Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：食品化学新聞社  

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY  

DOI： 10.14814/phy2.13998

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Other Link： http://onlinelibrary.wiley.com/wol1/doi/10.14814/phy2.13998/fullpdf

Language：English   Publishing type：Research paper (scientific journal)  

High-intensity intermittent training (HIIT) is known to enhance mitochondrial biogenesis and energy metabolism in skeletal muscles. We aimed to elucidate the effects of HIIT on mitochondrial dynamics proteins, which is coupled with mitochondrial morphological adaptation. ICR mice (male, 8 weeks old) were divided into sedentary or HIIT groups (n=6 per group). The HIIT group underwent 10-12 sets of swimming for 20 s per set and inter-set intervals of 10 s, with a load of 10% body weight. Training sessions were conducted five times per week, and each training period lasted for 6 weeks. Gastrocnemius muscles were used for western blotting analysis. HIIT led to an increase in the expression of PGC-1αand OXPHOS proteins; however, Com- plex2 expression did not change. HIIT group showed higher ex- pressions of the mitochondrial fusion proteins Mfn2 and Opa1 and the mitochondrial fission protein Drp1 compared with that of the Sed group. The findings of this study indicate that HIIT enhances both mitochondrial biogenesis and mitochondrial dynamics-relat- ed proteins.

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Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：The Japanese Society of Physical Fitness and Sports Medicine  

<p>Eicosapentaenoic acid (EPA) is an omega-3 polyunsaturated fatty acid that is abundant in fish oil, and has anti-inflammatory or anti-obesity effects. However, the effects of EPA supplementation on mitochondrial content and dynamics (fusion and fission) in skeletal muscle has not been elucidated. We investigated the effects of EPA intake for 4 weeks on denervation-induced mitochondrial adaptation in mice skeletal muscle. ICR mice (male, 8 weeks old) were daily administrated olive oil (control) or EPA at a dose of 300 mg/kg body weight by gavage for 4 weeks. After 2 weeks of oil intake, mice underwent unilateral sciatic nerve transection surgery. The hindlimb without surgery served as the sham-operated control. Body and skeletal muscle weights did not differ between the control and the EPA groups. Peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α) and oxidative phosphorylation (OXPHOS) proteins were significantly decreased by denervation surgery. Denervation also induced the reduction of mitochondrial fusion molecules and the increase of mitochondrial fission molecules. Expression levels of PGC-1α and mitochondrial respiration protein MTCO1 were higher

DOI： 10.7600/jpfsm.7.261

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Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Japan Society of Physical Education, Health and Sport Sciences  

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Language：Japanese   Presentation type：Oral presentation (general)  

Venue：つくば市  

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Language：Japanese   Presentation type：Oral presentation (general)  

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Language：Japanese   Presentation type：Oral presentation (general)  

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Language：English   Presentation type：Oral presentation (general)  

Venue：USA San Diego  

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Language：Japanese   Presentation type：Oral presentation (general)  

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Award type：Honored in official journal of a scientific society, scientific journal 

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Award type：Award from Japanese society, conference, symposium, etc. 

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