI： 10.1155/2016/6753203

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  • A New System to Evaluate the Influence of Immunosuppressive Drugs on Pancreatic Islets Using Epigenetic Analysis in a 3-Dimensional Culture Reviewed International journal

    Masaki Nagaya, Yoshikazu Arai, Hitomi Matsunari, Michiyo Honda, Kazuaki Nakano, Miki Maehara, Naomi Sugimoto, Mirina Kobayashi, Rieko Sakai, Yoshinori Asano, Masahito Watanabe, Kazuhiro Umeyama, Hiroshi Nagashima

    PANCREAS   44 ( 5 )   778 - 785   2015.7

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  • Antibacterial polyetheretherketone implants immobilized with silver ions based on chelate-bonding ability of inositol phosphate: Processing, material characterization, cytotoxicity, and antibacterial properties

    H. Kakinuma, K. Ishii, H. Ishihama, M. Honda, Y. Toyama, M. Matsumoto, M. Aizawa

    Journal of Biomedical Materials Research - Part A   103 ( 1 )   57 - 64   2015.1

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    DOI： 10.1002/jbm.a.35157

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  • In vivo evaluation of chelate-Setting cement fabricated from hydroxyapatite including bone minerals using a rabbit's tibia model

    Mamoru Aizawa, Yuki Chibu, Kohei Nagata, Toshiisa Konishi, Ken Ishii, Haruki Funao, Yoshiaki Toyama, Morio Matsumoto, Michiyo Honda

    Key Engineering Materials   631   402 - 407   2015

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    DOI： 10.4028/www.scientific.net.KEM.631.402

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  • Development of bioresorbable calcium-Phosphate cements hybridized with gelatin particles and their in vivo evaluation using pig's tibia model Reviewed

    Keishi Kiminami, Kento Matsuoka, Kohei Nagata, Toshiisa Konishi, Michiyo Honda, Gota Hayashida, Kazuaki Nakano, Masaki Nagaya, Hidetoshi Arimura, Hiroshi Nagashima, Mamoru Aizawa

    Key Engineering Materials   631   397 - 401   2015

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    DOI： 10.4028/www.scientific.net.KEM.631.397

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  • Preparation of α-Tricalcium Phosphate Powders Surface-modified with Inositol Phosphate for Cement Fabrication Reviewed

    Toshiisa Konishi, Michiyo Honda, Tomohiko Yoshioka, Satoshi Hayakawa, Mamoru Aizawa

    Key Engineering Materials   631   113 - 118   2015

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    DOI： 10.4028/www.scientific.net.KEM.631.113

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  • Biocompatibility of silver-Containing calcium-Phosphate cements with anti-Bacterial properties Reviewed

    Yusuke Shimizu, Yusuke Kawanobe, Toshiisa Konishi, Nobuyuki Kanzawa, Michiyo Honda, Mamoru Aizawa

    Key Engineering Materials   631   107 - 112   2015

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    DOI： 10.4028/www.scientific.net.KEM.631.107

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  • Biocompatibility of silver-containing calcium-phosphate cements with anti-bacterial properties

    Yusuke Shimizu, Yusuke Kawanobe, Toshiisa Konishi, Nobuyuki Kanzawa, Michiyo Honda, Mamoru Aizawa

    Key Engineering Materials   631   107 - 112   2014.11

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  • Fabrication of apatie-fiber scaffolds with enhanced mechanical property using chemical cross-linking of type I-collagen and their biocompatibility. Reviewed

    Satoshi MOTOJIMA, Naoko IGETA, Hiroki MURAMATSU, Hiroshi UCHIDA, Kiyoshi ITATANI, Nobuyuki KANZAWA, Michiyo HONDA, Mamoru AIZAWA

    Journal of the Society of Inorganic Materials, Japan   21 ( 372 )   278 - 285   2014.9

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  • In vitro and in vivo antimicrobial properties of silver-containing hydroxyapatite prepared via ultrasonic spray pyrolysis route Reviewed International journal

    Michiyo Honda, Yusuke Kawanobe, Ken Ishii, Toshiisa Konishi, Minori Mizumoto, Nobuyuki Kanzawa, Morio Matsumoto, Mamoru Aizawa

    MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS   33 ( 8 )   5008 - 5018   2013.12

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    DOI： 10.1016/j.msec.2013.08.026

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  • Selective differentiation of bone marrow-derived mesenchymal stromal cells into osteocytes via endochondral ossification in an apatite-fiber scaffold Reviewed

    Michiyo Honda, Yuka Watanabe, Takahide Tsuchiya, Nobuyuki Kanzawa, Mamoru Aizawa

    JOURNAL OF THE CERAMIC SOCIETY OF JAPAN   121 ( 1417 )   759 - 765   2013.9

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    DOI： 10.2109/jcersj2.121.759

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  • Selective differentiation of bone marrow-derived mesenchymal stromal cells into osteocytes via endochondral ossification in an apatite-fiber scaffold.

    Honda M, Watanabe Y, Tsuchiya T, Kanzawa N, Aizawa M

    Journal of the Ceramic Society of Japan   121 ( 1417 )   759 - 765   2013.7

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  • Biodegradable beta-tricalcium phosphate cement with anti-washout property based on chelate-setting mechanism of inositol phosphate Reviewed International journal

    Toshiisa Konishi, Shuhei Takahashi, Zhi Zhuang, Kohei Nagata, Minori Mizumoto, Michiyo Honda, Yasuhiro Takeuchi, Hitomi Matsunari, Hiroshi Nagashima, Mamoru Aizawa

    JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE   24 ( 6 )   1383 - 1394   2013.6

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    DOI： 10.1007/s10856-013-4903-8

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  • Novel chelate-setting calcium-phosphate cements fabricated with wet-synthesized hydroxyapatite powder Reviewed International journal

    Toshiisa Konishi, Yukiko Horiguchi, Minori Mizumoto, Michiyo Honda, Kazuya Oribe, Hikaru Morisue, Ken Ishii, Yoshiaki Toyama, Morio Matsumoto, Mamoru Aizawa

    JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE   24 ( 3 )   611 - 621   2013.3

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  • Fabrication of Novel Biodegradable alpha-Tricalcium Phosphate Cement Set by Chelating Capability of Inositol Phosphate and Its Biocompatibility Reviewed

    Toshiisa Konishi, Minori Mizumoto, Michiyo Honda, Yukiko Horiguchi, Kazuya Oribe, Hikaru Morisue, Ken Ishii, Yoshiaki Toyama, Morio Matsumoto, Mamoru Aizawa

    JOURNAL OF NANOMATERIALS   2013   2013

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  • Evaluation of the Anti-Bacterial Activity of a Novel Chelate-Setting Apatite Cement Containing Lactoferrin Reviewed

    Hitomi Ohsugi, Yuri Habuto, Michiyo Honda, Mamoru Aizawa, Nobuyuki Kanzawa

    BIOCERAMICS 24   529-530   187 - +   2013

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    DOI： 10.4028/www.scientific.net/KEM.529-530.187

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  • In Vitro Evaluation of Chelate-Setting Cements Fabricated from Silicon-Containing Apatite Powder Using Osteoblastic Cells Reviewed

    Yusuke Nakashima, Michiyo Honda, Toshiisa Konishi, Minori Mizumoto, Mamoru Aizawa

    BIOCERAMICS 24   529-530   183 - +   2013

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  • In Vitro Biological Evaluation of Anti-Tumor Effect of the Chelate-Setting Hydroxyapatite Cement Reviewed

    Michiyo Honda, Toshiisa Konishi, Minori Mizumoto, Mamoru Aizawa

    BIOCERAMICS 24   529-530   173 - 177   2013

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  • Comparative Study on Bioresorbability of Chelate-setting Cements with Various Calcium-Phosphate Phase Using Rabbit Model Reviewed

    Toshiisa Konishi, Shuhei Takahashi, Minori Mizumoto, Michiyo Honda, Koki Kida, Yukiko Horiguchi, Kazuya Oribe, Ken Ishii, Hikaru Morisue, Yoshiaki Toyama, Morio Matsumoto, Mamoru Aizawa

    BIOCERAMICS 24   529-530   167 - +   2013

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  • Adsorption Behavior of Sodium Inositol Hexaphosphate on the Surface of Hydroxyapatite Reviewed

    Toshiisa Konishi, Minori Mizumoto, Michiyo Honda, Mamoru Aizawa

    BIOCERAMICS 24   529-530   161 - 166   2013

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  • Enhanced early osteogenic differentiation by silicon-substituted hydroxyapatite ceramics fabricated via ultrasonic spray pyrolysis route Reviewed International journal

    Michiyo Honda, Koichi Kikushima, Yusuke Kawanobe, Toshiisa Konishi, Minori Mizumoto, Mamoru Aizawa

    JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE   23 ( 12 )   2923 - 2932   2012.12

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    DOI： 10.1007/s10856-012-4744-x

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  • Fabrication of chelate-setting cement from hydroxyapatite powder prepared by simultaneously grinding and surface-modifying with sodium inositol hexaphosphate and their material properties Reviewed

    Toshiisa Konishi, Zhi Zhuang, Minori Mizumoto, Michiyo Honda, Mamoru Aizawa

    JOURNAL OF THE CERAMIC SOCIETY OF JAPAN   120 ( 1401 )   159 - 165   2012.5

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  • Fabrication of Chelate-setting Cements from Hydroxyapatite Powders Surface-modified with Various Sodium Inositol Hexaphosphate Concentrations and their Mechanical Properties Reviewed

    Toshiisa Konishi, Minori Mizumoto, Michiyo Honda, Zhi Zhuang, Mamoru Aizawa

    IUMRS INTERNATIONAL CONFERENCE IN ASIA 2011   36   137 - 143   2012

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    DOI： 10.1016/j.proeng.2012.03.021

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  • Preparation of silver-containing hydroxyapatite powder by ultrasonic spray-pyrolysis technique and its antibacterial property

    Yusuke Kawanobe, Michiyo Honda, Toshiisa Konishi, Minori Mizumoto, Yuri Habuto, Nobuyuki Kanzawa, Zhi Zhuang, Mamoru Aizawa

    Key Engineering Materials   493-494   678 - 683   2012

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    DOI： 10.4028/www.scientific.net/KEM.493-494.678

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  • Biochemical studies of the potential anti-tumor activity of novel chelate-setting apatite cements

    Hidenori Konishi, Michiyo Honda, Mamoru Aizawa, Nobuyuki Kanzawa

    Key Engineering Materials   493-494   315 - 319   2012

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  • Effect of the addition of various polysaccharides on the material properties and cytotoxicity of chelate-setting β-tricalcium phosphate cement

    T. Konishi, S. Takahashi, M. Mizumoto, M. Honda, K. Oribe, M. Aizawa

    Phosphorus Res. Bull   26   59-64   2012

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    DOI： 10.3363/prb.26.59

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  • In vitro bioresorbability of chelate-setting cemetns with various calcium-phosphate phases

    H. Manabe, T. Konishi, M. Mizumoto, M. Honda

    Phosphorus Res. Bull   26   105-108   2012

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  • In vivo biocompatibility of apatite-fiber scaffold with enhanced mechanical property using pig model by implanting into tibia

    T. Ganmoto, M. Honda, T. Konishi, M. Mizumoto, H. Matsunari, Y. Takeuchi, H. Nagashima

    Phosphorus Res. Bull   26   1-3   2012

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  • Fabrication of chelate-setting cement using silicon-substituted hydroxyapatite and its property Reviewed

    Y. Nakashima, M. Honda, T. Konishi, M. Mizumoto, M. Aizawa

    Key Engineering Materials   493-494 ( 493 )   370 - 374   2012

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  • Preparation of apatite microspheres with nano-size pores on the surface via salt-assisted ultrasonic spray-pyrolysis technique and its drug release behavior

    Kawanobe Y, Honda M, Konishi T, Mizumoto M, Habuto Y, Kanzawa N, Aizawa M

    Journal of the Australian Ceramics Society   47 ( 1 )   6 - 10   2011.3

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  • High Molecular Weight Lectin Isolated from the Mucus of the Giant African Snail Achatina fulica Reviewed International journal

    Shigeru Ito, Masahiro Shimizu, Maki Nagatsuka, Seiji Kitajima, Michiyo Honda, Takahide Tsuchiya, Nobuyuki Kanzawa

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   75 ( 1 )   20 - 25   2011.1

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  • Fabrication of novel bioresorbable β-tricalcium phosphate cement on the basis of chelate-setting mechanism of inositol phosphate and its evaluation Reviewed

    Shuhei Takahashi, Toshiisa Konishi, Koji Nishiyama, Minori Mizumoto, Michiyo Honda, Yukiko Horiguchi, Kazuya Oribe, Mamoru Aizawa

    Journal of the Ceramic Society of Japan   119 ( 1385 )   35 - 42   2011

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    DOI： 10.2109/jcersj2.119.35

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  • Cell Proliferation, Morphology and Differentiation of Transgenic-Cloned Pig Calvarial Osteoblasts on the Silicon-Substituted Hydorxyapatite Ceramics Fabricated via Ultrasonic Spray-Pyrolysis Technique Reviewed

    M. Honda, K. Kikushima, T. Konishi, M. Mizumoto, H. Matsunari, H. Nagashima, M. Aizawa

    JOURNAL OF THE AUSTRALIAN CERAMIC SOCIETY   47 ( 1 )   37 - 41   2011

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  • Topographical analyses of proliferation and differentiation of osteoblasts in micro- and macropores of apatite-fiber scaffold Reviewed

    Michiyo Honda, Takahiko J. Fujimi, Shigeki Izumi, Kouji Izawa, Mamoru Aizawa, Hikaru Morisue, Takahide Tsuchiya, Nobuyuki Kanzawa

    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A   94A ( 3 )   937 - 944   2010.9

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  • A study on the bone induction of mouse C2C12 myoblast cultured in apatite-fiber scaffold.

    Maeda K, Honda M, Tsuchiya T, Aizawa M, Kanzawa N

    Bioceramics   22   435 - 438   2009.10

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  • Analyses of ossification of bone marrow stromal cells on apatite-fiber scaffolds

    Honda M, Watanabe Y, Aizawa M, Kanzawa N

    Bioceramics   22   205 - 208   2009.10

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  • Specific knockdown of delta-sarcoglycan gene in C2C12 in vitro causes post-translational loss of other sarcoglycans without mechanical stress Reviewed

    Michiyo Honda, Mari Hosoda, Nobuyuki Kanzawa, Takahide Tsuchiya, Teruhiko Toyo-oka

    MOLECULAR AND CELLULAR BIOCHEMISTRY   323 ( 1-2 )   149 - 159   2009.3

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    DOI： 10.1007/s11010-008-9975-2

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  • SPECIFIC KNOCKDOWN OF M-CALPAIN BLOCKS MYOGENESIS WITH cDNA DEDUCED FROM THE CORRESPONDING RNAi Reviewed

    Michiyo Honda, Teruhiko Toyo-oka, Takahide Tsuchiya, Nobuyuki Kanzawa

    JOURNAL OF PHYSIOLOGICAL SCIENCES   59   246 - 246   2009

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  • Specific knockdown of m-calpain blocks myogenesis with cDNA deduced from the corresponding RNAi.

    Honda M, Masui F, Kanzawa N, Tsuchiya T, Toyo-Oka T

    American journal of physiology   294 ( 4 )   957 - 965   2008.4

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  • Specific knockdown of m-calpain blocks myogenesis with cDNA deduced from the corresponding RNAi Reviewed

    Michiyo Honda, Fujiko Masui, Nobuyuki Kanzawa, Takahide Tsuchiya, Teruhiko Toyo-oka

    AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY   294 ( 4 )   C957 - C965   2008.4

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  • Comparative expression and tissue distribution analyses of astacin-like squid metalloprotease in squid and cuttlefish Reviewed

    Nobuyuki Kanzawa, Takuya Ogawa, Masanori Asakura, Keisuke Okiyama, Michiyo Honda, Takahide Tsuchiya

    ZOOLOGICAL SCIENCE   25 ( 1 )   14 - 21   2008.1

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  • Microenvironment of apatite-fiber scaffold affects cell proliferation and resulting cell differentiation Reviewed

    Michiyo Honda, Shigeki Izumi, Nobuyuki Kanzawa, Takahide Tsuchiya, Mamoru Aizawa

    BIOCERAMICS, VOL 20, PTS 1 AND 2   361-363   1075 - +   2008

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    Language：English   Publishing type：Research paper (international conference proceedings)  

    DOI： 10.4028/www.scientific.net/KEM.361-363.1075

    Web of Science

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  • Enhancement of the mechanical property of apatite-fiber scaffold using type I-collagen.

    S. Motojima, N. Igeta, Michiyo Honda, Nobuyuki Kanzawa, Mamoru Aizawa

    Key engineering materials   361-363   943 - 946   2007.10

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Trans Tech Pub  

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  • Osteogenic differentiation in a three-dimensional apatite-fiber scaffold.

    Honda M, Fujimi T.J, Kanzawa N, Izawa K, Tsuchiya T, Aizawa M

    Arch. BioCeram. Res.   6   171 - 174   2006

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    Language：English   Publishing type：Research paper (international conference proceedings)  

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  • Biochemical and molecular biological analysis of different responses to 2,3,7,8-tetrachlorodibenzo-p-dioxin in chick embryo heart and liver Reviewed

    N Kanzawa, M Kondo, T Okushima, M Yamaguchi, Y Temmei, M Honda, T Tsuchiya

    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS   427 ( 1 )   58 - 67   2004.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.abb.2004.04.021

    Web of Science

    PubMed

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MISC

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Research Projects

  • 血管新生を起点とする歯周組織再生技術の構築

    Grant number：21K12693  2021.4 - 2024.3

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    本田 みちよ

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    Grant amount：\4030000 （ Direct Cost: \3100000 、 Indirect Cost：\930000 ）

    生体機能を発現するための組織再生には，酸素や栄養素を供給できる「成熟した機能的な血管ネットワークの構築」と「生体内環境に類似し，細胞間相互作用を促進する三次元培養環境」を提供する必要がある。本研究では，血管新生を起点とした組織再生を実現するために，間葉系幹細胞MSC由来エクソソームに注目し，特に，MSCの持つ細胞特性を外部環境から制御し，優れた血管新生能を有する馴化培地やエクソソームを回収することを目指す。すなわち，MSCにより分泌される液性因子やエクソソームに注目して，効率的に血管新生を促進させる方法を模索する。今年度は，より多くの液性因子やエクソソームを回収するために，三次元培養が可能な材料（マイクロファイバーシート）上での細胞培養を実施した。三次元環境でのMSCの培養は細胞数の確保のみならず，細胞外マトリックス産生など，MSCが本来有する機能を高めることを可能とした。その一方で，多孔質の足場材料の構造は培養初期の細胞接着に大きく影響するため，基材の三次元構造の設計については再度検証する必要性があると考えられた。さらに，外部環境に加えて，MSCがより血管新生能を発現する物質を産生できるように，血管新生を促進させる遺伝子を恒常的に発現する細胞の作製についても取り組んでおり，遺伝子レベル，タンパク質レベルでの過剰発現を確認している。これらの条件で培養したMSCは，血管新生を向上させる機能を保持していると考えられ，組織再生に有用なアプローチとなると考えている。

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  • Construction of three-dimensional hard tissue with vascular network using a novel fusion process

    Grant number：17K01396  2017.4 - 2020.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Honda Michiyo

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    Grant amount：\4290000 （ Direct Cost: \3300000 、 Indirect Cost：\990000 ）

    The aim of this study is to construct bone-like tissue with three-dimensional structure by combination with biocompatible scaffold and periosteum-like sheet consisting of vascular endothelial cells and osteoblasts. The angiogenic proteins-loaded materials could enhance the cellular attachment through interaction with integrin and promote actin cytoskeletal reorganization. In the case of co-culture of osteoblasts and endothelial cells on a microfiber biomaterial, osteoblasts and endothelial cells could enhance and improve each other’s function such as osteoblastic differentiation and endothelial cell tube formation. These results were supported by the properties of the scaffold such as the fibrous and topological structures. This approach can provide the evidences for the effectiveness of the tissue-engineered bone with vascular networks by crosstalk between osteoblasts and endothelial cells and interaction between these cells and the scaffold.

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  • Regeneration of bone tissues in three-dimensional scaffold by controlling of vascularization

    Grant number：26750161  2014.4 - 2017.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (B)

    Honda Michiyo

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    Grant amount：\3640000 （ Direct Cost: \2800000 、 Indirect Cost：\840000 ）

    In the present study, we have fabricated three-dimensional apatite-fiber scaffold for regeneration of bone tissues. To possess the vascular network supplying cells with nutrients and oxygen, connective tissue growth factor (CTGF) was loaded on AFS. On the other hand, to create three-dimensional vascularized bone tissue, co-culture system (osteoblasts and endothelial cells) was employed. As a result, co-culture of osteoblasts and endothelial cells significantly increased alkaline phosphatase activity and expression of bone-related genes. Furthermore, abundant microcapillary-like structures were observed. Combination of these cell-based approaches and tissue engineering like three-dimensional scaffolds could provide a novel strategy for bone regeneration.

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  • Fabrication of artificial bone cements with anti-tumor effects and evaluation of their biological properties

    Grant number：22700500  2010 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (B)

    HONDA Michiyo

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    Grant amount：\3640000 （ Direct Cost: \2800000 、 Indirect Cost：\840000 ）

    In the present study, we have fabricated “chelate-setting apatite cements (IP6-HAp cements)” using HAp particles surface-modified with inositol hexaphosphate (IP6) and evaluated their anti-tumor effect. Osteosarcoma cultured on IP6-HAp cements (over 3000 ppm IP6) resulted in inhibition of cell growth, although normal cells grew well on those cements. These results led us to consider that locally high concentration of IP6 which was released from cement acts on the cells directly as anti-tumor agent and induces the apoptosis. Consequently, IP6-HAp cement might gain the anti-tumor effect and act as a carrier for local drug delivery system.

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